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Determining light suppression of mitochondrial respiration for three temperate marine macrophytes using the Kok method

  • Lina M. Rasmusson ORCID logo EMAIL logo and Mats Björk
Published/Copyright: November 20, 2014

Abstract

To properly calculate the carbon budgets of coastal marine habitats, potential fluctuations in oxygen consumption due to mitochondrial respiration must be taken into account. As mitochondrial respiration is sometimes inhibited in light, we used the Kok method to estimate whether such suppression occurs in the seagrasses Zostera marina and Ruppia maritima as well as in the macroalga Ulva intestinalis. For Z. marina and U. intestinalis, the respiration rate was clearly downregulated in light, a finding that might be important when determining the impact of respiration on estimated net primary productivity in these marine habitats.


Corresponding author: Lina M. Rasmusson, Department of Ecology, Environment and Plant Sciences, Stockholm University, S-106 91 Stockholm, Sweden, e-mail: .

Acknowledgments

Funding by The Swedish Royal Academy of Sciences for the stay at SLC is gratefully acknowledged.

References

Amthor, J.S. 2000. The McCree-de Wit-Penning de Vries-Thornley respiration paradigms: 30 years later. Ann. Bot. 86: 1–20.10.1006/anbo.2000.1175Search in Google Scholar

Atkin, O.K., J.R. Evans, M.C. Ball, H. Lambers and L.P. Thijs. 2000. Leaf respiration of snow gum in the light and dark. Interactions between temperature and irradiance. Plant Physiol. 122: 915–923.10.1104/pp.122.3.915Search in Google Scholar

Barrón, C., C.M. Duarte, M. Frankignoulle and A. Vieira Borges. 2006. Organic carbon metabolism and carbonate dynamics in a Mediterranean seagrass (Posidonia oceanica) meadow. Estuaries Coasts 29: 417–425.10.1007/BF02784990Search in Google Scholar

Björk, M., F. Short, E. Mcleod and S. Beer. 2008. Managing seagrasses for resilience to climate change. IUCN, Gland, Switzerland. pp. 56.Search in Google Scholar

Brooks, A. and G.D. Farquhar. 1985. Effect of temperature on the CO2/O2 specificity of ribulose-1,5-biphosphate carboxylase/oxygenase and the rate of respiration in the light-estimates from gas-exchange measurements on spinach. Planta 165: 397–406.Search in Google Scholar

Bruhn, D., T.N. Mikkelsen, M. Herbst, W.L. Kutsch, M. Ball and K. Pilegaard. 2011. Estimating daytime ecosystem respiration from eddy-flux data. Biosystems 103: 309–313.10.1016/j.biosystems.2010.10.007Search in Google Scholar

Buapet, P., M. Gullström and M. Björk. 2013. Photosynthetic activity of seagrasses and macroalgae in temperate shallow waters can alter seawater pH and total inorganic carbon content at the scale of a coastal embayment. Mar. Freshw. Res. 64: 1040–1048.10.1071/MF12124Search in Google Scholar

Budde, J.A. and D.D. Randall. 1990. Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proc. Natl. Acad. Sci. USA 87: 673–676.10.1073/pnas.87.2.673Search in Google Scholar

Duarte, C.M., N. Marbà, E. Gacia, J.W. Fourqurean, J. Beggins, C. Barrón and E.T. Apostolaki. 2010. Seagrass community metabolism: assessing the carbon sink capacity of seagrass meadows. Global Biogeochem. Cycles 24, GB4032 24.Search in Google Scholar

Gemel, J. and D.D. Randall. 1992. Light regulation of leaf mitochondrial pyruvate dehydrogenase complex. Role of photorespiratory carbon metabolism. Plant Physiol. 100: 908–914.10.1104/pp.100.2.908Search in Google Scholar

Green, E.P. and F.T. Short. 2003. World atlas of seagrasses. University of California Press, Los Angeles. pp. 310.Search in Google Scholar

Hemminga, M.A. and C.M. Duarte. 2000. Seagrass ecology: an introduction. Cambridge University Press, Cambridge. pp. 298.Search in Google Scholar

Hurry, V., A.U. Igamberdiev, O. Keerberg, T. Pärnik, O.K. Atkin, J. Zaragoza-Castells and P. Gardeström. 2005. Respiration in photosynthetic cells: gas exchange components, interactions with photorespiration and the operation of mitochondria in the light. In: (H. Lambers and M. Ribas-Carbo, eds.) Plant respiration: from cell to ecosystem. Springer, Dordrecht. pp. 43–61.Search in Google Scholar

Igamberdiev, A.U. and P. Gardeström. 2003. Regulation of NAD- and NADP-dependent isocitrate dehydrogenases by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves. Biochim. Biophys. Acta 1606: 117–125.10.1016/S0005-2728(03)00106-3Search in Google Scholar

Kennedy, H. and M. Björk. 2009. Seagrass meadows. In: (D. d’A. Laffoley and G. Grimsditch, eds.) The management of natural coastal carbon sinks. IUCN, Gland. pp. 23–29.Search in Google Scholar

Kennedy, H., J. Beggins, C.M. Duarte, J.W. Fourqurean, M. Holmer, N. Marbà and J.J Middelburg. 2010. Seagrass sediments as a global carbon sink: isotopic constraints. Global Biogeochem. Cycles 24, GB4026.Search in Google Scholar

Kok, B. 1948. A critical consideration of the quantum yield of Chlorella photosynthesis. Enzymology 13: 1–56.Search in Google Scholar

Krömer, S. 1995. Respiration during photosynthesis. Ann. Rev. Plant Physiol. Plant Mol. Biol. 46: 45–70.10.1146/annurev.pp.46.060195.000401Search in Google Scholar

Laisk, A.K. 1977. Kinetics of photosynthesis and photorespiration in C3 plants. Nauka, Moscow (in Russian).Search in Google Scholar

Laisk, A.K. and F. Loreto. 1996. Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluorescence. Plant Physiol. 110: 903–912.10.1104/pp.110.3.903Search in Google Scholar PubMed PubMed Central

McCashin, B.G., E.A. Cossins and D.T. Canvin. 1988. Dark respiration during photosynthesis in wheat leaf slices. Plant Physiol. 87: 155–161.10.1104/pp.87.1.155Search in Google Scholar

Middelburg, J.J., C.M. Duarte and J.-P. Gattuso. 2005. Respiration in coastal benthic communities. In: (P.A. del Giorgio and P.J. le B Williams, eds.) Respiration in aquatic ecosystems. Oxford University Press, New York. pp. 206–224.Search in Google Scholar

Padmavathi, L. and A.S. Raghavendra. 2001. Importance of the cytochrome pathway of mitochondrial electron transport over the alternative pathway during the Kok effect in leaf discs of pea (Pisum sativum). Physiol. Plant 113: 430–434.10.1034/j.1399-3054.2001.1130318.xSearch in Google Scholar

Raghavendra, A.S., K. Padmasree and K. Saradadevi. 1994. Interdependence of photosynthesis and respiration in plant cells: interactions between chloroplasts and mitochondria. Plant Sci. 97: 1–14.10.1016/0168-9452(94)90101-5Search in Google Scholar

Ribas-Carbo, M., J. Flexas, S.A. Robinson and G.G.B. Tcherkez. 2010. Essay 11.9: in vivo measurement of plant respiration. Plant Physiol. Online: http://5e.plantphys.net/article.php?id=480.Search in Google Scholar

Ryan, M.G. 1991. Effects of climate change on respiration. Ecol. Appl. 1: 157–167.10.2307/1941808Search in Google Scholar PubMed

Shapiro, J.B., K.L. Griffin, J.D. Lewis and D.T. Tissue. 2004. Response of Xanthium strumarium leaf respiration in the light to elevated CO2 concentration, nitrogen availability and temperature. New Phytol. 162: 377–386.Search in Google Scholar

Sharp, R.E., M.A. Matthews and J.S. Boyer. 1984. Kok effect and the quantum yield of photosynthesis. Light partially inhibits dark respiration. Plant Physiol. 75: 95–101.10.1104/pp.75.1.95Search in Google Scholar PubMed PubMed Central

Tcherkez, G., G. Cornic, R. Bligny, E. Gout and J. Ghashghaie. 2005. In vivo respiratory metabolism of illuminated leaves. Plant Physiol. 138: 1596–1606.10.1104/pp.105.062141Search in Google Scholar PubMed PubMed Central

Tcherkez, G., R. Bligny, E. Gout, A. Mahé, M. Hodges and G. Cornic. 2008. Respiratory metabolism of illuminated leaves depends on CO2 and O2 conditions. Proc. Natl. Acad. Sci. USA 105: 797–802.10.1073/pnas.0708947105Search in Google Scholar PubMed PubMed Central

Tcherkez, G., E. Boex-Fontvieille, A. Mahé and M. Hodges. 2012a. Respiratory carbon fluxes in leaves. Curr. Opin. Plant Biol. 15: 308–314.10.1016/j.pbi.2011.12.003Search in Google Scholar PubMed

Tcherkez, G., A. Mahé, F. Guérard, E.R.A. Boex-Fontvieille, E. Gout, M. Lamothe, M.M. Barbour and R. Bligny. 2012b. Short-term effects of CO2 and O2 on citrate metabolism in illuminated leaves. Plant Cell Environ. 35: 2208–2220.10.1111/j.1365-3040.2012.02550.xSearch in Google Scholar PubMed

Tovar-Méndez, A., J.A. Miernyk and D.D. Randall. 2003. Regulation of pyruvate dehydrogenase complex activity in plant cells. Eur. J. Biochem. 270: 1043–1049.10.1046/j.1432-1033.2003.03469.xSearch in Google Scholar PubMed

Villar, R., A.A. Held and J. Merino. 1994. Comparison of methods to estimate dark respiration in the light in leaves of two woody species. Plant Physiol. 105: 167–172.10.1104/pp.105.1.167Search in Google Scholar PubMed PubMed Central

Yin, X., Z. Sun, P.C. Struik and J. Gu. 2011. Evaluating a new method to estimate the rate of leaf respiration in the light by analysis of combined gas exchange and chlorophyll fluorescence measurements. J. Exp. Bot. 62: 3489–3499.10.1093/jxb/err038Search in Google Scholar PubMed PubMed Central

Zou, D., K. Gao, J. Xia, X. Zhang and S. Liu. 2007. Responses of dark respiration in the light to desiccation and temperature in the intertidal macroalga, Ulva lactuca (Chlorophyta) during emersion. Phycologia 46: 363–370.Search in Google Scholar

Zou, D., K. Gao and J. Xia. 2011. Dark respiration in the light and in darkness of three marine macroalgal species grown under ambient and elevated CO2 concentrations. Acta Oceanol. Sin. 30: 1–7.10.1007/s13131-011-0096-5Search in Google Scholar

Received: 2014-7-25
Accepted: 2014-10-29
Published Online: 2014-11-20
Published in Print: 2014-12-1

©2014 by De Gruyter

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