Development and verification of a novel blood viscoelastic monitoring method based on reciprocating motion of magnetic bead

Xinyu Du; Fupan Chen; Lijin Gan; Yong Liu; Yu Zheng; Linghua Xing; Qi Zhou

doi:10.1515/bmt-2022-0225

Article

Development and verification of a novel blood viscoelastic monitoring method based on reciprocating motion of magnetic bead

Xinyu Du , Fupan Chen , Lijin Gan , Yong Liu , Yu Zheng , Linghua Xing and Qi Zhou

Published/Copyright: December 26, 2022

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From the journal Biomedical Engineering / Biomedizinische Technik Volume 68 Issue 2

Abstract

Blood coagulation function is an essential index in clinical examination, and it is of great significance to evaluate blood coagulation function comprehensively. Based on the blood viscoelasticity theory and hydrodynamics, we proposed a method to monitor the whole blood coagulation process based on the reciprocating motion of the magnetic bead (magnetic bead method for short). We have established a mathematical model between the moment acting on the magnetic bead and the viscoelasticity of blood in the process of blood coagulation. The change of blood viscoelasticity acks on the magnetic bead in the form of moment changes, which shows that the amplitude of the motion of the magnetic bead varies with the change of blood viscoelasticity. Designed and verified a blood coagulation monitoring device based on the reciprocating movement of the magnetic bead and discussed the device’s parameters through the orthogonal experiment. Lastly, the TEG5000 was used as the control group to test the thromboelasticity of four groups of thromboelastography quality control products in the same batch and 10 groups of human whole blood. It verified that our device has good repeatability, and has good consistency with TEG5000, it has particular application potential as a new blood coagulation monitoring method.

Keywords: angular displacement sensor; magnetic bead method; TEG5000; thromboelastography; whole blood coagulation

Corresponding author: Qi Zhou, Department of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China, E-mail: qizhou@cqut.edu.cn

Funding source: Graduate Innovation Foundation of Chongqing University of Technology

Award Identifier / Grant number: gzlcx20223348

Acknowledgments

We would like to express our gratitude to Chongqing South CNC equipment Co., Ltd. for providing reagents and TEG5000 equipment and helping us collect blood. We also thank the volunteers for their participation in this study.

Research funding: This work was funded by the Graduate Innovation Foundation of Chongqing University of Technology (No. gzlcx20223348). The funding organizations played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Author contributions: Xinyu Du: Formal analysis, Investigation, Methodology, Software, Writing – original draft, Writing – review & editing. Qi Zhou: Formal analysis, Methodology, Validation, Supervision. Fupan Chen: Formal analysis, Methodology, Software, Visualization. Lijin Gan: Methodology, Software, Writing – review & editing. Yong Liu: Software, Writing – review & editing. Yu Zheng: Software, Writing – review & editing. Linghua Xing: Software, Writing – review & editing.
Competing interests: Authors state no conflict of interest.
Informed consent: Before signing the experimental informed consent form, the subjects were informed of the content, nature, and purpose of the experiment, after which they all voluntarily signed the experimental informed consent form.
Ethical approval: The local Institutional Review Board deemed the study exempt from review. All the experiments were carried out in Chongqing South CNC equipment Co., Ltd.

References

1. Louka, M, Kaliviotis, E. Development of an optical method for the evaluation of whole blood coagulation. Biosensors 2021;11:113. https://doi.org/10.3390/bios11040113.Search in Google Scholar PubMed PubMed Central

2. Dahlbäck, B. Blood coagulation. Lancet 2000;355:1627–32. https://doi.org/10.1016/s0140-6736(00)02225-x.Search in Google Scholar

3. Ruggeri, ZM. The role of von Willebrand factor in thrombus formation. Thromb Res: Int J Vasc Obstruc Hemorr Hemost 2007;120:S5–9. https://doi.org/10.1016/j.thromres.2007.03.011.Search in Google Scholar PubMed PubMed Central

4. Collet, JP, Shuman, H, Ledger, RE, Lee, ST, Weisel, JW. The elasticity of an individual fibrin fiber in a clot. Proc Natl Acad Sci USA 2005;102:9133–7. https://doi.org/10.1073/pnas.0504120102.Search in Google Scholar PubMed PubMed Central

5. Thakur, M, Ahmed, AB, Technologies, A. A review of thromboelastography. Int J Ultrasound Appl Technol Perioperat Care 2012;1:25–9. https://doi.org/10.5005/jp-journals-10027-1006.Search in Google Scholar

6. Jennings, LK, Kotha, J. The utility of platelet and coagulation testing of antithrombotics: fusing science with patient care. Drug Dev Res 2013;74:587–93. https://doi.org/10.1002/ddr.21119.Search in Google Scholar PubMed PubMed Central

7. Herbstreit, F, Winter, EM, Peters, J, Hartmann, M. Monitoring of haemostasis in liver transplantation: comparison of laboratory based and point of care tests. Anaesthesia 2010;65:44–9. https://doi.org/10.1111/j.1365-2044.2009.06159.x.Search in Google Scholar PubMed

8. Bonnet, P, Devilliers, C, Saidi, K, Hausfater, P, Niclot, P, Ray, P, et al.. Impact de la seniorisation et du rappel des bonnes indications sur la prescription d’examens d’hémostase aux urgences pour adultes. Annales françaises de médecine d’urgence 2011;1:163–9. https://doi.org/10.1007/s13341-011-0045-4.Search in Google Scholar

9. Mulder, MB, Proctor, KG, Valle, EJ, Livingstone, AS, Nguyen, DM, Van Haren, RM. Hypercoagulability after resection of thoracic malignancy: a prospective evaluation. World J Surg 2019;43:3232–8. https://doi.org/10.1007/s00268-019-05123-7.Search in Google Scholar PubMed

10. Lippi, G, Favaloro, EJ, Franchini, M, Guidi, GC. Milestones and perspectives in coagulation and hemostasis. Semin Thromb Hemost 2009;35:9–22. https://doi.org/10.1055/s-0029-1214144.Search in Google Scholar PubMed

11. Barrowcliffe, TW, Raut, S, Sands, D, Hubbard, AR. Coagulation and chromogenic assays of factor VIII activity: general aspects, standardization, and recommendations. Semin Thromb Hemost 2002;28:247–56. https://doi.org/10.1055/s-2002-32658.Search in Google Scholar PubMed

12. Wersch, J. A chromogenic assay for coagulation factor VII: analytical performance characteristics and application in several diseases. Int J Clin Lab Res 1993;23:221. https://doi.org/10.1007/bf02592313.Search in Google Scholar PubMed

13. Lill, H, Spannagl, M, Trauner, A, Schramm, W, Schuller, D, Ofenloch-Haehnle, B, et al.. A new immunoassay for soluble fibrin enables a more sensitive detection of the activation state of blood coagulation in vivo. Blood Coagul Fibrinol: Int J Haemostasis Thromb 1993;4:97. https://doi.org/10.1097/00001721-199304010-00016.Search in Google Scholar

14. Bluth, MH, Kashuk, JL. Whole blood thromboelastometry: another Knight at the Roundtable? Critical Care (London, England) 2011;15:1021. https://doi.org/10.1186/cc10569.Search in Google Scholar PubMed PubMed Central

15. Lloyd-Donald, P, Churilov, L, Zia, F, Bellomo, R, Hart, G, McCall, P, et al.. Assessment of agreement and interchangeability between the TEG5000 and TEG6S thromboelastography haemostasis analysers: a prospective validation study. BMC Anesthesiol 2019;19:45. https://doi.org/10.1186/s12871-019-0717-7.Search in Google Scholar PubMed PubMed Central

16. Wikkelso, A, Wetterslev, J, Moller, AM, Afshari, A. Thromboelastography (TEG) or rotational thromboelastometry (ROTEM) to monitor haemostatic treatment in bleeding patients: a systematic review with meta-analysis and trial sequential analysis. Anaesthesia: J Assoc Anaesthet Great Britain and Ireland 2017;72:519–31. https://doi.org/10.1111/anae.13765.Search in Google Scholar PubMed

17. Tshikudi, DM, Tripathi, MM, Hajjarian, Z, Van Cott, EM, Nadkarni, SK. Optical sensing of anticoagulation status: towards point-of-care coagulation testing. PLoS One 2017;12:e0182491. https://doi.org/10.1371/journal.pone.0182491.Search in Google Scholar PubMed PubMed Central

18. Gosselin, RC, Estacio, EE, Song, JY, Dwyre, DM. Verifying the performance characteristics of the TEG5000 thromboelastogram in the clinical laboratory. Int J Lit Humanit 2016;38:183–92. https://doi.org/10.1111/ijlh.12464.Search in Google Scholar PubMed

19. Evans, PA, Hawkins, KM, Lawrence, MJ, Williams, PR, Williams, RL, Co, A, et al.. Rheometrical studies of blood clot formation by oscillatory shear, thromboelastography, sonoclot analysis and free oscillation rheometry. In: AIP conference proceedings 2008:597–9 pp.10.1063/1.2964777Search in Google Scholar

20. Ganter, MT, Hofer, CK. Coagulation monitoring: current techniques and clinical use of viscoelastic point-of-care coagulation devices. Anesth Analg: J Int Anesth Res Soc 2008;106:1366–75. https://doi.org/10.1213/ane.0b013e318168b367.Search in Google Scholar PubMed

21. Solomon, C, Schöchl, H, Ranucci, M, Schött, U, Schlimp, CJ. Comparison of fibrin-based clot elasticity parameters measured by free oscillation rheometry (ReoRox®) versus thromboelastometry (ROTEM®). Scand J Clin Lab Investig 2015;75:239–46. https://doi.org/10.3109/00365513.2014.993698.Search in Google Scholar PubMed PubMed Central

22. Solbeck, S, Windelov, NA, Baek, NH, Nielsen, JD, Ostrowski, SR, Johansson, PI. In-vitro comparison of free oscillation rheometry (ReoRox) and rotational thromboelastometry (ROTEM) in trauma patients upon hospital admission. Blood Coagul Fibrinolysis 2012;23:688–92. https://doi.org/10.1097/mbc.0b013e328351ebd6.Search in Google Scholar PubMed

23. Winstedt, D, Tynngård, N, Olanders, K, Schött, U. Free oscillation rheometry monitoring of haemodilution and hypothermia and correction with fibrinogen and factor XIII concentrates. Scand J Trauma Resuscitation Emerg Med 2013;21:20. https://doi.org/10.1186/1757-7241-21-20.Search in Google Scholar PubMed PubMed Central

24. Tynngard, N, Berlin, G, Samuelsson, A, Berg, S. Low dose of hydroxyethyl starch impairs clot formation as assessed by viscoelastic devices. Scand J Clin Lab Invest 2014;74:344–50. https://doi.org/10.3109/00365513.2014.891259.Search in Google Scholar PubMed

25. Gurbel, PA, Bliden, KP, Tantry, US, Monroe, AL, Muresan, AA, Brunner, NE, et al.. First report of the point-of-care TEG: a technical validation study of the TEG-6S system. Platelets 2016;27:642–9. https://doi.org/10.3109/09537104.2016.1153617.Search in Google Scholar PubMed

26. Kang, YJ. Blood viscoelasticity measurement using interface variations in coflowing streams under pulsatile blood flows. Micromachines 2020;11:245. https://doi.org/10.3390/mi11030245.Search in Google Scholar PubMed PubMed Central

27. Whiting, D, Dinardo, JA. TEG and ROTEM: technology and clinical applications. Am J Hematol 2014;89:228–32. https://doi.org/10.1002/ajh.23599.Search in Google Scholar PubMed

28. Neal, MD, Moore, EE, Walsh, M, Thomas, S, Callcut, RA, Kornblith, LZ, et al.. A comparison between the TEG 6s and TEG 5000 analyzers to assess coagulation in trauma patients. J Trauma Acute Care Surg 2020;88:279–85. https://doi.org/10.1097/ta.0000000000002545.Search in Google Scholar PubMed PubMed Central

29. Hoffman, M, Monroe, DM3rd. A cell-based model of hemostasis. Thromb Haemostasis: J Int Soc Thromb Haemostasis 2001;85:958–65. https://doi.org/10.1055/s-0037-1615947.Search in Google Scholar

30. Romney, G, Glick, M. An updated concept of coagulation with clinical implications. J Am Dent Assoc 2009;140:567–74. https://doi.org/10.14219/jada.archive.2009.0227.Search in Google Scholar PubMed

31. Negrier, C, Shima, M, Hoffman, M. The central role of thrombin in bleeding disorders. Blood Rev 2019;38:100582. https://doi.org/10.1016/j.blre.2019.05.006.Search in Google Scholar PubMed

32. Bjarne, Ø, Elvin, H, Samuel, IR, Kathrine, KL. Evidence against collagen activation of platelet associated factor XI as a mechanism for initiating intrinsic clotting. Scand J Haematol 1979;22:205–13.10.1111/j.1600-0609.1979.tb02798.xSearch in Google Scholar PubMed

33. Conway, EM. Thrombin: coagulation’s master regulator of innate immunity. J Thromb Haemostasis 2019;17:1785–9. https://doi.org/10.1111/jth.14586.Search in Google Scholar PubMed

34. Cui, J, Miao, XJ. The relation of shear rate and velocity gradient. J Inner Mongolia Univ Nationalities (Nat Sci) 2012;27:9–10. https://doi.org/10.14045/j.cnki.15-1220.2012.01.002.Search in Google Scholar

35. Thurston, GB. Frequency and shear rate dependence of viscoelasticity of human blood. Biorheology 1973;10:375–81. https://doi.org/10.3233/bir-1973-10311.Search in Google Scholar PubMed

36. Affeld, K, Goubergrits, L, Watanabe, N, Kertzscher, U. Numerical and experimental evaluation of platelet deposition to collagen coated surface at low shear rates. J Biomech 2013;46:430–6. https://doi.org/10.1016/j.jbiomech.2012.10.030.Search in Google Scholar PubMed

37. Grima, A, Di Castro, M, Masi, A, Sammut, N. Design enhancements of an ironless inductive position sensor. IEEE Trans Instrum Meas 2020;69:1362–9. https://doi.org/10.1109/tim.2019.2911759.Search in Google Scholar

38. Guo, YX, Lai, C, Shao, ZB, Xu, KL, Li, T. Differential structure of inductive proximity sensor. Sensors 2019;19:2210. https://doi.org/10.3390/s19092210.Search in Google Scholar PubMed PubMed Central

39. Giavarina, D. Understanding Bland Altman analysis. Biochem Med 2015;25:141–51. https://doi.org/10.11613/bm.2015.015.Search in Google Scholar

Received: 2022-06-12

Accepted: 2022-12-12

Published Online: 2022-12-26

Published in Print: 2023-04-25

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Articles in the same Issue

https://doi.org/10.1515/bmt-2022-0225

Keywords for this article

angular displacement sensor; magnetic bead method; TEG5000; thromboelastography; whole blood coagulation