Modulation of neo-endothelialization of vascular graft materials by silk fibroin
-
Congcong Zhan
Abstract
Controlled neo-endothelialization is critical to the patency of vascular grafts. Expanded polyethylene terephthalate (PET) vascular grafts were grafted with polyethylene glycol (PEG), irradiated with ultraviolet light, and subsequently coated with silk fibroin (SF) and EDC in a dip-coating process. Endothelial cells were cultivated on the coated samples for 1, 3, 5, and 7 days, and characterized by fluorescence microscopy and scanning electron microscopy (SEM). The quantitative analyse of CCK-8 method was used to assess ECs proliferation. The results reveal the correlation between grafting components and cell adhesion. We demonstrated that PET with SF grafting facilitated cell adhesion and spreading. Following 7 days of cell culture in vitro, PET-PEG6000-SF (PEG molecular weight 6,000) displayed spreading of cells over a significantly larger area. Rapid endothelialization on a modified PET surface resulted in large tissue pack that can be observed by SEM.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: No. 51202221
Acknowledgments
The authors would like to thank the authors of all the published research that contributed to the data used in this study.
-
Research funding: This research was supported by National Natural Science Foundation of China (No. 51202221).
-
Author contributions: X.-C.H. Z.- C.C and H.X. designed the experiment, analyzed the data, and wrote the paper. W.-P.F., X.-C.H. and M.-P.D. performed the experiments. Z.-S.F. and C.-J.Y. commented the manuscript. All authors have read and approved the manuscript.
-
Competing interests: The authors declare that they have no competing interests.
-
Informed consent: Informed consent was obtained from all individuals included in this study.
-
Ethical approval: The local Institutional Review Board deemed the study exempt from review.
References
1. Lovett, M, Cannizzaro, C, Daheron, L, Messmer, B, Vunjak-Novakovic, G, Kaplan, DL. Silk fibroin microtubes for blood vessel engineering. Biomaterials 2007;28:5271–9. https://doi.org/10.1016/j.biomaterials.2007.08.008.Search in Google Scholar PubMed PubMed Central
2. Bourke, H, Gordon, D, Salmon, L, Waller, A, Linklater, J, Pinczewski, L. The outcome at 15 years of endoscopic anterior cruciate ligament reconstruction using hamstring tendon autograft for ‘isolated’anterior cruciate ligament rupture. J Bone Joint Surg Br 2012;94:630–7. https://doi.org/10.1302/0301-620x.94b5.28675.Search in Google Scholar
3. Claes, S, Verdonk, P, Forsyth, R, Bellemans, J. The “ligamentization” process in anterior cruciate ligament reconstruction: what happens to the human graft? A systematic review of the literature. Am J Sports Med 2011;39:2476–83. https://doi.org/10.1177/0363546511402662.Search in Google Scholar PubMed
4. Yamamoto, K, Tomita, N, Fukuda, Y, Suzuki, S, Igarashi, N, Suguro, T, et al.. Time-dependent changes in adhesive force between chondrocytes and silk fibroin substrate. Biomaterials 2007;28:1838–46. https://doi.org/10.1016/j.biomaterials.2006.11.045.Search in Google Scholar PubMed
5. Furuzono, T, Masuda, M, Okada, M, Yasuda, S, Kadono, H, Tanaka, R, et al.. Increase in cell adhesiveness on a poly (ethylene terephthalate) fabric by sintered hydroxyapatite nanocrystal coating in the development of an artificial blood vessel. ASAIO J 2006;52:315–20. https://doi.org/10.1097/01.mat.0000214860.08820.f9.Search in Google Scholar PubMed
6. Wang, F, Tong, Z. Solely biomass‐derived polyethylene terephthalate (PET): conversion of bio‐based isoprene and acrolein to p‐xylene and terephthalic acid. Chemistryselect 2016;1:5538–41. https://doi.org/10.1002/slct.201600055.Search in Google Scholar
7. Rockwood, DN, Preda, RC, Yücel, T, Wang, XQ, Lovett, ML, Kaplan, DL. Materials fabrication from Bombyx mori silk fibroin. Nat Protoc 2011;6:1612–31. https://doi.org/10.1038/nprot.2011.379.Search in Google Scholar PubMed PubMed Central
8. Li, H, Ge, Y, Wu, Y, Jiang, J, Gao, K, Zhang, P, et al.. Hydroxyapatite coating enhances polyethylene terephthalate artificial ligament graft osseointegration in the bone tunnel. Int Orthop 2011;35:1561–7. https://doi.org/10.1007/s00264-010-1158-6.Search in Google Scholar PubMed PubMed Central
9. Chen, K, Shi, P, Teh, TKH, Toh, SL, Goh, JC. In vitro generation of a multilayered osteochondral construct with an osteochondral interface using rabbit bone marrow stromal cells and a silk peptide-based scaffold. J Tissue Eng Regen Med 2016;10:284–93. https://doi.org/10.1002/term.1708.Search in Google Scholar PubMed
10. Farè, S, Torricelli, P, Giavaresi, G, Bertoldi, S, Alessandrino, A, Villa, T, et al.. In vitro study on silk fibroin textile structure for anterior cruciate ligament regeneration. Mater Sci Eng C 2013;33:3601–8. https://doi.org/10.1016/j.msec.2013.04.027.Search in Google Scholar PubMed
11. Fan, H, Liu, H, Toh, SL, Goh, JC. Anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold in large animal model. Biomaterials 2009;30:4967–77. https://doi.org/10.1016/j.biomaterials.2009.05.048.Search in Google Scholar PubMed
12. Wang, X, Kim, HJ, Peng, X, Matsumoto, A, Kaplan, DL. Biomaterial coatings by stepwise deposition of silk fibroin. Langmuir 2005;21:11335–41. https://doi.org/10.1021/la051862m.Search in Google Scholar PubMed
13. Karageorgiou, V, Meinel, L, Hofmann, S, Malhotra, A, Volloch, V, Kaplan, D. Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells. J Biomed Mater Res 2004;71:528–37. https://doi.org/10.1002/jbm.a.30186.Search in Google Scholar PubMed
14. Aytemiz, D, Asakura, T. Biologically-inspired systems. In: Application of Bombyx mori silk fibroin as a biomaterial for vascular grafts. Berlin: Springer; 2014, vol 5.10.1007/978-94-007-7119-2_4Search in Google Scholar
15. Li, Y, Huang, G, Zhang, X, Wang, L, Du, Y, Lu, TJ, et al.. Engineering cell alignment in vitro. Biotechnol Adv 2014;32:347–65. https://doi.org/10.1016/j.biotechadv.2013.11.007.Search in Google Scholar PubMed
16. Zheng, JJ, Yang, D, Qin, X, Shi, GJ, Ji, LJ. Synthesis of an organic-inorganic hybrid with short organic molecular chains by sol-gel chemistry. J Mech Behav Biomed Mater 2021;120:104567. https://doi.org/10.1016/j.jmbbm.2021.104567.Search in Google Scholar PubMed
17. Li, F, Wang, J, Sun, H, Huang, N. Surface characterization and in vitro blood compatibility of poly(ethylene terephthalate) immobilized with hirudin. Key Eng Mater 2005;288/289:421–24. https://doi.org/10.1021/bm9006503.Search in Google Scholar PubMed
18. Zhang, H, Li, W, Huang, R, Wang, JP. Effects of polyvinyl alcohol modification on microstructure, thermal properties and impermeability of microencapsulated n-dodecanol as phase change material. Chemistryselect 2017;2:9369–76. https://doi.org/10.1002/slct.201701529.Search in Google Scholar
19. McBeath, R, Pirone, DM, Nelson, CM, Bhadriraju, K, Chen, CS. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 2004;6:483–95. https://doi.org/10.1016/s1534-5807(04)00075-9.Search in Google Scholar PubMed
20. Tay, CY, Pal, M, Yu, H, Leong, WS, Tan, NS, Ng, KW, et al.. Bio-inspired micropatterned platform to steer stem cell differentiation. Small 2011;7:1416–21. https://doi.org/10.1002/smll.201002298.Search in Google Scholar PubMed
21. Bodenberger, N, Paul, P, Kubiczek, D, Walther, P, Gottschalk, K, Rosenau, F. A novel cheap and easy to handle protein hydrogel for 3D cell culture applications: a high stability matrix with tunable elasticity and cell adhesion properties. Chemistryselect 2016;1:1353–60. https://doi.org/10.1002/slct.201600206.Search in Google Scholar
22. Bobrowska, DM, Czyrko, J, Eljaszewicz, A, Grubczak, K, Wlodarczyk, AJ, Moniuszko, M, et al.. 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose: its anti-inflammatory and antibacterial properties. Chemistryselect 2018;3:2498–501. https://doi.org/10.1002/slct.201703001.Search in Google Scholar
23. Silva, R, Cavicchioli, M, Lima, LR, Otoni, CG, Barud, HS, Santagneli, SH, et al.. Fabrication of biocompatible, functional, and transparent hybrid films based on silk fibroin and epoxy silane for biophotonics. ACS Appl Mater Interfaces 2017;9:27905–17. https://doi.org/10.1021/acsami.7b06061.Search in Google Scholar PubMed
24. Sylwia Grabska-Zielińska, S, Sionkowska, A, Reczyńska, K, Pamuła, E. Physico-chemical characterization and biological tests of collagen/silk fibroin/chitosan scaffolds cross-linked by dialdehyde starch. Polymers 2020;12:1–14.10.3390/polym12020372Search in Google Scholar PubMed PubMed Central
25. Sionkowska, A, Grabska, S, Lewandowska, K, Andrzejczyk, A. Polymer films based on silk fibroin and collagen- the physico-chemical properties. Mol Cryst Liq Cryst 2016;640:13–20. https://doi.org/10.1080/15421406.2016.1255084.Search in Google Scholar
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Reliability and validity varies among smartphone apps for range of motion measurements of the lower extremity: a systematic review
- Research Articles
- Extended measuring depth dual-wavelength Fourier domain optical coherence tomography
- Linear and non-linear feature extraction from rat electrocorticograms for seizure detection by support vector machine
- Modulation of neo-endothelialization of vascular graft materials by silk fibroin
- Hippocampus segmentation and classification for dementia analysis using pre-trained neural network models
- Modular 3D printable orthodontic measuring apparatus for force and torque measurements of thermoplastic/removable appliances
- Therapeutic maps for a sensor-based evaluation of deep brain stimulation programming
- Voice pathology detection and classification from speech signals and EGG signals based on a multimodal fusion method
Articles in the same Issue
- Frontmatter
- Review
- Reliability and validity varies among smartphone apps for range of motion measurements of the lower extremity: a systematic review
- Research Articles
- Extended measuring depth dual-wavelength Fourier domain optical coherence tomography
- Linear and non-linear feature extraction from rat electrocorticograms for seizure detection by support vector machine
- Modulation of neo-endothelialization of vascular graft materials by silk fibroin
- Hippocampus segmentation and classification for dementia analysis using pre-trained neural network models
- Modular 3D printable orthodontic measuring apparatus for force and torque measurements of thermoplastic/removable appliances
- Therapeutic maps for a sensor-based evaluation of deep brain stimulation programming
- Voice pathology detection and classification from speech signals and EGG signals based on a multimodal fusion method
