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Dynamic cerebral perfusion parameters and magnetic nanoparticle accumulation assessed by AC biosusceptometry

  • André Gonçalves Próspero ORCID logo EMAIL logo , Guilherme Augusto Soares , Gustavo Morlin Moretto , Caio C. Quini , Andris Figueiroa Bakuzis and José Ricardo de Arruda Miranda
Published/Copyright: November 12, 2019

Abstract

Cerebral blood flow (CBF) assessment is mainly performed by scintigraphy, computed tomography (CT) and magnetic resonance imaging (MRI). New approaches to assess the CBF through the passage of magnetic nanoparticles (MNPs) to blood-brain barrier (BBB) are convenient to help decrease the use of ionizing radiation and unleash the required MRI schedule in clinics. The development of nanomedicine and new biomedical devices, such as the magnetic particle imaging (MPI), enabled new approaches to study dynamic brain blood flow. In this paper, we employed MNPs and the alternating current biosusceptometry (ACB) to study the brain perfusion. We utilized the mannitol, before the MNPs, injection to modulate the BBB permeability and study its effects on the circulation time of the MNPs in the brain of rats. Also, we characterized a new ACB sensor to increase the systems’ applicability to study the MNPs’ accumulation, especially in the animals’ brain. Our data showed that the injection of mannitol increased the circulation time of MNPs in the brain. Also, the mannitol increased the accumulation of MNPs in the brain. This paper suggests the use of the ACB as a tool to study brain perfusion and accumulation of MNPs in studies of new nano agents focused on the brain diagnostics and treatment.

Funding source: FAPESP

Award Identifier / Grant number: 2015/149239

Funding statement: Coordination for the Improvement of Higher Education Personnel (CAPES), São Paulo Research Foundation (FAPESP – project number 2015/149239, funder Id: http://dx.doi.org/10.13039/501100001807) and National Council for Scientific and Technological Development (CNPq) are acknowledged for the financial support.

  1. Author statement

  2. Conflict of interest: Authors state no conflict of interest.

  3. Informed consent: Informed consent is not applicable.

  4. Ethical approval: The research related to animals’ use complied with the National Council for the Control of Animal Experimentation (CONCEA – Brazil) and ethical principles in animal research formulated by the Brazilian Society of Science in Laboratory Animals, and was approved by the Biosciences Institute/UNESP Ethics Committee on Use of Animals (CEUA) under the local protocol number 802.

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Received: 2019-04-16
Accepted: 2019-08-30
Published Online: 2019-11-12
Published in Print: 2020-05-26

©2019 Walter de Gruyter GmbH, Berlin/Boston

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