Startseite Lebenswissenschaften Distinct expression patterns of the GDP dissociation inhibitor protein gene (OsRhoGDI2) from Oryza sativa during development and abiotic stresses
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Distinct expression patterns of the GDP dissociation inhibitor protein gene (OsRhoGDI2) from Oryza sativa during development and abiotic stresses

  • Jun-jun Huang , Jing Zhang , Yu-fan Hao , Xin-tian Yan , Jia Shi , Gao-hua Wang , Jing-yao Du , Hui-wen Ge , Hua-hua Wang und Wei-hong Liang EMAIL logo
Veröffentlicht/Copyright: 23. Dezember 2016
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Biologia
Aus der Zeitschrift Biologia Band 71 Heft 11

Abstract

Guanine nucleotide dissociation inhibitor (GDI) plays an essential role in regulating the forms of Rac/Rop between GDP-bound inactivity and GTP-bound activity in plants. In this paper, we reported a stress-responsive GDI gene (OsRhoGDI2) from rice (Oryza sativa L.). We analyzed the transcript levels of OsRhoGDI2 gene in various tissues, organs, and developmental stages to obtain information about its function. We further researched the expression patterns of OsRhoGDI2 gene in response to abiotic stress signals. qRT-PCR demonstrated that OsRhoGDI2 was distinctly expressed in various plant tissues and organs at different levels. The expression of OsRhoGDI2 was also highly salty and drought inducible, it also moderately responded to Methyl Jasmonate (MeJA), abscisic acid (ABA), and Indole-3-acetic Acid (IAA), treatment but was only slightly affected by 6-Benzylaminopurine (6-BA) and salicylic acid (SA) treatments. Nevertheless, reduced expression conferred hypersensitivity to gibberellin (GA) stress in rice. The promoter of OsRhoGDI2 gene was used to drive β-glucuronidase (GUS) gene expression. Results of GUS histochemical staining showed the tissue-specific expression patterns of OsRhoGDI2, and GUS gene expression in two-week-old transgenic rice seedling exhibited relatively similar patterns under different stresses of the transgenic rice lines. These results provided insights into the characteristics and roles of the OsRhoGDI2 gene during development and strongly suggested that OsRhoGDI2 may play direct or indirect roles in the tolerance to different stresses in rice and may serve as a basis for further functional studies.

Keywords: Oryza sativa; GUS; OsRhoGDI2; stress response; transgenic rice

Acknowledgements

This work was supported by the research grants from the National Science Foundation of China (31171182; U1204305; 31301252), Program for Innovative Research Team in Science and Technology in University of Henan Province (13IRTSTHN009; 15IRTSTHN020) and the Doctor Initiative Foundation of Henan Normal University (Nos 11126, 11129).

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Received: 2016-6-23
Accepted: 2016-9-19
Published Online: 2016-12-23
Published in Print: 2016-11-1

© 2016 Institute of Botany, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0146
Schlagwörter für diesen Artikel
Oryza sativa; GUS; OsRhoGDI2; stress response; transgenic rice

Artikel in diesem Heft

  1. Cellular and Molecular Biology
  2. The evolutionary pathway of the staphylococcal cassette chromosome element
  3. Cellular and Molecular Biology
  4. Detection of the antibacterial effect of Chaetomium cochliodes Palliser CCM F-232 based on agar plugs and unprocessed fungal substances from cultivation media
  5. Botany
  6. Identification and molecular characterization of one novel 1Sl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)
  7. Botany
  8. Bioinformatic analysis of Arabidopsis reverse transcriptases with a zinc-finger domain
  9. Botany
  10. Distinct expression patterns of the GDP dissociation inhibitor protein gene (OsRhoGDI2) from Oryza sativa during development and abiotic stresses
  11. Botany
  12. An application of genetics-chemicals constituents to the relatedness of three Euphorbia species
  13. Zoology
  14. Centipede (Chilopoda) richness, diversity and community structure in the forest-steppe nature reserve “Bielinek” on the Odra River (NW Poland, Central Europe)
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  16. Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI
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  18. A faunistic study on Carabidae and Scarabaeidae in alfalfa fields from Central Greece
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  24. Temperature and precipitation effects on breeding productivity of some passerines – a multivariate analysis of constant effort mist-netting data
  25. Cellular and Molecular Biology
  26. The direct action of hyaluronic acid on human U-937 and HL-60 cells – modification of native and model membranes
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