Home Life Sciences ASL16 gene, a member of AS2/LOB family, is essential for lateral root formation in Arabidopsis
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ASL16 gene, a member of AS2/LOB family, is essential for lateral root formation in Arabidopsis

  • Lai-Sheng Meng EMAIL logo , He Bizhu , Xiao-Ying Cao , Xu-Qin Yang , Jiang-Ping Song , Yi-Bo Wang and Ji-Hong Jiang
Published/Copyright: September 17, 2016
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Abstract

ASYMMETRIC LEAVES2-LIKE16 gene of Arabidopsis is a member of the ASYMMETRIC LEAVES2 (AS2)/LATERAL ORGAN BOUNDARIES (LOB) domain gene family. Current reports have indicated that overexpression of ASL16 induces lateral root formation in the absence of ARF7 and ARF19; whereas lateral root formation is severely reduced in the arf 7 and arf 19 double knockout mutants. Antisense mRNA has testified to be a useful tool to inhibit the expression of specific genes in plants; more significantly, it is specific in the sense that only the mRNA level of the desired protein being changed, while other members of the gene family remain unaffected. To further explore ASL16 function in lateral root growth and development, we introduced an antisense-expression construct of the ASL16 gene to Arabidopsis (Col-0); and acquired some 35S:ASL16 Arabidopsis plants. With 11 day after germination (DAG), many lateral roots in wild-type seedlings were well developed, whereas a few lateral roots in these antisense 35S:ASL16 seedlings were produced. However, with 5, 8 and 16 DAG, the lateral roots in these transgenic seedlings were not distinct with those of wild-type plants. A series of data indicate that primary root formation of these antisense 35S:ASL16 plants was not affected; whereas their lateral root formation was severely inhibited.


§ contributed equally to this work


Acknowledgements

This research was supported by the Youth Fund of the Natural Science Foundation of Jiangsu Province of China (BK20150232), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (15KJD180004), Grants from Natural Science Foundation by Jiangsu Normal University (15XLR025). This study was also supported by grants from the National Science Foundation of China (31401443, 31370646; 31560164).

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Abbreviations
RT-PCR

reverse transcription PCR

WT

wild type

M

marker

DAG

day after germination

Received: 2016-6-5
Accepted: 2016-8-2
Published Online: 2016-9-17
Published in Print: 2016-9-1

©2016 Institute of Botany, Slovak Academy of Sciences

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