Non-genomic action of TCDD to induce inflammatory responses in HepG2 human hepatoma cells and in liver of C57BL/6J mice
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Wen Li
Abstract
To assess the significance of the non-genomic signaling of TCDD (=dioxin) on liver of C57BL/6 mice and HepG2 human hepatoma cells, we first determined the group of markers that are susceptible to inhibition by parthenolide, a compound known to specifically suppress NF-κB-mediated inflammation. Of those, the most consistent marker turned out to be SOCS3 (a suppressor of cytokine signaling) known to respond to inflammation. An early diagnostic test on the action of TCDD on HepG2 cells in vitro within 3–6 h indicated that Cox-2 and SOCS3 are mainly induced via a non-genomic route, whereas PAI-2 appears to be induced through the classical action route. More detailed diagnostic tests at later stages of action of TCDD in HepG2 cells revealed that induction of IL-1β, BAFF, and iNOS are largely mediated by the protein kinase-dependent non-genomic route. An in vivo study on the 7 day action of TCDD on liver of AhRNLS mice showed that several early markers (e.g., Cox-2, MCP-1 and SOCS3) are induced, but not late markers such as IL-1β. Together, these results show that the non-genomic pathway contributes significantly to the early stress response reactions to TCDD that includes inflammation in hepatoma cells as well as in the liver.
©2010 by Walter de Gruyter Berlin New York
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- Guest Editorial
- Highlight: The Biology of Aging: Mechanisms and Intervention
- Highlight: 61th Mosbach Colloquium of the GBM ‘The Biology of Aging: Mechanisms and Intervention’
- Multiplex analysis of mitochondrial DNA pathogenic and polymorphic sequence variants
- The hypoxia-inducible factor HIF-1 functions as both a positive and negative modulator of aging
- E. coli hypoxia-inducible factor ArcA mediates lifespan extension in a lipoic acid synthase mutant by suppressing acetyl-CoA synthetase
- Glucose homeostasis and insulin sensitivity in growth hormone-transgenic mice: a cross-sectional analysis
- PROTEIN STRUCTURE AND FUNCTION
- New structural aspects of FKBP38 activation
- The neuronal proteins CIPP, Cypin and IRSp53 form a tripartite complex mediated by PDZ and SH3 domains
- CELL BIOLOGY AND SIGNALING
- Inhibition of interferon-α-induced signaling by hyperosmolarity and hydrophobic bile acids
- Role of the second disulfide bridge (Cys18-Cys274) in stabilizing the inactive AT1 receptor
- Demonstration of protein absorption in the intestinal epithelium of fish and mice by laser scanning confocal microscopy
- Non-genomic action of TCDD to induce inflammatory responses in HepG2 human hepatoma cells and in liver of C57BL/6J mice
- PROTEOLYSIS
- Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases
