Effects of magnesium ions on recombinant human furin: selective activation of hydrolytic activity upon substrates derived from virus envelope glycoprotein
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Mario A. Izidoro
Abstract
Here we report a detailed analysis of magnesium (Mg2+) ion effects on furin hydrolysis of fluorescent resonance energy transfer decapeptide substrates derived from canonical R-X-K/R-R furin cleavage motifs within certain viral envelope glycoproteins and eukaryotic proproteins. Using virus-derived sequences a selective activation of furin by Mg2+ ions was observed as a result of cooperativity between furin subsites. Furin hydrolysis of the peptides Abz-SRRHKR↓FAGV-Q-EDDnp (from measles virus fusion protein Fo) and Abz-RERRRKKR↓GLFG-Q-EDDnp (from Asian avian influenza A, H5N1) was activated between 60- and 80-fold by MgCl2. It appears that virus envelope glycoprotein mutations have been selected to increase their susceptibility to furin within cells, a location where Mg2+ is present in adequate concentrations for activation. Both the pH profile of furin and its intrinsic fluorescence were modified by Mg2+ ions, which bind to furin with a Kd value of 1.1 mm.
©2010 by Walter de Gruyter Berlin New York
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- Molecular mechanisms of signal transduction via adiponectin and adiponectin receptors
- Genes and Nucleic Acids
- Isolation of genes involved in pancreas regeneration by subtractive hybridization
- Wild type p53-dependent transcriptional upregulation of cathepsin L expression is mediated by C/EBPα in human glioblastoma cells
- Protein Structure and Function
- Epstein-Barr virus lytic cycle activation alters proteasome subunit expression in Burkitt's lymphoma cells
- Dentipain, a Streptococcus pyogenes IdeS protease homolog, is a novel virulence factor of Treponema denticola
- Membranes, Lipids, Glycobiology
- Resveratrol treatment induces redox stress in red blood cells: a possible role of caspase 3 in metabolism and anion transport
- Cell Biology and Signaling
- Activation of the plasma kallikrein-kinin system on human lung epithelial cells
- Antiproliferative effects induced by guanine-based purines require hypoxanthine-guanine phosphoribosyltransferase activity
- Phosphorylation of caldesmon by myosin light chain kinase increases its binding affinity for phosphorylated myosin filaments
- Proteolysis
- Effects of magnesium ions on recombinant human furin: selective activation of hydrolytic activity upon substrates derived from virus envelope glycoprotein
