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Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study

  • Martin Kruså , Gunnar Henriksson , Gunnar Johansson , Torbjörn Reitberger and Helena Lennholm
Published/Copyright: July 5, 2005
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Holzforschung
From the journal Volume 59 Issue 3

Abstract

Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe3+ to Fe2+. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl β-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.

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Corresponding author. Department of Fiber and Polymer Technology, Royal Institute of Technology, 53, 100 44 Stockholm, KTH, Drottning Kristinas väg, Sweden Fax: +46-8-790-6166 E-mail:

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Published Online: 2005-07-05
Published in Print: 2005-05-01

© by Walter de Gruyter Berlin New York

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  2. Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study
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