Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study
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Martin Kruså
, Gunnar Henriksson , Gunnar Johansson , Torbjörn Reitberger and Helena Lennholm
Abstract
Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidizes cellobiose to cellobionolactone under reduction of molecular oxygen to hydrogen peroxide, and Fe3+ to Fe2+. These activated agents can thereafter form highly reactive hydroxyl radicals, which depolymerize wood polymers. In this work, cellulose depolymerization by CDH was studied using a model compound, methyl β-D-glucopyranoside. The formation of glucose, arabinose, gluconic acid, erythrulose and formaldehyde were detected and a mechanism for the reaction is proposed. The biological importance of this enzyme-initiated reaction is discussed.
References
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Articles in the same Issue
- Molecular dynamics study of lignin constituents in water
- Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study
- Characterization of active oxygen species under oxygen-alkali bleaching conditions
- Erythro/threo ratio of β-O-4-5 structures as an important structural characteristic of lignin. Part 4: Variation in the erythro/threo ratio in softwood and hardwood lignins and its relation to syringyl/guaiacyl ratio
- Stereoisomers of 3,3′-dimethoxy-4,4′-stilbenequinone
- Liquefaction of CCA-treated wood and elimination of metals from the solvent by precipitation
- Condensed tannins from steamed Acacia mearnsii bark
- Chemical composition and antifungal activity of essential oil isolated from Chamaecyparis formosensis Matsum. wood
- Direct measurement of strain distribution along a wood bond line. Part 1: Shear strain concentration in a lap joint specimen by means of electronic speckle pattern interferometry
- Direct measurement of strain distribution along a wood bond line. Part 2: Effects of adhesive penetration on strain distribution
- Rate-independent mechanical behavior of biaxially stressed wood: Experimental observations and constitutive modeling as an orthotropic two-surface elasto-plastic material
- Characterization of sugar maple wood-polymer composites: Monomer retention and polymer retention
- New insights into wood and cement interaction
- Wood-water sorption isotherm prediction with artificial neural networks: A preliminary study
- Hygroscopicity of resorcinol-formaldehyde resin and aqueous vinyl polymer-isocyanate resin in high humidity
- Modelling anisotropic maturation strains in wood in relation to fibre boundary conditions, microstructure and maturation kinetics
- Properties of chemically and mechanically isolated fibres of spruce (Picea abies [L.] Karst.). Part 3: Mechanical characterisation
- Resistance of thermo-hygro-mechanically densified wood to colonisation and degradation by brown-rot fungi
- Microbial colonizers of freshly sawn yellow-poplar (Liriodendron tulipifera L.) lumber in two seasons. Part 3: Yeasts
- Efficacy of a copper(II)/oxine copper wood preservative mixture after 69 months of outdoor ground-contact exposure and a proposed mechanism to explain the observed synergism
- Differentiation of indoor wood decay fungi with MALDI-TOF mass spectrometry
- Horst H. Nimz celebrates his 75th birthday on April, 21st, 2005
- Erratum
Articles in the same Issue
- Molecular dynamics study of lignin constituents in water
- Oxidative cellulose degradation by cellobiose dehydrogenase from Phanerochaete chrysosporium: A model compound study
- Characterization of active oxygen species under oxygen-alkali bleaching conditions
- Erythro/threo ratio of β-O-4-5 structures as an important structural characteristic of lignin. Part 4: Variation in the erythro/threo ratio in softwood and hardwood lignins and its relation to syringyl/guaiacyl ratio
- Stereoisomers of 3,3′-dimethoxy-4,4′-stilbenequinone
- Liquefaction of CCA-treated wood and elimination of metals from the solvent by precipitation
- Condensed tannins from steamed Acacia mearnsii bark
- Chemical composition and antifungal activity of essential oil isolated from Chamaecyparis formosensis Matsum. wood
- Direct measurement of strain distribution along a wood bond line. Part 1: Shear strain concentration in a lap joint specimen by means of electronic speckle pattern interferometry
- Direct measurement of strain distribution along a wood bond line. Part 2: Effects of adhesive penetration on strain distribution
- Rate-independent mechanical behavior of biaxially stressed wood: Experimental observations and constitutive modeling as an orthotropic two-surface elasto-plastic material
- Characterization of sugar maple wood-polymer composites: Monomer retention and polymer retention
- New insights into wood and cement interaction
- Wood-water sorption isotherm prediction with artificial neural networks: A preliminary study
- Hygroscopicity of resorcinol-formaldehyde resin and aqueous vinyl polymer-isocyanate resin in high humidity
- Modelling anisotropic maturation strains in wood in relation to fibre boundary conditions, microstructure and maturation kinetics
- Properties of chemically and mechanically isolated fibres of spruce (Picea abies [L.] Karst.). Part 3: Mechanical characterisation
- Resistance of thermo-hygro-mechanically densified wood to colonisation and degradation by brown-rot fungi
- Microbial colonizers of freshly sawn yellow-poplar (Liriodendron tulipifera L.) lumber in two seasons. Part 3: Yeasts
- Efficacy of a copper(II)/oxine copper wood preservative mixture after 69 months of outdoor ground-contact exposure and a proposed mechanism to explain the observed synergism
- Differentiation of indoor wood decay fungi with MALDI-TOF mass spectrometry
- Horst H. Nimz celebrates his 75th birthday on April, 21st, 2005
- Erratum