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Influence of hemolysis on troponin testing: studies on Beckman Coulter UniCel Dxl 800 Accu-TnI and overview of the literature
-
Giuseppe Lippi
,
Paola Avanzini
Published/Copyright:
September 6, 2011
Abstract
Background: Hemolyzed specimens are the leading pre-analytical problem in the laboratory practice, and exert a negative impact on test results. We assessed the reliability of Beckman Coulter UniCel Dxl 800 Accu-TnI testing on hemolyzed specimens.
Methods: Twelve non-hemolyzed K2EDTA-anticoagulated samples displaying Accu-TnI values >0.20 μg/L and nine with values <0.04 μg/L were selected and three aliquots were obtained from each. The first (“#A”) was processed without further manipulation, whereas the second (“#B”) and third (“#C”) were hemolyzed by aspirating anticoagulated blood through a thin needle. Plasma was separated and tested for hemolysis index (HI) and Accu-TnI.
Results: As compared with aliquot #A (HI: 0), a progressive increase of hemolysis occurred in aliquots #B (HI: 25) and #C (HI: 45). The concentration of Accu-TnI gradually decreased from aliquots #A (0.89 μg/L, 0.20–20.16 μg/L), to aliquots #B (0.81 μg/L, 95% CI 0.17–18.37 μg/L; p=0.041) and #C (0.78 μg/L, 95% CI 0.15–17.48 μg/L; p=0.026). In 0/12 (aliquots #B) and 3/12 cases (aliquots #C) the percent decrease exceeded 20% variation. The values remained unchanged in nine samples with Accu-TnI <0.04 μg/L.
Conclusions: Accu-TnI values decrease in hemolyzed samples, but the bias might not be clinically significant in samples with hemoglobin <14.5 g/L.
Received: 2011-7-13
Accepted: 2011-8-4
Published Online: 2011-9-6
Published in Print: 2011-12-1
©2011 by Walter de Gruyter Berlin Boston
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Keywords for this article
hemolysis;
hemolyzed specimens;
interference;
preanalytical variability;
troponin
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- Appropriate labelling of blood collection tubes: a step ahead towards patient’s safety
- Reviews
- New insights into adipose tissue dysfunction in insulin resistance
- Role of biomarkers in cardioncology
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