Article
Licensed
Unlicensed
Requires Authentication
Use of a fully automated immunoassay for celiac disease screening in a pediatric population
-
Krista Ress
Published/Copyright:
March 24, 2011
Abstract
Background: Celiac disease (CD) is an immune-mediated disorder of the small bowel in which gluten and related cereal ingestion leads to chronic inflammation and damage to the small bowel mucosa in genetically susceptible individuals. The presence of IgA type tissue-transglutaminase antibodies (IgA-anti-tTG) in individuals without IgA deficiency is a highly-specific indicator of CD. We compared the accuracy of IgA-anti-tTG detection using a fully automated fluoroenzyme immunoassay (FEIA) with that of the standard enzyme-linked immunosorbent assay (ELISA) in a clinical setting.
Methods: We tested 1227 serum samples obtained from three groups of subjects: 1160 randomly selected schoolchildren, 36 pediatric patients with biopsy confirmed CD and 31 control children with biopsy confirmed normal small bowel mucosa. IgA-anti-tTG detection was compared using the FEIA method and the ELISA method, both of which use baculovirus-expressed recombinant human tissue-transglutaminase (tTG) as the antigen.
Results: Both tests identified pediatric patients with CD equally well in the random population (0.34% with CI: 0.09%–0.88%). Furthermore, both tests gave similar results for the CD group and the control group with normal small bowel mucosa (sensitivity: 90% and specificity: 100% for both assays).
Conclusions: The FEIA method is an equivalent substitute for the traditional ELISA for IgA-anti-tTG detection associated with CD in a random pediatric population.
Received: 2010-7-16
Accepted: 2011-1-15
Published Online: 2011-03-24
Published in Print: 2011-06-01
©2011 by Walter de Gruyter Berlin Boston
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editors’ and Publisher’s Note
- CCLM Award for The Most Cited Paper Recently Published
- Reviews
- Some views on proteomics in diabetes
- Cancer biomarkers for hepatocellular carcinomas: from traditional markers to recent topics
- Minireviews
- Low-density lipoprotein receptor-related protein 1: new functions for an old molecule
- Could serum measurements of S100 proteins be reliable markers to predict recurrence in meningiomas?
- Opinion Paper
- Focusing on the clinical impact of standardization of creatinine measurements: a report by the EFCC Working Group on Creatinine Standardization
- General Clinical Chemistry and Laboratory Medicine
- Use of a fully automated immunoassay for celiac disease screening in a pediatric population
- Evaluation of four commercial biuret reagent kits of serum total protein by the American Association for Clinical Chemistry reference measurement procedure
- Renal function parameters during early and late recovery periods following an all-out 21-km run in trained adolescent runners
- Increased urinary neutrophil gelatinase associated lipocalin in urinary tract infections and leukocyturia
- The severity of pseudohyperkalaemia is not dependent upon the stage of chronic kidney disease: a prospective study
- Modified phosphatidylserine-dependent antithrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies
- Perchlorate (Irenat®) may falsely lower measured ionised calcium
- Comparison of thyroglobulin antibody interference in first- and second-generation thyroglobulin immunoassays
- A case of consistent discrepancies between urine and blood human chorionic gonadotropin measurements
- Reference Values and Biological Variations
- Reference change values for monitoring dehydration
- Cancer Diagnostics
- Diagnostic value of pro-gastrin-releasing peptide for small cell lung cancer: a meta-analysis
- Plasma concentrations of angiogenetic factors and angiogenetic inhibitors in patients with ductal pancreatic neoplasms. A pilot study
- Cardiovascular Diseases
- High-sensitivity troponin T improves the prognostic value of N-terminal pro-B-type natriuretic peptide in patients with stable coronary artery disease: results from the LURIC Study
- Letters to the Editor
- Results of a pilot external quality assessment study on free protoporphyrin in erythrocytes
- Trueness assessment of Korean nation-wide glucose proficiency testing
- Genetic polymorphism of cytochrome P450 1A1 in a northern Italian population: high frequency of the CYP1A1*2C allele
- Prestaining of PCR products with SYBR Green for agarose gel electrophoresis: advantages and limitations
- A bias in quantitative RT-PCR limit of detection is induced by the use of cancer cell lines in the molecular detection of circulating tumor cells
- Treatment of hepatitis B virus-associated membranous nephritis patients in Chinese: an open parallel controlled trial
- Evidence on the cause of false positive troponin I results with the Beckman AccuTnI method
- Cross-reactivity of insulin analogs with the Diasorin Liaison Insulin assay
- Improved turnaround time for neonatal hematology profile tests (complete blood count) using a new microcollection tube
- Erratum
- Pharmacogenetics of tacrolimus after renal transplantation: analysis of polymorphisms in genes encoding 16 drug metabolizing enzymes
Keywords for this article
anti-tissue transglutaminase antibodies;
celiac disease;
immunology;
screening
Articles in the same Issue
- Editors’ and Publisher’s Note
- CCLM Award for The Most Cited Paper Recently Published
- Reviews
- Some views on proteomics in diabetes
- Cancer biomarkers for hepatocellular carcinomas: from traditional markers to recent topics
- Minireviews
- Low-density lipoprotein receptor-related protein 1: new functions for an old molecule
- Could serum measurements of S100 proteins be reliable markers to predict recurrence in meningiomas?
- Opinion Paper
- Focusing on the clinical impact of standardization of creatinine measurements: a report by the EFCC Working Group on Creatinine Standardization
- General Clinical Chemistry and Laboratory Medicine
- Use of a fully automated immunoassay for celiac disease screening in a pediatric population
- Evaluation of four commercial biuret reagent kits of serum total protein by the American Association for Clinical Chemistry reference measurement procedure
- Renal function parameters during early and late recovery periods following an all-out 21-km run in trained adolescent runners
- Increased urinary neutrophil gelatinase associated lipocalin in urinary tract infections and leukocyturia
- The severity of pseudohyperkalaemia is not dependent upon the stage of chronic kidney disease: a prospective study
- Modified phosphatidylserine-dependent antithrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies
- Perchlorate (Irenat®) may falsely lower measured ionised calcium
- Comparison of thyroglobulin antibody interference in first- and second-generation thyroglobulin immunoassays
- A case of consistent discrepancies between urine and blood human chorionic gonadotropin measurements
- Reference Values and Biological Variations
- Reference change values for monitoring dehydration
- Cancer Diagnostics
- Diagnostic value of pro-gastrin-releasing peptide for small cell lung cancer: a meta-analysis
- Plasma concentrations of angiogenetic factors and angiogenetic inhibitors in patients with ductal pancreatic neoplasms. A pilot study
- Cardiovascular Diseases
- High-sensitivity troponin T improves the prognostic value of N-terminal pro-B-type natriuretic peptide in patients with stable coronary artery disease: results from the LURIC Study
- Letters to the Editor
- Results of a pilot external quality assessment study on free protoporphyrin in erythrocytes
- Trueness assessment of Korean nation-wide glucose proficiency testing
- Genetic polymorphism of cytochrome P450 1A1 in a northern Italian population: high frequency of the CYP1A1*2C allele
- Prestaining of PCR products with SYBR Green for agarose gel electrophoresis: advantages and limitations
- A bias in quantitative RT-PCR limit of detection is induced by the use of cancer cell lines in the molecular detection of circulating tumor cells
- Treatment of hepatitis B virus-associated membranous nephritis patients in Chinese: an open parallel controlled trial
- Evidence on the cause of false positive troponin I results with the Beckman AccuTnI method
- Cross-reactivity of insulin analogs with the Diasorin Liaison Insulin assay
- Improved turnaround time for neonatal hematology profile tests (complete blood count) using a new microcollection tube
- Erratum
- Pharmacogenetics of tacrolimus after renal transplantation: analysis of polymorphisms in genes encoding 16 drug metabolizing enzymes
