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MMP-9 expression in peripheral blood mononuclear cells and the association with clinicopathological features and prognosis of nasopharyngeal carcinoma
-
Jian-Rong He
,
Cui Cui
Published/Copyright:
January 14, 2011
Abstract
Background: Matrix metalloproteinase-9 (MMP-9) shows significant involvement with cancer invasion and therefore has prognostic significance. Tumor tissues from nasopharyngeal carcinoma (NPC) patients are not easy to access, and peripheral blood mononuclear cells (PBMC) are a valuable alternative. This study compared the expression levels of MMP-9 in PBMCs from NPC patients and healthy controls and evaluated the potential as a prognostic biomarker for NPC patients.
Methods:MMP-9 mRNA concentrations in PBMCs from 146 NPC patients and 110 healthy controls were measured using quantitative real-time PCR. Differences in MMP-9 expression between NPC patients and controls were evaluated using non-parametric tests, and the effects of different clinical characteristics on MMP-9 expression were also analyzed. The Kaplan-Meier test and Cox regression model were used to explore the association between MMP-9 expression and overall survival of NPC patients.
Results:MMP-9 mRNA levels in PBMCs were significantly higher in NPC patients than in healthy controls (p<0.001). Increased MMP-9 expression was associated with the following clinical characteristics: advanced clinical stage (p<0.001), T stage (p=0.016), N stage (p=0.002), histology type (p=0.037), and poor overall survival (p=0.049).
Conclusions:MMP-9 mRNA expression in PBMCs from NPC patients is increased and associated with the clinical characteristics and overall survival. It supports the belief that MMP-9 may contribute to the progression of NPC.
Keywords: matrix metalloproteinase-9; mRNA expression; nasopharyngeal carcinoma; peripheral blood mononuclear cells; prognosis
Received: 2010-8-27
Accepted: 2010-11-2
Published Online: 2011-01-14
Published in Print: 2011-04-01
©2011 by Walter de Gruyter Berlin New York
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Keywords for this article
matrix metalloproteinase-9;
mRNA expression;
nasopharyngeal carcinoma;
peripheral blood mononuclear cells;
prognosis
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