Lack of association between DAZ gene methylation patterns and spermatogenic failure
-
Wei Wu
Abstract
Background: Abnormal DNA methylation of the male germ line is proposed as a possible mechanism causing compromised spermatogenesis in some men diagnosed with idiopathic infertility. Previous studies suggested that aberrant DNA methylation of several genes is associated with disruptions in spermatogenesis. However, little information is available on DNA methylation patterns of testis-specific genes in idiopathic male infertility.
Methods: To investigate the association between DAZ gene methylation patterns and spermatogenic failure, we performed an analysis of methylation patterns in 174 idiopathic infertile patients and 58 fertile controls using bisulfite-modified sequencing.
Results: We found that the methylation patterns of CpG island (CGI) in the DAZ gene promoter region were different between somatic cells and spermatic cells in the control group. DAZ gene methylation patterns among groups with different spermatogenic status were the same in somatic cells, completely methylated. The results were concordant in spermatic cells, completely unmethylated, in groups with different degrees of spermatogenesis, except for the group with azoospermia (AZ).
Conclusions: Our data indicate that the methylation patters of the DAZ gene are not associated with spermatogenic failure. This suggests that epigenetic modification of DAZ is unlikely to be involved in the etiology of spermatogenic failure.
Clin Chem Lab Med 2010;48:355–60.
©2010 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Editorial
- Standardization of assays for clinically important enzymes that have high biologic variation: what is all the fuss about?
- Minireview
- Standardization in clinical enzymology: a challenge for the theory of metrological traceability
- Review
- Laboratory reporting of hemostasis assays: the final post-analytical opportunity to reduce errors of clinical diagnosis in hemostasis?
- Opinion Paper
- The underestimated problem of using serum magnesium measurements to exclude magnesium deficiency in adults; a health warning is needed for “normal” results
- Genetics and Molecular Diagnostics
- Integrated PCR amplification and detection processes on a Lab-on-Chip platform: a new advanced solution for molecular diagnostics
- Matrix metalloproteinase-7 (MMP-7) polymorphism is a risk factor for endometrial cancer susceptibility
- Association of the CD28/CTLA4/ICOS polymorphisms with susceptibility to rheumatoid arthritis
- Lack of association between DAZ gene methylation patterns and spermatogenic failure
- General Clinical Chemistry and Laboratory Medicine
- Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method
- Multicentre evaluation of the Tosoh HbA1c G8 analyser
- Performance of a fully automated quantitative neopterin measurement assay in a routine voluntary blood donation setting
- Assay of oxidized fibrinogen reactivity (OFR) as a biomarker of oxidative stress in human plasma: the role of lysine analogs
- Decrease in uric acid in acute ischemic stroke correlates with stroke severity, evolution and outcome
- Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue
- Is heparin plasma suitable for the determination of B-type natriuretic peptide on the Beckman-Coulter Access 2?
- Composition of kidney stone fragments obtained after extracorporeal shock wave lithotripsy
- Reference Values and Biological Variations
- Gender does not influence cystatin C concentrations in healthy volunteers
- Stability of serum thyroid hormones following 8–11 years of cold storage
- Infectious Diseases
- Detection and quantitation of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples using automated sample preparation and real time PCR
- Letters to the Editor
- The polymorphisms –1131T>C and the S19W of the APOA5 gene are not associated with coronary artery disease in a Brazilian population
- Tissue factor +5466A>G and –1208D>I genetic polymorphisms and severity of rheumatoid arthritis
- Ability of deferasirox to bind iron during measurement of iron
Articles in the same Issue
- Editorial
- Standardization of assays for clinically important enzymes that have high biologic variation: what is all the fuss about?
- Minireview
- Standardization in clinical enzymology: a challenge for the theory of metrological traceability
- Review
- Laboratory reporting of hemostasis assays: the final post-analytical opportunity to reduce errors of clinical diagnosis in hemostasis?
- Opinion Paper
- The underestimated problem of using serum magnesium measurements to exclude magnesium deficiency in adults; a health warning is needed for “normal” results
- Genetics and Molecular Diagnostics
- Integrated PCR amplification and detection processes on a Lab-on-Chip platform: a new advanced solution for molecular diagnostics
- Matrix metalloproteinase-7 (MMP-7) polymorphism is a risk factor for endometrial cancer susceptibility
- Association of the CD28/CTLA4/ICOS polymorphisms with susceptibility to rheumatoid arthritis
- Lack of association between DAZ gene methylation patterns and spermatogenic failure
- General Clinical Chemistry and Laboratory Medicine
- Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method
- Multicentre evaluation of the Tosoh HbA1c G8 analyser
- Performance of a fully automated quantitative neopterin measurement assay in a routine voluntary blood donation setting
- Assay of oxidized fibrinogen reactivity (OFR) as a biomarker of oxidative stress in human plasma: the role of lysine analogs
- Decrease in uric acid in acute ischemic stroke correlates with stroke severity, evolution and outcome
- Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue
- Is heparin plasma suitable for the determination of B-type natriuretic peptide on the Beckman-Coulter Access 2?
- Composition of kidney stone fragments obtained after extracorporeal shock wave lithotripsy
- Reference Values and Biological Variations
- Gender does not influence cystatin C concentrations in healthy volunteers
- Stability of serum thyroid hormones following 8–11 years of cold storage
- Infectious Diseases
- Detection and quantitation of Epstein-Barr virus (EBV) DNA in EDTA whole blood samples using automated sample preparation and real time PCR
- Letters to the Editor
- The polymorphisms –1131T>C and the S19W of the APOA5 gene are not associated with coronary artery disease in a Brazilian population
- Tissue factor +5466A>G and –1208D>I genetic polymorphisms and severity of rheumatoid arthritis
- Ability of deferasirox to bind iron during measurement of iron
