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A high-performance liquid chromatography method for the determination of carbamazepine and carbamazepine-10,11-epoxide and its comparison with chemiluminescent immunoassay
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Carlos Eduardo Leite
Published/Copyright:
March 11, 2009
Abstract
Background: Carbamazepine is a first-choice antiepileptic drug for the treatment of simple and complex partial seizures. The use of an established therapeutic range for carbamazepine concentration is limited by the presence of carbamazepine-10,11-epoxide, its active metabolite that significantly contributes to the efficacy and toxicity and is not routinely measured and accounted for. This article describes the development of a HPLC method for determination of carbamazepine and carbamazepine-10,11-epoxide in serum, and compares it with chemiluminescence immunoassay to evaluate the importance of considering the active metabolite in therapeutic strategies.
Methods: The procedure involves protein precipitation, separation on a reverse-phase column and ultraviolet detection. The analytical procedure proved to be sensitive, selective, precise, accurate and linear (regression coefficients >0.999) in the range of 0.5–25.0 μg/mL and 0.1–10.0 μg/mL for quantification of carbamazepine and carbamazepine-10,11-epoxide, respectively. For the comparison between methods, serum samples of 75 patients using the medication were evaluated.
Results: The Pearson correlation coefficient showed that the carbamazepine concentrations measured by HPLC are significantly higher than those obtained by immunoassay (mean difference of 1.07 μg/mL, 95% limits of agreement from –0.65 to 2.80 μg/mL).
Conclusions: This difference may be decisive for the therapy. In some cases, this may affect the individual dosage adjustment and subsequent treatment.
Clin Chem Lab Med 2009;47:458–63.
Keywords: carbamazepine; carbamazepine-10,11-epoxide; chemiluminescence immunoassay; high-performance liquid chromatography; therapeutic drug monitoring
Received: 2008-9-2
Accepted: 2009-1-9
Published Online: 2009-03-11
Published in Print: 2009-04-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
carbamazepine;
carbamazepine-10,11-epoxide;
chemiluminescence immunoassay;
high-performance liquid chromatography;
therapeutic drug monitoring
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