Chymotrypsin effects on the determination of sperm parameters and seminal biochemistry markers
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Fang Chen
Abstract
Background: Few reports of the effects of treatment with chymotrypsin on the determination of sperm parameters and seminal biochemistry markers are documented.
Methods: Sperm parameters of 63 liquefied and 27 non-liquefied samples, untreated or treated with chymotrypsin, were evaluated using computer-assisted semen analysis. In addition, biochemistry markers such as γ-glutamyltranspeptidase, α-glucosidase and fructose in 50 liquefied and 39 non-liquefied samples, untreated or treated with chymotrypsin, were determined.
Results: Treatment with chymotrypsin had no effect on sperm concentration, motility, motility a and b, straightness, curvilinear velocity, straight line velocity, average path velocity and beat cross frequency in both liquefied and non-liquefied semen. However, linearity (p=0.025) decreased and the amplitude of the lateral head (p=0.029) increased significantly in non-liquefied semen after treatment with chymotrypsin. The levels of γ-glutamyltranspeptidase, α-glucosidase and fructose in seminal plasma were unaffected by chymotrypsin, regardless of liquefaction status.
Conclusions: Chymotrypsin had no effects on the detection of sperm parameters and biochemistry markers, and could be used to treat non-liquefied samples before semen analysis in the andrology laboratory.
Clin Chem Lab Med 2006;44:1335–9.
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©2006 by Walter de Gruyter Berlin New York
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