Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
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Yukiko Fujita
Abstract
The humoral immune responses of 69 active tuberculosis (TB) patients against three major mycobacterial lipid antigens, monoacyl phosphatidylinositol dimannoside (Ac-PIM2), trehalose 6,6′-dimycolate (TDM-T) and trehalose 6-monomycolate (TMM-T) from Mycobacterium bovis BCG Tokyo 172, were examined by ELISA. IgG antibodies from active TB patients were reactive against each of the three lipid antigens (Ac-PIM2, TDM-T and TMM-T), giving positive results of 42.0–59.4%. If tests were combined and an overall positive was scored when any of the three tests was positive, sensitivity was 71.0%, showing better discrimination between patients and normal subjects. Although this value is not satisfactory for the clinical diagnosis of active TB, it is still higher than values for Determinar TBGL (56.5%) and MycoDot (31.9%) test results, both of which are commercially available. IgG antibody responses to particular lipid antigens in an individual patient differed diversely between patients. Positive IgG antibody rates and IgG antibody levels to lipid antigens were mostly paralleled by the amount of mycobacteria excreted and by the severity of pathological lesions (size and cavity formation). Serologically positive responsiveness was shown in 60.0% of tuberculin skin test (TST)-negative TB patients. Furthermore, seropositivity for multiple-antigen ELISA in active TB patients was demonstrated in other possible immune-suppressed cases, such as senile, diabetes mellitus and fulminant TB patients. Therefore, in contrast to tests based on cellular immune responses such as the TST, the humoral immune responses of TB patients against mycobacterial lipid antigens were disease-specific and were shown to be useful for the early diagnosis of active TB disease in conjunction with smear and cultivation tests, even if cellular immune responses are decreased.
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©2005 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region
- Isotretinoin therapy induces DNA oxidative damage
- Structural evaluation of plasma α2-macroglobulin in acute pancreatitis
- Failure of the PAXgene™ Blood RNA System to maintain mRNA stability in whole blood
- Reticulocyte hemoglobin measurement – comparison of two methods in the diagnosis of iron-restricted erythropoiesis
- Physical analysis of ejaculate to evaluate the secretory activity of the seminal vesicles and prostate
- Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma
- Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice
- Plasma levels of 7-hydroxymetabolites of dehydroepiandrosterone in healthy Central European aging men
- Prevalence of abnormal thyroid stimulating hormone and thyroid peroxidase antibody-positive results in a population of pregnant women in the Samara region of the Russian Federation
- Impact of standardized calibration on the inter-assay variation of 14 automated assays for the measurement of creatinine in human serum
- Efficacy of three ELISA measurements of anti-cyclic citrullinated peptide antibodies in the early diagnosis of rheumatoid arthritis
- Mean and variance quality control for multiple correlated levels of replicated control samples
- Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
- Analytical evaluation of the Dade Behring Dimension RxL automated N-Terminal proBNP (NT-proBNP) method and comparison with the Roche Elecsys 2010
- Unusually high alkaline phosphatase due to intestinal isoenzyme in a healthy adult
- Deficient α-galactosidase A activity in plasma but no Fabry disease – a pitfall in diagnosis
- Reportable interval of patient examination results and ISO 15189
