Analysis of candidate genes in Polish families with obesity
-
Malgorzata Malczewska-Malec
Abstract
This study analyzes the relationship between risk factors related to overweight/obesity, insulin resistance, lipid tolerance, hypertension, endothelial function and genetic polymorphisms associated with: i) appetite regulation (leptin, melanocortin-3-receptor (MCR-3), dopamine receptor 2 (D2R)); ii) adipocyte differentiation and insulin sensitivity (peroxisome proliferator-activated receptor-γ2 (PPAR-γ2), tumor necrosis factor-α (TNF-α)); iii) thermogenesis and free fatty acid (FFA) transport/catabolism (uncoupling protein-1 (UCP1), lipoprotein lipase (LPL), β2- and β3-adrenergic receptor (β2AR, β3AR), fatty acid transport protein-1 (FATP-1) and iv) lipoproteins (apoliprotein E (apoE), apo CIII).
The 122 members of 40 obese Caucasian families from southern Poland participated in the study. The genotypes were analyzed by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) or by direct sequencing. Phenotypes related to obesity (body mass index (BMI), fat/lean body mass composition, waist-to-hip ratio (WHR)), fasting lipids, glucose, leptin and insulin, as well as insulin during oral glucose tolerance test (OGTT) (4 points within 2 hours) and during oral lipid tolerance test (OLTT) (5 points within 8 hours) were assessed. The insulin sensitivity indexes: homeostasis model assessment of insulin resistance, whole body insulin sensitivity index, hepatic insulin sensitivity and early secretory response to an oral glucose load (HOMA-IR, ISI-COMP, ISI-HOMA and DELTA) were calculated.
The single gene mutations such as C105 T OB and Pro115 Gln PPAR-γ2 linked to morbid obesity were not detected in our group. A weak correlation between obesity and certain gene polymorphisms was observed. Being overweight (25<BMI≤30 kg/m2) significantly correlated with worse FFA tolerance in male PPAR-γ2 12Pro, LPL-H (G) allele carriers. Insulin resistance was found in female PPAR-γ2 Pro12, TNF-α (−308A) and LPL-H (G) allele carriers. Hypertension linked to the PPAR-γ2 Pro allele carriers was characterized by high leptin output during OLTT.
We conclude that the polymorphisms we investigated were weakly correlated with obesity but significantly modified the risk factors of the metabolic syndrome.
Copyright © 2004 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Beyond cholesterol - inflammatory cytokines, the key mediators in atherosclerosis
- The emerging value of P-selectin as a disease marker
- Analysis of candidate genes in Polish families with obesity
- Detection of the Arg702Trp, Gly908Arg and Leu1007fsinsC polymorphisms of the NOD2/CARD15 gene by real-time PCR with melting curve analysis
- Up-regulation of reactive oxygen species (ROS) and resistance to Fas-mediated apoptosis in the C33A cervical cancer cell line transfected with IL-18 receptor
- Rapid detection of mutations in Wilson disease gene ATP7B by DNA strip technology
- Moderate exercise with a dietary vitamin C and E combination protects against streptozotocin-induced oxidative damage to the blood and improves fetal outcomes in pregnant rats
- Plasma levels of 7-hydroxylated dehydroepiandrosterone (DHEA) metabolites and selected amino-thiols as discriminatory tools of Alzheimer's disease and vascular dementia
- Abnormalities in apolipoprotein and lipid levels in an HIV-infected Brazilian population under different treatment profiles: the relevance of apolipoprotein E genotypes and immunological status
- An International Standard for whole blood folate: evaluation of a lyophilised haemolysate in an international collaborative study
- Population-specific reference values for thyroid hormones on the Abbott ARCHITECT i2000 analyzer
- Amphetamines and 3,4-methylendioxymetamphetamine (MDMA): evaluation of KIMS (kinetic interaction of microparticles in solution) assay at two cut-off levels
- Comparison of sensitivity and specificity of serum poly-C avid ribonuclease activity and C-reactive protein concentration in detection of mild and severe acute pancreatitis
- Emergency department triage of patients with acute chest pain: definition of cardiac troponin I decisional value to manage patients without electrocardiographic evidence of ischemia
- Genetic analysis of glutathione S-transferase A1 and T1 polymorphisms in a Japanese population
- The European Register for Specialists in Clinical Chemistry and Laboratory Medicine: Code ofConduct
- Decision cut-off of the polyethylene glycol precipitation technique in screening for macroprolactinemia on Immulite 2000
- No bias between the first and the new version of RIA for serum holo-transcobalamin by Axis-Shield
- Meetings and Awards
- SIBioC 2004: 36th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, Padua, Italy, 8-11 June 2004
Articles in the same Issue
- Beyond cholesterol - inflammatory cytokines, the key mediators in atherosclerosis
- The emerging value of P-selectin as a disease marker
- Analysis of candidate genes in Polish families with obesity
- Detection of the Arg702Trp, Gly908Arg and Leu1007fsinsC polymorphisms of the NOD2/CARD15 gene by real-time PCR with melting curve analysis
- Up-regulation of reactive oxygen species (ROS) and resistance to Fas-mediated apoptosis in the C33A cervical cancer cell line transfected with IL-18 receptor
- Rapid detection of mutations in Wilson disease gene ATP7B by DNA strip technology
- Moderate exercise with a dietary vitamin C and E combination protects against streptozotocin-induced oxidative damage to the blood and improves fetal outcomes in pregnant rats
- Plasma levels of 7-hydroxylated dehydroepiandrosterone (DHEA) metabolites and selected amino-thiols as discriminatory tools of Alzheimer's disease and vascular dementia
- Abnormalities in apolipoprotein and lipid levels in an HIV-infected Brazilian population under different treatment profiles: the relevance of apolipoprotein E genotypes and immunological status
- An International Standard for whole blood folate: evaluation of a lyophilised haemolysate in an international collaborative study
- Population-specific reference values for thyroid hormones on the Abbott ARCHITECT i2000 analyzer
- Amphetamines and 3,4-methylendioxymetamphetamine (MDMA): evaluation of KIMS (kinetic interaction of microparticles in solution) assay at two cut-off levels
- Comparison of sensitivity and specificity of serum poly-C avid ribonuclease activity and C-reactive protein concentration in detection of mild and severe acute pancreatitis
- Emergency department triage of patients with acute chest pain: definition of cardiac troponin I decisional value to manage patients without electrocardiographic evidence of ischemia
- Genetic analysis of glutathione S-transferase A1 and T1 polymorphisms in a Japanese population
- The European Register for Specialists in Clinical Chemistry and Laboratory Medicine: Code ofConduct
- Decision cut-off of the polyethylene glycol precipitation technique in screening for macroprolactinemia on Immulite 2000
- No bias between the first and the new version of RIA for serum holo-transcobalamin by Axis-Shield
- Meetings and Awards
- SIBioC 2004: 36th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, Padua, Italy, 8-11 June 2004
