High-Speed Detection of the Two Common Paraoxonase Polymorphisms Leu55 → Met and Gln192 → Arg by Real-Time Fluorescence PCR and Melting Curves
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Mariann Harangi
Abstract
Human paraoxonase (PON1) is a calcium-dependent esterase exclusively bound to apolipoprotein A-I and clusterin, containing high-density lipoprotein (HDL) particles that hydrolyzes organophosphates and aryl esters. Several studies have indicated that PON1 can prevent low-density lipoprotein (LDL) oxidation by hydrolyzing lipid peroxides in the lipoprotein, which is the crucial first step for atherogenesis. Therefore it may protect against the development of atherosclerosis. Serum PON1 activity has been shown to be decreased in familiar hypercholesterolemia and in diseases that are associated with accelerated atherogenesis. The PON1 gene has two common coding region polymorphisms, Leu55→Met and Gln192→Arg. Both polymorphisms have been identified as independent risk factors for cardiovascular disease in diabetic and non-diabetic patients. We have established high-speed and easy-to-perform genotyping for the two most significant PON1 gene polymorphisms, employing the LightCycler technology and melting curves. This technique eliminates PCR contamination related to sample handling and does not require digestion of PCR products with restriction enzymes and/or fragment separation on gels.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Fecal Elastase-1 as a Test for Pancreatic Function: a Review
- α2-Macroglobulin Deletion Polymorphism and Plasma Levels in Late Onset Alzheimer's Disease
- High-Speed Detection of the Two Common Paraoxonase Polymorphisms Leu55 → Met and Gln192 → Arg by Real-Time Fluorescence PCR and Melting Curves
- Rapid Detection of Polymorphisms of the Nitric Oxide Cascade
- Salivary Electrolyte Concentrations Are Associated with Cystic Fibrosis Transmembrane Regulator Genotypes
- Granulocyte-Colony Stimulating Factor (G-CSF) and Macrophage-Colony Stimulating Factor (M-CSF) in Colorectal Cancer Patients
- Sialic Acid, Intercellular Adhesion Molecule-1 and Rheumatoid Arthritis: a Study on the Erythrocyte Membrane
- Determination of Organochlorine Pesticide Residues in the Blood of Healthy Individuals
- Reference Values for a Heterogeneous Ferritin Assay and Traceability to the 3rd International Recombinant Standard for Ferritin (NIBSC Code 94/572)
- The Circulating Levels of Cardiac Natriuretic Hormones in Healthy Adults: Effects of Age and Sex
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- Evaluation of Uncertainty of Measurement in Routine Clinical Chemistry - Applications to Determination of the Substance Concentration of Calcium and Glucose in Serum
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- Aspects of the Development of Clinical Chemistry and Laboratory Medicine in the former East Germany
- University Degree in Clinical Laboratory Sciences
- Process Control Reduces the Laboratory Turnaround Time
- Who Needs Two Different Citrate Concentrations in Sample Tubes Used for Coagulation Tests?
- Quantifying the Capability of Detection of Measurement Procedures in Clinical Laboratory Sciences
- Yet another Example of Lack of Laboratory Expertise in Certain Biomedical Publications
- Counterimmunoelectrophoresis: Fast, Easy and Cost-effective Method for the Detection of Autoantibodies to Intracellular Antigens
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