Heterogeneity of DNA Methylation Status Analyzed by Bisulfite-PCR-SSCP and Correlation with Clinico-Pathological Characteristics in Colorectal Cancer
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Masato Maekawa
Abstract
Aberrant DNA methylation has been identified as an important mechanism for inactivation of tumor suppressor genes and mismatch repair genes during carcinogenesis. We used b̲i̲sulfite treatment and the P̲CR s̲ingle strand conformation polymorphism (S̲SCP) (BiPS) technique to analyze methylation status of the promoter regions of the hMLH1, p16, and HIC1 genes in several cancer cell lines and colorectal cancer tissues.
The methylation of the hMLH1, p16 and HIC1 genes was observed in 2, 8, and 13 of 13 cancer cell lines, respectively. The SSCP for p16 and HIC1 in each of the methylation-positive cell lines were similar, indicating relative homogeneity of methylation status and complete methylation in the cell lines. Methylation was observed in 8, 5, and 21 of 25 colorectal cancer tissues for the hMLH1, p16, and HIC1 genes, respectively. The methylated bands revealed by BiPS analysis of the hMLH1 gene were homogeneous, whereas those of the p16 and HIC1 genes were different in each case. The methylation of the promoter region of the HIC1 gene in colorectal cancer was observed most frequently and could serve as a sensitive marker for colorectal cancer. Methylation status of the hMLH1 and p16 gene promoters was correlated with microsatellite instability status, tumor location, and differentiation but not with K-ras mutation or allelic loss of p53.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Special Issues of the Journal
- Clinical Biological and Genetic Heterogeneity of the Inborn Errors of Pulmonary Surfactant Metabolism
- The Effects of Impaired Trace Element Status on Polymorphonuclear Leukocyte Activation in the Development of Vascular Complications in Type 2 Diabetes Mellitus
- Free and Complexed Prostate-Specific Antigen (PSA) in the Early Detection of Prostate Cancer
- Heterogeneity of DNA Methylation Status Analyzed by Bisulfite-PCR-SSCP and Correlation with Clinico-Pathological Characteristics in Colorectal Cancer
- Serum Amyloid A Protein (SAA) in Colorectal Carcinoma
- Metal Exposure from Amalgam Alters the Distribution of Trace Elements in Blood Cells and Plasma
- Diabetic Cataract and the Total Antioxidant Status in Aqueous Humor
- Candidate Gene Polymorphism in Cardiovascular Disease: A Comparative Study of Frequencies between a French and an Italian Population
- Reference Values for Plasma Dipeptidyl Peptidase IV Activity and Their Association with Other Laboratory Parameters
- Influence of Index of Individuality on False Positives in Repeated Sampling from Healthy Individuals
- Regional Reference Values for some Quantities Measured with the ADVIA Centaur Analyser. A Model of Co-operation between the In Vitro Diagnostic Industry and Clinical Laboratories
- Tissue Polypeptide-Specific Antigen (TPS) Concentrations in Cerebrospinal Fluid in Patients with Breast Cancer Metastases in the Central Nervous System
- The Measurement of Anti-Hepatitis C Virus Antibody in Sera Using a New Generation Immunoassay
- Quality Specifications for Cardiac Troponin Assays
- IFCC News January 2001
