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Comparison of Two Different Methods for Measurement of Phenylalanine in Dried Blood Spots
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Adriana Rivero
Published/Copyright:
June 1, 2005
Abstract
Phenylketonuria is an inherited metabolic disorder caused by a defect in the hydroxylation of phenylalanine. Newborn screening is crucial for the diagnosis and treatment of this disease. A phenylalanine dehydrogenase-coupled enzymatic assay (Quantase) in microtiter plates for the screening of phenylketonuria was evaluated and compared with our routine method based on the modified fluorometric McCaman method. The test exhibited a linear calibration curve with a good slope as well as sufficient imprecision (< 10%), recovery (99.23 ± 4.86%) and limit of detection (54.5 μmol/l). One hundred and ninety dried blood spots were analysed by this enzymatic method and compared with McCaman's. Although Quantase (Teknovas, Bilbao, Spain) showed a phenylalanine mean level in dried blood spot 18.2 μmol/l higher than that obtained with our routine method, the agreement between both techniques was considered acceptable.
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Published Online: 2005-06-01
Published in Print: 2000-08-21
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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- Laboratory Support of the Clinical Nutrition Service
- A new Method for Fast Haptoglobin Phenotyping and Hemoglobin Binding Capacity Calculation Based on Capillary Zone Electrophoresis
- Apolipoprotein E Polymorphism and Serum Concentration in Alzheimer's Disease in Nine European Centres: the ApoEurope Study
- Association of the Apolipoprotein B Gene Polymorphisms with Cholesterol Levels and Response to Fluvastatin in Brazilian Individuals with High Risk for Coronary Heart Disease
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