Simple and Broadly Applicable Sample Preparation by Use of Magnetic Glass Particles
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Knut Bartl
Abstract
A key issue in nucleic acid-based assays related to infectious disease testing and genetic testing is the sample preparation. This has to be convenient, efficient and suitable for automation.
We describe a new method based on the use of glass magnetic particles. The steps involved are the lysis of bacterial cells and viral particles by chaotropic salts, binding of released nucleic acids to the magnetic glass particles, the removal of inhibitors and the release of purified nucleic acids ready for PCR amplification. The method fulfills most of the requirements for sample preparation such as sensitivity, universality and suitability for automation.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Human Genomics: The Basis of the Medicine of Tomorrow. Recent Progress in Molecular Biology Technology. First IFCC-Roche Conference – Singapore, 15–18 March 1998
- Does Clinical Chemistry Have a Future?
- The Human Genome Project: From Mapping to Sequencing
- The Human Genome Project and the Role of Genetics in Health Care
- Mutation Detection and Mutation Databases
- Population Genomics: Laying the Groundwork for Genetic Disease Modeling and Targeting
- Oligonucleotide-Based Gene Therapy for Cardiovascular Disease
- Signal Transduction Pathways and Modulation of Gene Activity
- Do Overexpressed Oncoproteins Cause Malignant Growth of Cancer Cells? – Studied by Antisense Oligonucleotides
- Ethical and Legal Issues in the Procurement, Storage and Use of DNA
- DNA Extraction and Stability for Epidemiological Studies
- Simple and Broadly Applicable Sample Preparation by Use of Magnetic Glass Particles
- A Multilocus Genotyping Assay for Cardiovascular Disease
- Optimized Non-Radioactive Protein Truncation Test for Mutation Analysis of the Adenomatous Polyposis Coli (APC) Gene
- Monitoring of Inhibitors of Enzymatic Amplification in Polymerase Chain Reaction and Evaluation of Efficacy of RNA Extraction for the Detection of Hepatitis C Virus Using the Internal Control
- Quantification of Relative Expression of Genes with Homologous Sequences Using Fluorescence-Based Single-Strand Conformation Polymorphism Analysis – Application to Lactate Dehydrogenase and Cyclooxygenase Isozymes
- Automation of Polymerase Chain Reaction-Based Systems for Detection of Hepatitis C Virus RNA
- A Simple PCR with Different 3′ Ends of the Third Primer for Detection of Defined Point Mutations: HCV Genotyping as an Example
- Two Quantitative Methods for Imaging Samples in Molecular Biology
- Restriction Digest PCR (RD-PCR) for the Analysis of Gene Mutations. Application to Ki-ras
- Direct Detection of Mycobacterium tuberculosis in Respiratory Specimens Using an Automated DNA Amplification Assay and a Single Tube Nested Polymerase Chain Reaction (PCR)
- Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay
- Molecular Diagnostics for Cardiovascular Disease
- Association of Apolipoprotein E Polymorphism and Concentration with Serum Lipids and Apo-lipoprotein Level in the Chinese fromShanghai
- Angiotensin Converting Enzyme Gene Polymorphism in Coronary Artery Disease: The Indian Scenario
- Mutation C677T of Methylenetetrahydrofolate Reductase Gene Is Not Associated with Coronary Artery Disease, but Possibly with Albuminuria, in Type 2 Diabetic Patients
- Cholesteryl Ester Transfer Protein Mutations, Protein Activity and HDL-Cholesterol Concentration
- Molecular Biology of Alcohol Dependence, a Complex Polygenic Disorder
- Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR
- The Impact of Molecular Diagnosis on Familial Colorectal Cancer
- Screening for Tumour Suppressor p16(CDKN2A) Germline Mutations in Israeli Melanoma Families
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- Detection of CYP2D6*3 and 2D6*4 Allelic Variants by PCR-Restriction Fragment Length Polymorphism
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