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Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis
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John W. Smalley
Published/Copyright:
August 19, 2008
Abstract
The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively blocks haem dissociation from the protein, prevented haemoglobin breakdown. Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack.
Received: 2008-4-1
Accepted: 2008-4-28
Published Online: 2008-08-19
Published in Print: 2008-09-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
gingipains;
haem;
haemoglobin;
periodontal disease;
Porphyromonas;
protease
Articles in the same Issue
- Review
- Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis
- Protein Structure and Function
- The role of glycosylation and domain interactions in the thermal stability of human angiotensin-converting enzyme
- Fluoride complexes of oncogenic Ras mutants to study the Ras-RasGAP interaction
- Docking of tryptophan analogs to trytophanyl-tRNA synthetase: implications for non-canonical amino acid incorporations
- Molecular mechanisms of aromatase inhibition by new A, D-ring modified steroids
- A common binding motif for various bacteria of the bacteria-binding peptide SRCRP2 of DMBT1/gp-340/salivary agglutinin
- Putative identification of an amphipathic α-helical sequence in hemolysin of Escherichia coli (HlyA) involved in transmembrane pore formation
- Proteolysis
- Homologous and heterologous expression and maturation processing of extracellular glutamyl endopeptidase of Staphylococcus epidermidis
- Cleaved SLPI, a novel biomarker of chymase activity
- Expression of tissue and plasma kallikreins and kinin B1 and B2 receptors in lung cancer
- Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis
- Novel Techniques
- Fast in vitro translation system immobilized on a surface via specific biotinylation of the ribosome
