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Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII

  • Shirley L. Farias , Regiane A. Sabatini , Tatiana C. Sampaio , Izaura Y. Hirata , Maria Helena S. Cezari , Maria A. Juliano , Edward D. Sturrock , Adriana K. Carmona and Luiz Juliano
Published/Copyright: June 1, 2006
Biological Chemistry
From the journal Volume 387 Issue 5

Abstract

Extracellular matrix and soluble plasma proteins generate peptides that regulate biological activities such as cell growth, differentiation and migration. Bradykinin, a peptide released from kininogen by kallikreins, stimulates vasodilatation and endothelial cell proliferation. Various classes of substances can potentiate these biological actions of bradykinin. Among them, the best studied are bradykinin potentiating peptides (BPPs) derived from snake venom, which can also strongly inhibit angiotensin I-converting enzyme (ACE) activity. We identified and synthesized sequences resembling BPPs in the vicinity of potential proteolytic cleavage sites in the collagen XVIII molecule, close to endostatin. These peptides were screened as inhibitors of human recombinant wild-type ACE containing two intact functional domains; two full-length ACE mutants containing only a functional C- or N-domain catalytic site; and human testicular ACE, a natural form of the enzyme that only contains the C-domain. The BPP-like peptides inhibited ACE in the micromolar range and interacted preferentially with the C-domain. The proteolytic activity involved in the release of BPP-like peptides was studied in human serum and human umbilical-vein endothelial cells. The presence of enzymes able to release these peptides in blood led us to speculate on a physiological mechanism for the control of ACE activities.

Keywords: ACE inhibitors; angiogenesis; bradykinin potentiating peptides; endostatin
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Published Online: 2006-06-01
Published in Print: 2006-05-01

©2006 by Walter de Gruyter Berlin New York

Articles in the same Issue

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  3. Functional studies of the small subunit of EcoHK31I DNA methyltransferase
  4. Functional analysis of amino acid residues at the dimerisation interface of KpnI DNA methyltransferase
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  6. Tyr-48, a conserved residue in ribotoxins, is involved in the RNA-degrading activity of α-sarcin
  7. Pathogenicity of catalytic antibodies: catalytic activity of Bence Jones proteins from myeloma patients with renal impairment can elicit cytotoxic effects
  8. Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco
  9. Catalytic pathways of Euphorbia characias peroxidase reacting with hydrogen peroxide
  10. Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system
  11. A spectroscopic analysis of the interaction between the human regulatory proteins RACK1 and Ki-1/57
  12. Subcellular localisation of human inositol 1,4,5-trisphosphate 3-kinase C: species-specific use of alternative export sites for nucleo-cytoplasmic shuttling indicates divergent roles of the catalytic and N-terminal domains
  13. The gating effect of calmodulin and calcium on the connexin50 hemichannel
  14. C-Terminal fusion of eGFP to the bradykinin B2 receptor strongly affects down-regulation but not receptor internalization or signaling
  15. Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII
  16. μ-Calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation
  17. Cathepsin L splice variants in human breast cell lines
https://doi.org/10.1515/BC.2006.078
Keywords for this article
ACE inhibitors; angiogenesis; bradykinin potentiating peptides; endostatin

Articles in the same Issue

  1. Protein aggregation in crowded environments
  2. Nitrite, a naturally occurring precursor of nitric oxide that acts like a ‘prodrug’
  3. Functional studies of the small subunit of EcoHK31I DNA methyltransferase
  4. Functional analysis of amino acid residues at the dimerisation interface of KpnI DNA methyltransferase
  5. Conformation and stability of the Streptococcus pyogenes pSM19035-encoded site-specific β recombinase, and identification of a folding intermediate
  6. Tyr-48, a conserved residue in ribotoxins, is involved in the RNA-degrading activity of α-sarcin
  7. Pathogenicity of catalytic antibodies: catalytic activity of Bence Jones proteins from myeloma patients with renal impairment can elicit cytotoxic effects
  8. Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco
  9. Catalytic pathways of Euphorbia characias peroxidase reacting with hydrogen peroxide
  10. Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system
  11. A spectroscopic analysis of the interaction between the human regulatory proteins RACK1 and Ki-1/57
  12. Subcellular localisation of human inositol 1,4,5-trisphosphate 3-kinase C: species-specific use of alternative export sites for nucleo-cytoplasmic shuttling indicates divergent roles of the catalytic and N-terminal domains
  13. The gating effect of calmodulin and calcium on the connexin50 hemichannel
  14. C-Terminal fusion of eGFP to the bradykinin B2 receptor strongly affects down-regulation but not receptor internalization or signaling
  15. Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII
  16. μ-Calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation
  17. Cathepsin L splice variants in human breast cell lines
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