Home Recombinant expression, purification and cross-reactivity of chenopod profilin: rChe a 2 as a good marker for profilin sensitization
Article
Licensed
Unlicensed Requires Authentication

Recombinant expression, purification and cross-reactivity of chenopod profilin: rChe a 2 as a good marker for profilin sensitization

  • Rodrigo Barderas , Mayte Villalba and Rosalía Rodríguez
Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 385 Issue 8

Abstract

Chenopod pollen is one of the major sources of allergens in some locations in the US, southern Europe and desert countries, and pollen profilin (Che a 2) is a major allergen. Recombinant Che a 2 (rChe a 2) has been produced in Escherichia coli cells with a final yield of 25 mg/l of cell culture. The expressed protein was isolated and structurally characterized by means of mass spectrometry, Edman degradation and circular dichroism. rChe a 2 displayed a molecular mass of 13 959 Da, which agrees with that of the amino acid sequence. The N-terminal amino acid sequence indicated the correct processing of the recombinant product. The immunological analysis of rChe a 2 showed IgG- and IgE-binding capabilities equivalent to those of its natural counterpart, Che a 2, isolated from the pollen. Inhibition experiments showed high cross-reactivity degrees with different allergenic sources. Inhibition degrees of > 95% and > 80% were obtained for chenopod profilin and, respectively, latex and pollen extracts, whereas 10–95% of inhibition was observed for different plant-derived foods. Due to its close relation to other allergenic profilins from pollens, plant-derived foods and latex, rChe a 2 could be a useful tool in clinical trials to detect profilin-allergic patients and perhaps, depending on its clinical relevance, in specific immunotherapy of these hypersensitive individuals.

:

Corresponding author e-mail:

References

Asturias, J.A., Arilla, M.C., Gómez-Bayon, N., Martínez, J., Martínez, A., and Palacios, R. (1997). Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. J. Allergy Clin. Immunol.100, 365–372.10.1016/S0091-6749(97)70250-1Search in Google Scholar

Barderas, R., Villalba, M., Lombardero, M., and Rodríguez, R.(2002). Identification and characterization of Che a 1 allergen from Chenopodium album pollen. Int. Arch. Allergy Immunol.127, 47–54.10.1159/000048168Search in Google Scholar PubMed

Barderas, R., Villalba, M., Batanero, E., Pascual, C.Y., and Rodríguez, R. (2003). The role of profilin and polcalcin in chenopod pollen allergy. J. Allergy Clin. Immunol.111,1132–1133.10.1067/mai.2003.1445Search in Google Scholar PubMed

Barderas, R., Villalba, M., Pascual, C.Y., Batanero, E., and Rodríguez, R. (2004). Profilin (Che a 2) and polcalcin (Che a 3)are relevant allergens of Chenopodium album pollen. Isolation, amino acid sequences and immunological properties. J.Allergy Clin. Immunol.113, 1192–1198.Search in Google Scholar

Caballero, T., and Martín-Esteban, M. (1998). Association between pollen hypersensitivity and edible vegetable allergy: a review. J. Invest. Allergol. Clin. Immunol.8, 6–16.Search in Google Scholar

Callejo, A., Sanchís, M.E., Armentia, A., Moneoa, I., and Fernández, A. (2002). A new pollen-fruit cross-reactivity. Allergy57, 1088–1089.10.1034/j.1398-9995.2002.23836_10.xSearch in Google Scholar PubMed

Daschner, A., Crespo, J.F., and Pascual, C.Y. (1998). Specific IgE to recombinant vegetal panallergen (rBet v 2) and fruit allergy in pollinic patients. Allergy53, 614–618.10.1111/j.1398-9995.1998.tb03938.xSearch in Google Scholar PubMed

Díaz-Perales, A., Lombardero, M., Sánchez-Monge, R., García-Sellés, F.J., Pernas, M., Fernández-Rivas, M., Barber, D., and Salcedo, G. (2000). Lipid-transfer proteins as potential plant panallergens: cross-reactivity among proteins of Artemisia pollen, Castanea nut and Rosaceae fruits, with different IgE-binding capacities. Clin. Exp. Allergy30, 1403–1410.10.1046/j.1365-2222.2000.00909.xSearch in Google Scholar PubMed

Fedorov, A.A., Ball, T., Valenta, R., and Almo, S.C. (1997). X-ray crystal structures of birch pollen profilin and Phl p 2. Int. Arch. Allergy Immunol.113, 109–113.10.1159/000237520Search in Google Scholar PubMed

Feo, F., Martínez, J., Martínez, A., Galindo, P.A., Cruz, A., Garcia, R., Guerra, F., and Palacios, R. (1997). Occupational allergy in saffron workers. Allergy52, 633–641.10.1111/j.1398-9995.1997.tb01042.xSearch in Google Scholar PubMed

Fernández, J., Asturias, J.A., and Martínez, A. (2000). Biological standardization and allergen characterization of date palm pollen (Phoenix dactylifera). Allergy55, 140.Search in Google Scholar

Florido, J.F., Quiralte, J., Saénz De San Pedro, B., and Bartolomé, B. (2000). Cross-reactivity between Olea europaea and Chenopodium album pollens. Allergy55, 158.Search in Google Scholar

Ledesma, A., Rodríguez, R., and Villalba, M. (1998a). Olive-pollen profilin. Molecular and immunologic properties. Allergy53, 520–526.10.1111/j.1398-9995.1998.tb04090.xSearch in Google Scholar PubMed

Ledesma, A., Villalba, M., Batanero, E., and Rodríguez, R. (1998b). Molecular cloning and expression of active Ole e 3, a major allergen from olive-tree pollen and member of a novel family of Ca2+-binding proteins (polcalcins) involved in allergy. Eur. J. Biochem.258, 454–459.10.1046/j.1432-1327.1998.2580454.xSearch in Google Scholar PubMed

Lombardero, M., Duffort, O., Selles, J.G., Hernández, J., and Carreira, J. (1985). Cross-reactivity among Chenopodiaceae and Amaranthaceae. Ann. Allergy.54, 430–436.Search in Google Scholar

Lombardero, M., Duffort, O., and Carreira, J. (1991). Allergenic significance of chenopod pollen. In: Allergenic pollen and pollinosis in Europe, G. D’Amato, F.T.M. Spieksma and S. Bonini, eds. (Oxford, UK: Blackwell Scientific Publishers), pp. 128–131.Search in Google Scholar

Lowry, O., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275.10.1016/S0021-9258(19)52451-6Search in Google Scholar

Rihs, H.P., Chen, Z., Rozynek, P., Baur, X., Lundberg, M., and Cremer, R. (2000). PCR-based cloning, isolation, and IgE-binding properties of recombinant latex profilin (rHev b 8). Allergy55, 712–717.10.1034/j.1398-9995.2000.00553.xSearch in Google Scholar

Rihs, H.P., Chen, Z., Rueff, F., Petersen, A., Rozynek, P., Heimann, H., and Baur, X. (1999). IgE binding of the recombinant allergen soybean profilin (rGly m 3) is mediated by conformational epitopes. J. Allergy Clin. Immunol.104, 1293–1301.10.1016/S0091-6749(99)70027-8Search in Google Scholar

Rodríguez-Pérez, R., Fernández-Rivas, M., González-Mancebo, E., Sánchez-Monge, R., Díaz-Perales, A., and Salcedo, G. (2003). Peach profilin: cloning, heterologous expression and cross-reactivity with Bet v 2. Allergy58, 635–640.10.1034/j.1398-9995.2003.00198.xSearch in Google Scholar

Scheurer, S., Wangorsch, A., Nerkamp, J., Skov, P.S., Ballmer-Weber, B., Wuthrich, B. et al. (2001). Cross-reactivity within the profilin panallergen family investigated by comparison of recombinant profilins from pear (Pyr c 4), cherry (Pru av 4) and celery (Api g 4) with birch pollen profilin Bet v 2. J. Chromatogr. B. Biomed. Sci. Appl.756, 315–325.10.1016/S0378-4347(01)00090-1Search in Google Scholar

Valenta, R., Dûchene, M., Vrtala, S., Birkner, T., Ebner, C., Hirschwehr, R., Breitenbach, M., Rumpold, H., Scheiner, O., and Kraft, D. (1991). Recombinant allergens for immunoblot diagnosis of tree-pollen allergy. J. Allergy Clin. Immunol.88, 889–894.10.1016/0091-6749(91)90245-JSearch in Google Scholar

Valenta, R., Duchene, M., Ebner, C., Valent, P., Sillaber, C., Deviller, P., Ferreira, F., Tejkl, M., Edelman, H., and Kraft, D. (1992). Profilins constitute a novel family of functional plant panallergens. J. Exp. Med.175, 377–385.10.1084/jem.175.2.377Search in Google Scholar PubMed PubMed Central

Vallverdú, A., García-Ortega, P., Martínez, J., Martínez, A., Esteban, M.I., de Molina, M., Fernández, J., Bartolomé, B., and Palacios, R. (1997). Mercurialis annua: characterization of main allergens and cross-reactivity with other species. Int. Arch. Allergy Immunol.112, 356–364.10.1159/000237480Search in Google Scholar PubMed

Van Ree, R., Fernández-Rivas, M., Cuevas, M., Van Wijngaarden, M., and Aalberse, Rc. (1995). Pollen-related allergy to peach and apple: an important role for profilin. J. Allergy Clin. Immunol.95, 726–734.Search in Google Scholar

Vieths, S., Scheurer, S., and Ballmer-Weber, B. (2002). Current understanding of cross-reactivity of food allergens and pollen. Ann. NY Acad. Sci.964, 47–68.10.1111/j.1749-6632.2002.tb04132.xSearch in Google Scholar PubMed

Wagner, S., and Breiteneder, H. (2002). The latex-fruit syndrome. Biochem. Soc. Trans.30, 935–940.10.1042/bst0300935Search in Google Scholar PubMed

Wensing, M., Akkerdaas, J.H., van Leeuwen, W.A., Stapel, S.O., Bruijnzeel-Koomen, C.A., Aalberse, R.C., Bast, B.J., Knulst, A.C., and van Ree, R. (2002). IgE to Bet v 1 and profilin: cross-reactivity patterns and clinical relevance. J. Allergy Clin. Immunol.110, 435–442.10.1067/mai.2002.126380Search in Google Scholar PubMed

Wopfner, N., Willeroidee, M., Hebenstreit, D., van Ree, R., Aalbers, M., Briza, P., Thalhamer, J., Ebner, C., Richter, K., and Ferreira, F. (2002). Molecular and immunological characterization of profilin from mugwort pollen. Biol. Chem.383, 1779–1789.10.1515/BC.2002.199Search in Google Scholar PubMed

Wurtzen, P.A., Nelson, H.S., Lowenstein, H., and Ipsen, H. (1995). Characterization of Chenopodiales (Amaranthus retroflexus, Chenopodium album, Kochia scoparia, Salsola pestifer) pollen allergens. Allergy50, 489–497.10.1111/j.1398-9995.1995.tb01184.xSearch in Google Scholar PubMed

Published Online: 2005-06-01
Published in Print: 2004-08-01

© Walter de Gruyter

Downloaded on 29.11.2025 from https://www.degruyterbrill.com/document/doi/10.1515/BC.2004.089/html?lang=en
Scroll to top button