Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells
-
S. Bősze
Abstract
Interleukin-6 (IL-6) is a helical cytokine exerting pleiotropic activities including the regulation of hematopoiesis, B cell activation and acute-phase reaction. The structure-function relationship of the molecule is the subject of intensive investigation using point and deletion mutants. Our objective was to analyse the role of the N-terminal 18–46 region in IL-6-mediated expression of junB protooncogene and fibrinogen production, reflecting the acute phase response, with synthetic overlapping peptides. mRNA expression of junB was monitored by competitive RT-PCR, while sandwich ELISA was used for the detection of fibrinogen in the supernatant of HepG2 human hepatoma cells. We found that even short synthetic octapeptides can be stimulatory (in the absence of IL-6) or inhibitory (in the presence of IL-6) in both assays. To establish the molecular mechanism by which synthetic peptides exert their biological effects electromobility shift assay was carried out using HepG2 nuclear extracts. Peptides inducing junB expression initiate gel shifts of STAT3/DNA complexes, which may indicate the involvement of this signal transduction pathway. Circular dicroism spectroscopy data suggest that 8-11-mer peptides representing different parts of the 18–46 region have a marked tendency to adopt ordered conformations in a water/trifluoroethanol (1:1 v/v) mixture. Competition studies with rhIL-6 and selected fluorophorelabelled peptides indicate the presence of more than one binding site on soluble IL-6 receptor. Considering the possible multiple etiologic role of IL-6 in the pathogenesis of various diseases, these peptides could be useful for dissection of IL-6 related biological effects.
Copyright © 2003 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Roumen Tsanev, Pioneer of the Early Days of Nucleic Acid Gel Electrophoresis and Histone Code Epigenetics
- Recombinant RNase Z Does Not Recognize CCA as Part of the tRNA and Its Cleavage Efficieny Is Influenced by Acceptor Stem Length
- HIV-1 TAR as Anchoring Site for Optimized Catalytic RNAs
- Transcription of Different Exons 1 of the Human Neuronal Nitric Oxide Synthase Gene Is Dynamically Regulated in a Cell- and Stimulus- Specific Manner
- Hepatitis B Virus Core Antigen: Enhancement of Its Production in Escherichia coli, and Interaction of the Core Particles with the Viral Surface Antigen
- Determinants of Enzymatic Specificity in the Cys-Met-Metabolism PLP-Dependent Enzyme Family: Crystal Structure of Cystathionine γ-Lyase from Yeast and Intrafamiliar Structure Comparison
- In vitro Uptake and Stability Study of pVEC and Its All-D Analog
- Calpastatin Exon 1B-Derived Peptide, a Selective Inhibitor of Calpain: Enhancing Cell Permeability by Conjugation with Penetratin
- Structure-Activity Relationships of Glucose-Dependent Insulinotropic Polypeptide (GIP)
- Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells
- Induction of Apoptosis in Vascular Cells by Plasminogen Activator Inhibitor-1 and High Molecular Weight Kininogen Correlates with Their Anti-Adhesive Properties
- The Role of Octadecanoids and Functional Mimics in Soybean Defense Responses
- Invasiveness of Transformed Human Breast Epithelial Cell Lines Is Related to Cathepsin B and Inhibited by Cysteine Proteinase Inhibitors
- Retinoids Inhibit Human Epidermal Keratinocyte RNase P Activity
- A Putative Glutathione Peroxidase of Drosophila Encodes a Thioredoxin Peroxidase That Provides Resistance against Oxidative Stress But Fails to Complement a Lack of Catalase Activity
- Characterization of Calmodulin Binding to the Orphan Nuclear Receptor ERRγ
- Evidence for Coordinated Induction and Repression of Ecto-5'-Nucleotidase (CD73) and the A2a Adenosine Receptor in a Human B Cell Line
- Bauhinia Proteinase Inhibitor-Based Synthetic Fluorogenic Substrates for Enzymes Isolated from Insect Midgut and Caterpillar Bristles
- Synthesis and Characterization of EMPO-Derived 5,5-Disubstituted 1-Pyrroline N-Oxides as Spin Traps Forming Exceptionally Stable Superoxide Spin Adducts
Articles in the same Issue
- Roumen Tsanev, Pioneer of the Early Days of Nucleic Acid Gel Electrophoresis and Histone Code Epigenetics
- Recombinant RNase Z Does Not Recognize CCA as Part of the tRNA and Its Cleavage Efficieny Is Influenced by Acceptor Stem Length
- HIV-1 TAR as Anchoring Site for Optimized Catalytic RNAs
- Transcription of Different Exons 1 of the Human Neuronal Nitric Oxide Synthase Gene Is Dynamically Regulated in a Cell- and Stimulus- Specific Manner
- Hepatitis B Virus Core Antigen: Enhancement of Its Production in Escherichia coli, and Interaction of the Core Particles with the Viral Surface Antigen
- Determinants of Enzymatic Specificity in the Cys-Met-Metabolism PLP-Dependent Enzyme Family: Crystal Structure of Cystathionine γ-Lyase from Yeast and Intrafamiliar Structure Comparison
- In vitro Uptake and Stability Study of pVEC and Its All-D Analog
- Calpastatin Exon 1B-Derived Peptide, a Selective Inhibitor of Calpain: Enhancing Cell Permeability by Conjugation with Penetratin
- Structure-Activity Relationships of Glucose-Dependent Insulinotropic Polypeptide (GIP)
- Interleukin-6 N-Terminal Peptides Modulate the Expression of junB Protooncogene and the Production of Fibrinogen in HepG2 Cells
- Induction of Apoptosis in Vascular Cells by Plasminogen Activator Inhibitor-1 and High Molecular Weight Kininogen Correlates with Their Anti-Adhesive Properties
- The Role of Octadecanoids and Functional Mimics in Soybean Defense Responses
- Invasiveness of Transformed Human Breast Epithelial Cell Lines Is Related to Cathepsin B and Inhibited by Cysteine Proteinase Inhibitors
- Retinoids Inhibit Human Epidermal Keratinocyte RNase P Activity
- A Putative Glutathione Peroxidase of Drosophila Encodes a Thioredoxin Peroxidase That Provides Resistance against Oxidative Stress But Fails to Complement a Lack of Catalase Activity
- Characterization of Calmodulin Binding to the Orphan Nuclear Receptor ERRγ
- Evidence for Coordinated Induction and Repression of Ecto-5'-Nucleotidase (CD73) and the A2a Adenosine Receptor in a Human B Cell Line
- Bauhinia Proteinase Inhibitor-Based Synthetic Fluorogenic Substrates for Enzymes Isolated from Insect Midgut and Caterpillar Bristles
- Synthesis and Characterization of EMPO-Derived 5,5-Disubstituted 1-Pyrroline N-Oxides as Spin Traps Forming Exceptionally Stable Superoxide Spin Adducts
