PAF67, a Novel Protein That Is Associated with the Initiation-Competent Form of RNA Polymerase I
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Peter Seither
Abstract
Mammalian RNA polymerase I (Pol I) is a multisubunit enzyme that is decorated with accessory proteins, termed PAFs (polymeraseassociated factors). The presence or absence of distinct PAFs may account for the functional differences of distinct fractions of cellular Pol I, and suggests that PAFs could be targets of regulatory pathways. Here we describe and functionally characterize PAF67, a novel 67 kDa protein that is tightly associated with a subpopulation of cellular Pol I. Both PAF67-containing and deficient Pol I transcribe nonspecific templates with similar efficiency, however, only the enzyme that contains PAF67 is capable of specifically transcribing rDNA templates. PAF67 colocalizes with Pol I in the nucleolus at sites of active rDNA transcription, indicating that PAF67 serves a role in rDNA transcription initiation. The results suggest that association of PAF67 with the core enzyme endows Pol I with the capability to assemble into a productive transcription initiation complex at the rDNA promoter.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Roger D. Kornberg Felix Hoppe-Seyler Lecturer 2001
- The Eukaryotic Gene Transcription Machinery
- Paper of the Year 2000: Award to Eva Estébanez-Perpiñá and Pablo Fuentes-Prior
- GABAC Receptors: A Molecular View
- Recent Advances in Plant MAP Kinase Signalling
- The Chemical Biology of Ras Lipidation
- This Is the End: Processing, Editing and Repair at the tRNA 3-Terminus
- Binding of IRE-BP to Its Cognate RNA Sequence: SFM Studies on a Universal RNA Backbone for the Analysis of RNA-Protein Interaction
- PAF67, a Novel Protein That Is Associated with the Initiation-Competent Form of RNA Polymerase I
- Characterization of RNase P Holoenzymes from Methanococcus jannaschii and Methanothermobacter thermoautotrophicus
- Identification of Functional Regions of Guanylate Cyclase-Activating Protein 1 (GCAP1) Using GCAP1/GCIP Chimeras
- Cooperativity of Binding Epitopes and Receptor Chains in the BMP/TGFß Superfamily
- Cyclo19,31[D-Cys19]-uPA19-31 Is a Potent Competitive Antagonist of the Interaction of Urokinase-Type Plasminogen Activator with Its Receptor (CD87)
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- Biophysical Characterization of Lipopolysaccharide and Lipid A Inactivation by Lactoferrin
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- Functional Dissection of Trigger Factor and DnaK: Interactions with Nascent Polypeptides and Thermally Denatured Proteins
- A Persulfurated Cysteine Promotes Active Site Reactivity in Azotobacter vinelandii Rhodanese
- The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene Localisation
- A Structural Role for Asp83 in the Photoactivation of Rhodopsin
- Photo-CIDNP 13C Magic Angle Spinning NMR on Bacterial Reaction Centres: Exploring the Electronic Structure of the Special Pair and Its Surroundings
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