Article
Licensed
Unlicensed
Requires Authentication
Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells
-
A.C. Verschuur
Published/Copyright:
June 1, 2005
Abstract
Cytidine triphosphate (CTP) synthase is one of the key enzymes in pyrimidine nucleotide anabolic pathways. The activity of this enzyme is elevated in various malignancies including acute lymphocytic leukemia (ALL). In this study we investigated the activity of CTP synthase in various human blood cells isolated from healthy volunteers by density centrifugation and elutriation centrifugation. We also investigated the mRNA expression of CTP synthase in lymphocytes and monocytes. The highest activity of CTP synthase was found in thrombocytes (6.48 nmol CTP × mg−1 × h−1), followed by that of monocytes (2.23), lymphocytes (1.69), granulocytes (0.52) and erythrocytes (0.42). The activity of CTP synthase in whole blood samples was at an intermediate level (1.27). The mRNA expression of CTP synthase in monocytes was comparable to that observed in lymphocytes.
Published Online: 2005-6-1
Published in Print: 1999-1-4
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- To Our Authors, Readers and Subscribers. Biological Chemistry 1999 - Increased Page Number and Online Accessibility
- Proto-Oncogenes, Unlike Harmless Genes, Tend to Be Dispersed in the Human Genome: Selection Against Out-of-Register Recombination?
- The Human H2A and H2B Histone Gene Complement
- Efficient Control of raf Gene Expression by CAP and Two Raf Repressors that Bend DNA in Opposite Directions
- Molecular Models of Acidic PePtides From Pea Bud Chromatin And Seminal Plasma. Divalent Cations-Mediated Interaction With Dna
- Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells
- Studies with Lysine N6-Hydroxylase. Effect of a Mutation in the Assumed FAD Binding Site on Coenzyme Affinities and on Lysine Hydroxylating Activity
- The Recombinant Thermosome from the Hyperthermophilic Archaeon Methanopyrus kandleri: In Vitro Analysis of Its Chaperone Activity
- Not More than Three Tissue Kallikreins Identified from Organs of the Guinea Pig
- Activation of Protein C by Arginine-Specific Cysteine Proteinases (Gingipains-R) from Porphyromonas gingivalis
- Alteration of Mosaic Methylation of the Repeat Unit of the Human Ribosomal RNA Genes in Lung Cancer
- Degradation of the Cyclic AMP Antagonist Prostaglandylinositol Cyclic Phosphate (Cyclic PIP) by Dephosphorylation
- Homo-Dimeric Spherulin 3a: A Single-Domain Member of the bg-Crystallin Superfamily
- cDNA Sequencing of Guinea Pig a2-HS Glycoprotein, Its Expression in Various Tissues and Acute Phase Expression
- Phage Display Selection of P1 Mutants of BPTI Directed against Five Different Serine Proteinases
Articles in the same Issue
- To Our Authors, Readers and Subscribers. Biological Chemistry 1999 - Increased Page Number and Online Accessibility
- Proto-Oncogenes, Unlike Harmless Genes, Tend to Be Dispersed in the Human Genome: Selection Against Out-of-Register Recombination?
- The Human H2A and H2B Histone Gene Complement
- Efficient Control of raf Gene Expression by CAP and Two Raf Repressors that Bend DNA in Opposite Directions
- Molecular Models of Acidic PePtides From Pea Bud Chromatin And Seminal Plasma. Divalent Cations-Mediated Interaction With Dna
- Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells
- Studies with Lysine N6-Hydroxylase. Effect of a Mutation in the Assumed FAD Binding Site on Coenzyme Affinities and on Lysine Hydroxylating Activity
- The Recombinant Thermosome from the Hyperthermophilic Archaeon Methanopyrus kandleri: In Vitro Analysis of Its Chaperone Activity
- Not More than Three Tissue Kallikreins Identified from Organs of the Guinea Pig
- Activation of Protein C by Arginine-Specific Cysteine Proteinases (Gingipains-R) from Porphyromonas gingivalis
- Alteration of Mosaic Methylation of the Repeat Unit of the Human Ribosomal RNA Genes in Lung Cancer
- Degradation of the Cyclic AMP Antagonist Prostaglandylinositol Cyclic Phosphate (Cyclic PIP) by Dephosphorylation
- Homo-Dimeric Spherulin 3a: A Single-Domain Member of the bg-Crystallin Superfamily
- cDNA Sequencing of Guinea Pig a2-HS Glycoprotein, Its Expression in Various Tissues and Acute Phase Expression
- Phage Display Selection of P1 Mutants of BPTI Directed against Five Different Serine Proteinases
