COMPARISON OF ACTIVITY AND PROTEIN PROFILES OF ENDOTOXINS EXTRACTED AFTER VARIOUS INTERVALS OF BACTERIAL GROWTH ON SOLID MEDIUM

Chapters in this book

1. Frontmatter I
2. PREFACE V
3. CONTENTS VII
4. SECTION I. PLENARY LECTURE
5. EVOLUTION OF IDEAS IN ELECTROPHORETIC DEVELOPMENTS - Selected Highlights 3
6. SECTION II. THEORY AND METHODS
7. A UNIFIED MATHEMATICAL THEORY OF ELECTROPHORETIC PROCESSES 51
8. ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. I: PRINCIPLE AND METHODOLOGY 61
9. ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. II: APPLICATION TO HEMOGLOBIN ANALYSIS 75
10. COMPUTER SIMULATION OF MODEL ISOELECTRIC FOCUSING EXPERIMENTS 83
11. QUANTITATIVE MODELS FOR THE MIGRATION OF ELECTROLYTE GRADIENTS IN AN ELECTRIC FIELD 91
12. APPLICABILITY OF THE CORRELATION LOG SIZE - SQUARE ROOT OF MIGRATION DISTANCE TO SDS ELECTROPHORESIS IN LINEAR POLYACRYLAMIDE GRADIENT GELS 103
13. VOLTAGE REGULATION ACROSS MULTIPLE GEL TUBES IN ELECIROFOCUSING, USING WEAKLY ACIDIC AND BASIC ANOLYTES AND CATHOLYTES 109
14. A GENERAL METHOD FOR THE STUDY OF SOME PROPERTIES OF POLYMERIC PROTEINS 121
15. HIGHLY CROSS-LINKED GRADIENT GELS FOR DETERMINING MOLECULAR WEIGHT WITH NATIVE PROTEINS OF ALL SIZES 127
16. HIGH RESOLUTION DNA SEQUENCING SYSTEM 137
17. POLYMERIZATION KINETICS OF POLYACRYLAMIDE GELS: EFFECTS OF DIFFERENT CROSSLINKERS, TEMPERATURE AND CATALYSTS 147
18. ISOELECTRIC FOCUSING OF IMMUNOGLOBULINS 157
19. SYNTHETIC AMIDE-TYPE OLIGOMERS AS POTENTIAL CARRIER AMPHOLYTES 169
20. A NEW POLYACRYLAMIDE GEL FORMULATION ALLOWING OVEN DRYING OF HIGH PERCENTAGE SLAB GELS ON GELBONÖ® PAG 177
21. RECOVERY OF PROTEINS FROM GEL SLABS BY A COMBINATION OF DISPLACEMENT ELECTROPHORESIS AND ISOELECTRIC FOCUSING 183
22. AUTOMATIC EVALUATION OF ONE-DIMENSIONAL ELECTROPHEROGRAMS BY REFINED MODELLING 189
23. QUANTITATIVE EVALUATION OF CAF - PATTERN BY VIDEO DENSITOMETRY 197
24. IDENTIFICATION OF FUNCTIONAL PROPERTIES ASSOCIATED WITH INDIVIDUAL PROTEINS BY COMPUTER-BASED PATTERN ANALYSIS 207
25. EVALUATION OF ISOELECTRIC FOCUSING PATTERN BY VIDEO DENSITOMETRY 215
26. EFFECTIVE FIXATION AND RAPID STAINING OF PROTEINS IN SDS-PAGE 225
27. FAST VISUALISATION OF PROTEIN BANDS BY IMPREGNATION IN POTASSIUM PERMANGANATE AND SILVER NITRATE 235
28. SILVER STAIN IMMUNOFIXATION FOR a2HS-GLYC0PR0TEIN: A NEW METHOD FOR DETECTION OF PROTEIN HETEROGENEITY 243
29. DEMONSTRATION OF OLIGOCLONAL IGG IN THE UNCONGENTRATED CSF BY SILVER STAIN 249
30. AFFINITY ELECTROPHORETIC SYSTEMS COMPARED WITH THE CORRESPONDING AFFINITY COLUMN SYSTEMS - GENERAL PRINCIPLES 261
31. DEMONSTRATION AND QUANTIFICATION OF MICROHETEROGENEITY FORKS. LECTIN AFFINITY ELECTROPHORESIS OF HUMAN ɑ-FETOPROTEIN 271
32. STUDIES ON HETEROGENEITY OF ANTI-DNP ANTIBODY BY MEANS OF TWO DIMENSIONAL AFFINITY ELECTROPHORESIS 277
33. DETECTION OF ALLERGENS FOLLOWING GEL ELECTROPHORESIS 285
34. QUALITATIVE CHARACTERISTICS OF CAPILLARY ISOTACHOPHORESIS 293
35. FREE FLOW FIELD STEP FOCUSING - A NEW METHOD FOR PREPARATIVE PROTEIN ISOLATION 303
36. ASECS: ANTIGEN-SPECIFIC ELECTROPHORETIC CELL SEPARATION 313
37. PREPARATIVE DENSITY GRADIENT ELECTROPHORESIS OF CELLS AND CELL ORGANELLES. A NEW SEPARATION CHAMBER 317
38. SECTION III. TWO-DIMENSIONAL SEPARATIONS
39. MOLECULAR PROBES FOR HUMAN GENETIC DISEASES BY TWO-DIMENSIONAL PROTEIN ELECTROPHORESIS AND SILVER STAINING 327
40. TWO-DIMENSIONAL ELECTROPHORESIS OF PROTEINS: USE OF CELLULOSE ACETATE MEMBRANE AND ISOELECTRIC FOCUSING GEL 343
41. ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. Ill: APPLICATION TO TWODIMENSIONAL SEPARATIONS 353
42. TWO-DIMENSIONAL ELECTROPHORESIS IN AGAROSE GELS 363
43. ASPECTS OF HIGH RESOLUTION TWO DIMENSIONAL GEL ELECTROPHORESIS : ATTEMPTS TO INCREASE RESOLUTION 371
44. 2D-ELECTROPHORESIS OF PROTEINS IN UNICELLULAR ALGA E. GRACILIS AND YEAST S. UVARUM 381
45. POLYPEPTIDE COMPOSITION OF DIFFERENT CHLOROPLAST FRACTIONS FRON BARLEY ANALYZED BY 1- AND 2-DIMENSIONAL GEL ELECTROPHORESIS 393
46. TWO-DIMENSIONAL ELECTROPHORESIS OF SIMIAN VIRUS 40 (SV40) TUMOR ANTIGEN TWO-DIMENSIONAL ELECTROPHORESIS OF SIMIAN VIRUS 40 (SV40) TUMOR ANTIGEN 403
47. TWO-DIMENSIONAL ELECTROPHORESIS OF FIBRINOGEN AND FIBRIN SUBUNIT CHAINS 413
48. NUMERICAL TAXONOMY OF TWO-DIMENSIONAL PROTEIN MAPS: A RATIONAL BIOCHEMICAL APPROACH TO TUMOR CHARACTERIZATION 423
49. ISO-DALT ANALYSIS OF URINARY PROTEINS IN RENAL FAILURE 435
50. BINDING OF ANTICANCER DIBROMO-DULCITOL AND DIANHYDRO-DULCITOL (GALACTITOL) TO NUCLEAR PROTEINS IN ASCITES TUMOUR CELLS 445
51. A SIMPLE ONE DIMENSIONAL GEL PROCEDURE FOR DETERMINATION OF THE EXTENT OF PHOSPHORYLATION OF RIBOSOMAL PROTEIN S6 453
52. SECTION IV. BIOLOGICAL AND BIOMEDICAL APPLICATIONS
53. COMPARISON OF ACTIVITY AND PROTEIN PROFILES OF ENDOTOXINS EXTRACTED AFTER VARIOUS INTERVALS OF BACTERIAL GROWTH ON SOLID MEDIUM 463
54. POLYACRYLAMIDE GEL ELECTROFOCUSING OF BETA-LACTAMASES FROM PROTEUS MIRABILIS 477
55. POLYACRYLAMIDE-STARCH GEL ELECTROFOCUSING OF CATALASE FROM PSEUDOMONAS AERUGINOSA 481
56. FRACTIONATION OF 7,8-DIHYDROPTEROATE-SYNTHETASE FROM E.COLI BY AFFINITY CHROMATOGRAPHY AND ISOELECTRIC FOCUSING TECHNIQUE 485
57. PURIFICATION OF 7,8-DIHYDROPTERIDINEALCOHOL PYROPHOSPHOKINASE FROM E.COLI AND CHARACTERIZATION BY ULTRATHIN LAYER IEF 491
58. ANALYSIS OF PROTEIN AND POLY-A⁺ RNA IN NORMAL AND SULFURSTARVED SYNECHOCOCCUS 6301 497
59. ELECTROPHORETIC MEASUREMENTS Ql\l PURPLE MEMBRANE PARTICLES 507
60. THE ACTIVITIES OF ISOLATED ALKALINE- AND ACID-DNases ON DENATURED AND NATIVE DNA 511
61. A FUNGAL PROTEINASE RESISTANT PROTEIN IN BOVINE PLASMA 519
62. IDENTIFICATION OF FILARIAL PROTEINS, INDUCING AN IMMUNE RESPONSE IN NATURAL INFECTIONS OF RODENTS WITH DIPETALONEMA VITEAE (FILAROIDEA) BY THE "WESTERN BLOTTING" TECHNIQUE 523
63. AN ECONOMICAL MICROSCALE COUNTER-IMMUNOELECTROPHORESIS METHOD FOR SCREENING OF ALEUTIAN DISEASE IN MINK 535
64. CROSSED IMMUNOELECTROPHORESIS FROM SDS-POLYACRYLAMIDE GELS 545
65. COMPARISON OF WAGTAIL LIVER ISOZYME PATTERNS IN AGAROSE THIN LAYER ISOELECTRIC FOCUSING WITH THOSE OBTAINED BY ISOELECTRIC FOCUSING ON POLYACRYLAMIDE GELS (PAGIF) 551
66. MALATE DEHYDROGENASE ISOENZYMES FROM RABBIT LENS, IRIS AND RETINA 559
67. THE MICROHETEROGENEITY FORMS OF HUMAN SERUM PROTEINS ARE REMARKABLY CONSTANT. A SYSTEMATIC STUDY OF REACTION WITH ConA DURING ELECTROPHORESIS 567
68. AMPLIFICATION OF IMMUNOPRECIPITATES WITH HORSERADISH PEROXIDASE-LABELED PROTEIN A: APPLICATION TO STUDIES OF INTERACTION BETWEEN α-PETROPROTEIN AND LECTINS BY AFFINITY ELECTROPHORESIS 577
69. ANALYSIS OF CENTRIFUGATION GRADIENTS BY FUSED ROCKET IMMUNOELECTROPHORESIS 587
70. QUANTITATIVE IMMUNOELECTROPHORESIS FOR COMPARATIVE ANALYSIS OF HYDROPHOBIC INTRINSIC MEMBRANE PROTEINS 599
71. SENSITIVE MEASUREMENT OF 3H-GLUCOCORTICOID-RECEPTORCOMPLEXES BY GEL-ELECTROPHORESIS 607
72. ELECTROPHORESIS AND ISOELECTRIC FOCUSING OF MAMMALIAN SEMEN PROTEINS 615
73. POLYPEPTIDES SECRETED BY RESIDENT, INFLAMMATORY AND ACTIVATED MACROPHAGES 621
74. DIRECT TISSUE ISOELECTRIC FOCUSING OF NERVOUS SYSTEM AND MUSCLE SECTIONS FOR DETECTION OF IgG PATTERNS 629
75. DETERMINATION OF PREHbA_1c BY USE OF ISOELECTRIC FOCUSING 633
76. ELECTROPHORETIC ANALYSIS OF TISSUES IN DUCHENNE MUSCULAR DYSTROPHY 641
77. CHANGES OF MYOSIN ISOENZYMES IN THE CARDIOMYOPATHIC SYRIAN HAMSTER AND IN THE HUMAN MYOCARDIUM 651
78. CHROMATOFOCUSING OF HIGH DENSITY LIPOPROTEINS 655
79. ISOELECTRIC FOCUSING OF PLASMA LIPOPROTEINS PRE-STAINED WITH TETRAZOLIUM BLUE: A PROMISING DIAGNOSTIC TOOL 665
80. A NEW DETAILED LIPIDOGRAM: METHODS AND CLINICAL APPLICATIONS 675
81. THE COURSE OF PROTEINURIA IN GENTAMICIN-TREATED RATS: IEF AND IMMUNOFLUORESCENCE MARKING OF PROTEINS ON THE PAG-PLATE 683
82. AGAROSE ISOELECTRIC FOCUSING OF UNCONCENTRATED CSF AND RADIOIMMUNOFIXATION FOR DETECTION OF OLIGOCLONAL BANDS IN PATIENTS WITH MULTIPLE SCLEROSIS AND OTHER NEUROLOGICAL DISEASES 691
83. PREPARATIVE ISOELECTRIC FOCUSING IN AGAROSE GELS AND ITS APPLICATION IN THE INVESTIGATION OF GAMMOPATHIES 697
84. ROCKET IMMUNOSELECTION USING PEROXIDASE LABELED ANTIBODIES IN THE DIAGNOSIS OF ALPHA HEAVY CHAIN DISEASE 705
85. CLASSICAL ELECTROPHORETIC TECHNIQUES VERSUS ULTRATHINLAYER FOCUSING AS TOOLS IN POPULATION GENETIC STUDIES 711
86. C4 PHENOTYPES IN CAUCASIANS FROM THE SOUTHEASTERN UNITED STATES 715
87. ISOELECTRIC FOCUSING OF SEED PROTEINS FROM NATURAL GREEK POPULATIONS OF TWO AGROPYRON SPECIES 725
88. PLACENTAL ENZYME POLYMORPHISMS IN THE HUMAN POPULATION OF NORTHERN GREECE 733
89. ELASTASE INHIBITION BY SUBTYPES OF THE M GENETIC VARIANT OF α₁-ANTITRYPSIN 743
90. ISOELECTRIC POINTS AND TITRATION CURVES OF THE THREE HAPTOGLOBIN PEPTIDE CHAINS α,α₂ AND β 751
91. ANTITHROMBIN III: A NEW POLYMORPHISM REVEALED BY ISOELECTROFOCUSING AND IMMUNOFIXATION 761
92. COAGULATION FACTOR XIII B (FXIIIB) ALLOTYPING BY IMMUNOFIXATION ELECTROPHORESIS: CONFIRMATION OF THE THREE-ALLELE-MODEL 767
93. SEPARATION OF LEUKEMIC B CELLS FROM PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA BY DENSITY GRADIENT ELECTROPHORESIS. FUNCTIONAL AND PHENOTYPIC HETEROGENEITY OF THE SEPARATED CELL FRACTIONS 777
94. ELECTROPHORETIC MOBILITY TEST BY STIMULATED LYMPHOCYTE SUPERNATANTS 783
95. SECTION V: ROUND-TABLE DISCUSSIONS
96. A. ACCURACY AND PRECISION OF 2-DIMENSIONAL MACROMOLECULAR MAPPING 797
97. B. A SILVER STAIN WORKSHOP 825
98. C. PEPTIDE FOCUSING 843
99. AUTHOR INDEX 853
100. SUBJECT INDEX 857