FUNCTIONAL DISSECTION OF THE VITAMIN D RECEPTOR TRANSDUCTION PATHWAY

Chapters in this book

1. Frontmatter I
2. FOREWORD V
3. OFFICIAL SPONSORS EIGHTH WORKSHOP ON VITAMIN D IX
4. Contents XI
5. Gene Regulation and Vitamin D
6. A TALE OF TWO GENES: REGULATION OF RAT OSTEOCALCIN AND CHICKEN CALBINDIN-D_26K: BY THE VITAMIN D HORMONE AND ITS PHOSPHORYLATED NUCLEAR RECEPTOR 3
7. VITAMIN D REGULATION OF THE BONE SPECIFIC OSTEOCALCIN GENE IS FUNCTIONALLY RELATED TO OSTEOBLAST GROWTH AND DIFFERENTIATION 12
8. VITAMIN D RESPONSE ELEMENT IN THE RAT OSTEOCALCIN GENE 21
9. KINETICS OF TRH AND 1,25 DIHYDROXYVITAMIN D3 (1,25.D3) INTERACTION ON THE EXPRESSION OF PRL AND GH GENES IN GH3.B6 PITUITARY CELLS 28
10. THE mRNA EXPRESSION OF THE 1,25-DIHYDROXYVITAMIN D₃ (l,25(OH)₂D₃ RECEPTOR (VDR) AND THE C-MYC PROTOONCOGENE IN CULTURED HUMAN KERATINOCYTES 30
11. SEQUENCE SPECIFIC BINDING OF VITAMIN D RECEPTOR TO CANDIDATE 1,25-DIHYDROXYVITAMIN D₃ RESPONSIVE ELEMENTS OF THE CHICK CALBINDIN D_28K GENE PROMOTER 32
12. IDENTIFICATION OF SEQUENCES IN THE HUMAN PARATHYROID HORMONE GENE THAT BIND THE 1,25-DIHYDROXY VITAMIN D3 RECEPTOR 34
13. IDENTIFICATION OF SEQUENCE ELEMENTS IN THE MOUSE CALBINDIN-D28k GENE WHICH CONFER BASAL ACTIVATION AND HORMONE INDUCIBLE RESPONSE 36
14. CHANGES OF 1,25(OH)₂D₃ RECEPTOR EXPRESSION IN DIFFERENT STAGES OF GROWTH PLATE CHONDROCYTES MATURATION 38
15. STUDIES ON THE FUNCTIONAL ANALYSIS OF THE AVIAN CALBINDIND_28K PROMOTER 40
16. SPECIFIC SUPPRESSION OF A 45-KILODALTON MITOCHONDRIAL MEMBRANE PROTEIN BY 1,25-DIHYDROXYVITAMIN D₃ 42
17. 1,25(OH)₂D₃ ADMINISTRATION INDUCES CALMODULIN BINDING PROTEINS IN THE RAT KIDNEY 44
18. EFFECTS OF 1,25-DIHYDROXYCHOLECALCIFEROL AND CALCIUM ON CALCITONIN mRNA LEVELS IN SUCKLING RATS 46
19. 1,25-DIHYDROXYVITAMIN D3 (1,25(OH) ₂D₃) RECEPTOR BINDS TO SPECIFIC SITES ON THE BOVINE PARATHYROID HORMONE (PTH) GENE 48
20. EXPRESSION OF FUNCTIONAL VITAMIN D3 RECEPTOR IN E. COLI 50
21. Receptors for l,25(OH)₂D₃
22. OSTEOCALCIN GENE EXPRESSION AS A MODEL FOR THE GENOMIC ACTIONS OF THE VITAMIN D HORMONE 55
23. MODELLING THE HORMONE-BINDING DOMAIN OF STEROID RECEPTORS INCLUDING VITAMIN D₃ RECEPTOR 64
24. REGULATION OF 1,25-DIHYDROXYVITAMIN D3 RECEPTOR GENE EXPRESSION IN THE PARATHYROID GLAND 75
25. FUNCTIONAL DISSECTION OF THE VITAMIN D RECEPTOR TRANSDUCTION PATHWAY 82
26. PRODUCTION OF ANTIBODIES TO SYNTHETIC PEPTIDES DERIVED FROM THE HUMAN 1,25-DIHYDROXYVITAMIN D₃ RECEPTOR 86
27. CHARACTERIZATION OF VITAMIN D RESISTANCE IN NEW WORLD PRIMATES UTILIZING ESTABLISHED CELL LINES 88
28. CHANGES IN EXPRESSION OF 1,25 (OH)₂D₃ RECEPTOR (VDR) AND C-MYC mRNA IN MYELOID CELL DIFFERENTIATION 90
29. INCREASED l,25(OH)2D3 RECEPTOR LEVEL IN THE INTESTINE AND PARATHYROID OF THE SUBTOTALLY NEPHRECTOMIZED RAT 92
30. A NOVEL MUTATION IN THE STEROID BINDING REGION OF THE VITAMIN RECEPTOR (VDR) GENE IN HEREDITARY VITAMIN RESISTANT RICKETS (HVDRR) 94
31. STUDIES ON 1,25-DIHYDROXYVITAMIN D RECEPTOR GENE IN HUMAN VITAMIN D-RESISTANT RICKETS 96
32. CELL DIFFERENTIATION VS. CELL PROLIFERATION: A STUDY IN HL-60 CELLS OF 1,25(OH)₂D₃ ACTIONS TO EFFECT CHANGES IN THE mRNA FOR C-MYC AND THE 1,25(OH)₂D₃ RECEPTOR 98
33. INCREASED 1,25-DIHYDROXYVITAMIN D3 RECEPTORS IN PERIPHERAL BLOOD MONONUCLEAR CELLS FROM PATIENTS WITH PRIMARY HYPERPARATHYROIDISM 100
34. RECEPTORS FOR CALCITRIOL IN RAT THYROID FOLLICULAR CELLS 102
35. ELEVATED 1,25(OH)₂D₃ RECEPTORS IN SKIN OF ATHYMIC NUDE MICE 104
36. ARE THERE TWO CLASSES OF 1,25-DIHYDROXYVITAMIN D RECEPTORS? 106
37. COMPARATIVE STUDIES ON THE SPECIFICITY OF THE DUODENAL 1,25-DIHYDROXYVITAMIN D3 RECEPTOR IN PIGS AND GOATS 108
38. EFFECT OF GLUCOCORTICOIDS AND 1,25-DIHYDROXYVITAMIN D₃ ON THE DEVELOPMENTAL EXPRESSION OF THE RAT INTESTINAL VITAMIN D RECEPTOR GENE 110
39. OLIGOMETRIC STRUCUTRE OF THE 1.25(OH)₂ VIT D RECEPTOR 112
40. ORGAN DISTRIBUTION OF A HIGH AFFINITY RECEPTOR FOR l,25(OH)₂ VITAMIN D₃ IN THE ATLANTIC COD (GADUS MORHUA), A MARINE TELEOST 114
41. VITAMIN D RECEPTOR MUTATIONS AND HEREDITARY 1,25-DIHYDROXYVITAMIN D RESISTANT RICKETS 116
42. INTERACTION OF THE VITAMIN D RECEPTOR WITH NATURAL AND PALINDROMIC VITAMIN D RESPONSIVE ELEMENTS 125
43. VARIATION IN CONCENTRATION OF BINDING SITES FOR 25 HYDROXYVITAMIN D(250HD) IN KIDNEY RAT TREATED WITH PHENOBARBITAL (PB) AND STEROIDS (ST) 127
44. Structure/Function Studies of Vitamin D Steroids
45. BIOLOGICAL ACTIVITY OF 1,25-DIHYDROXYVITAMIN D ANALOGS: INFLUENCE OF RELATIVE AFFINITIES FOR DBP AND VITAMIN D RECEPTOR 131
46. THE 16-ENE ANALOGS OF 1,25-DIHYDROXYCHOLECALCIFEROL. SYNTHESIS AND BIOLOGICAL ACTIVITY 139
47. STRUCTURE-FUNCTION RELATIONSHIPS IN THE VITAMIN D ENDOCRINE SYSTEM FOR l,25(OH)₂D₃ ANALOGS 146
48. DIFFERENTIAL EFFECTS OF l,25-(OH)₂D₃ AND 22-OXACALCITRIOL (OCT) ON CALCIUM AND PO₄ METABOLISM 155
49. NONGENOMIC ACTIONS OF l,25(OH)₂D₃ IN RAT OSTEOSARCOMA CELLS AND CHICK INTESTINAL EPITHELIAL CELLS: STRUCTURE FUNCTION STUDIES OF LIGAND AGONIST ACTIONS 157
50. SYNTHESIS AND BIOLOGICAL ACTIVITY OF 1 a-HYDROXYLATED VITAMIN D₃ ANALOGUES WITH HYDROXYLATED SIDE CHAINS, MULTI-HOMOLOGATED IN THE 24- OR 24,26,27-POSITIONS 159
51. SYNTHESIS AND BIOLOGICAL ACTIVITY OF 22-OXA VITAMIN D ANALOGUES 161
52. THE 20-EPI MODIFICATION IN THE VITAMIN D SERIES: SELECTIVE ENHANCEMENT OF "NON-CLASSICAL" RECEPTOR-MEDIATED EFFECTS 163
53. AROCALCIFEROLS: A NEW CLASS OF SIDE-CHAIN ANALOGS OF l,25(OH)₂D₃ 165
54. Chemistry of Vitamin D
55. SIDE-CHAIN AND DEUTERIUM LABELED ANALOGS OF 1 a,25-DIHYDROXYVITAMIN D₃ 169
56. SYNTHESIS OF SIDE CHAIN ANALOGS OF VITAMIN D 178
57. CALCULATION OF THE SIDE CHAIN FLEXIBILITY FOR VITAMIN D ANALOGS 186
58. DESIGN AND SYNTHESIS OF NOVEL FLUORESCENCE-LABELING REAGENT FOR VITAMIN D. 188
59. ON CONFORMATIONAL FLEXIBILITY OF VITAMIN D 190
60. SYNTHESIS AND BIOLOGICAL ACTIVITY OF 1a-HYDROXYLATED VITAMIN D ANALOGUES WITH POLY-UNSATURATED SIDE CHAINS 192
61. A SHORT, FLEXIBLE ROUTE TO VITAMIN D METABOLITES AND THEIR SIDE CHAIN ANALOGS 194
62. WAVELENGTH AND TEMPERATURE EFFECTS ON THE PHOTOCHEMISTRY OF VITAMIN D. 196
63. DESIGN AND ASYMMETRIC TOTAL SYNTHESIS OF RING-A ANALOGS OF 1,25- DIHYDROXYVITAMIN D₃ 198
64. RECENT ADVANCES IN THE CHEMISTRY AND SYNTHESIS OF VITAMIN D METABOLITES AND ANALOGUES 199
65. SYNTHESIS OF SIDE CHAIN HOMOLOGATED ANALOGUES OF 1, 25-DIHYDROXYCHOIiECALCIFEROL AND 1,25—DIHYDROXYERGOCAI1CIFEROL 208
66. TOTAL SYNTHESIS OF (C-ll)-SUBSTITUTED ANALOGUES OF la,25-DIHYDROXY-VITAMIN D₃ 210
67. EFFICIENT SYNTHESIS OF 25-HYDROXY-VITAMIN D, AND ITS 26,27- HOMOLOGUES 212
68. Vitamin D Metabolism and Catabolism
69. METABOLISM OF 1,25-DIHYDROXYVITAMIN D3 AND 26,26,26,27,27,27-HEXAFLUORO- 1,25-DIHYttOCiVrmMIN D-, IN HL-60 CELLS 217
70. NO EVIDENCE FOR EXTRARENAL PRODUCTION OF 1,25-DIHYDROXYVITAMIN D IN VITAMIN D-DEFICIENT RATS WITH BCG-INDUCED GRANULOMAS 219
71. EVALUATION OF VITAMIN D BIOAVAILABILITY IN SHEEP WITH RESPECT TO THEIR NUTRITIONAL AND ENVIRONMENTAL CONDITIONS 221
72. CATABOLISM OF la, 2 5-DIHYDROXYDIHYDROTACHYSTEROLj IN VITRO : COMPARISON WITH IN VIVO GENERATED METABOLITES OF DIHYDROTACHYSTEROL3 223
73. PHARMACOKINETICS OF CALCIPOTRIOL AND l,25(OH)₂D₃ IN RATS 225
74. Vitamin D Hydoxylases: Biochemistry and Regulation
75. THE CELLULAR AND MOLECULAR REGULATION OF l,25(OH)2D3 PRODUCTION 229
76. PSEUDO-VITAMIN D DEFICIENCY RICKETS 238
77. PURIFICATION AND CLONING OF CYTOCHROME P450s INVOLVED IN CALCIUM HOMEOSTASIS 246
78. STUDIES ON THE CYTOCHROMES P-450 CATALYZING la-, 24- AND 26- HYDROXYLATIONS OF 25-HYDROXYVITAMIN D₃ IN PIG 255
79. PURIFICATION OF 25-HYDROXYVITAMIN D₃ 24-HYDROXYLASE FROM RAT KIDNEY MITOCHONDRIA 257
80. CHARACTERIZATION OF THE LIVER MITOCHONDRIAL CYTOCHROME P-450 CATALYZING THE 25-HYDROXYLATION OF VITAMIN D₃ 259
81. CA⁺²-CALMODULIN-ACTIVATED PROTEIN KINASE IN STRUCTURALLY INTACT KIDNEY MITOCHONDRIA PHOSPHORYLATES THE ENDOGENOUS FERREDOXIN: MODULATION OF la- AND 24-HYDROXYLASES OF 25-HYDROXYVTTAMIN D₃ 261
82. THE HUMAN RENAL 25-HYDROXYVITAMIN D₃-1 a-HYDROXYLASE: PROPERTIES STUDIED BY ISOTOPE- DILUTION MASS SPECTROMETRY 263
83. SUBCELLULAR LOCALIZATION OF THE 25-HYDROXYVITAMIN D-1- HYDROXYLATION REACTION IN THE AVIAN MYELOMONOCYTIC CELL LINE, HD-11 265
84. 24-HYDROXYLATION OF 1a-OH-D₂ BY CULTURED LIVER CELLS 267
85. PCR CLONING OF THE CHICK KIDNEY FERREDOXIN cDNA AND EFFECT OF VITAMIN D STATUS ON FERREDOXIN mRNA LEVELS 269
86. EFFECT OF LONG-TERM CULTURE, 1aX,25(OH)₂D₃, AND LIPOPOLYSACCHARIDE ON 25(OH)D₃ METABOLISM BY BLOOD DERIVED MONOCYTE-MACROPHAGES FROM A PATIENT WITH CHRONIOC MYELOMONOCYTIC LEUKAEMIA (CML). 271
87. NORMAL 24-HYDROXYLATION OF VITAMIN D METABOLITES IN VITAMIN D DEPENDENCY RICKETS TYPE I: STRUCTURAL IMPLICATIONS FOR THE VITAMIN D HYDROXYLASES 273
88. ACTIVATION OF PROTEIN KINASE C MODULATES DIHYDROXYCHOLECALCIFEROL SYNTHESIS IN RAT RENAL PROXIMAL TUBULES 275
89. UNIQUE PROPERTY OF LIVER MITOCHONDRIAL P450 TO CATALYZE THE TWO PHYSIOLOGICALLY IMPORTANT REACTIONS INVOLVED IN BOTH VITAMIN D ACTIVATION AND CHOLESTEROL CATABOLISM 277
90. PROTEIN KINASE C DEPENDENT DEPHOSPHORYLATION OF A 12.5 KDa RENAL MITOCHONDRIAL PROTEIN IS CORRELATED WITH ALTERED 25(OH)D3 METABOLISM 279
91. GROWTH ZONE CHONDROCYTES AND RESTING ZONE CHONDROCYTES PRODUCE 1,25-(OH)₂D₃ AND 24,25-(OH)2D3 IN VITRO 281
92. AUALINE PHOSPHATASE ACTIVITY IN MITOCHONDRIAL MEMBRANE OF LLC-PK1 CELLS. A POSSIBLE ROLE IN THE l,25(OH)₂D₃ SYNTHESIS 283
93. ACUTE EFFECT OF PHOSPHORUS ON 1-a-HYDROXYLASE ACTIVITY IN THE ISOLATED PERFUSED RAT KIDNEY 285
94. Biological Actions of Other Vitamin D Metabolites
95. CHARACTERISTIC OF TWO NOVEL VITAMIN D. ANALOGUES; 22-OXA- 1 a,25-DIHYDROXYVITAMIN D3 [OCT] AND 2β-³(3-HYDROXYPROPOXY)- 1a,25-DIHYDROXYVITAMIN D₃₃[ED-71] 289
96. SECALCIFEROL ATTENUATES THE CALCAEMIC RESPONSE TO ORAL CALCITRIOL IN CHRONIC RENAL FAILURE 298
97. SERUM CONCENTRATIONS OF la, 25(OH)₂D₃26,23-LACTONE IN NORMAL ADULTS 300
98. 23(S)25(R)-125(OH)₂D₃26,23-LACTONE , A NATURALLY OCCURRING METABOLITE OF 1,25(OH)₂D₃, INHIBITS OSTEOCLAST-LIKE CELL FORMATION IN HUMAN BONE MARROW AND CORD BLOOD CULTURES 302
99. 24R/2 5-DIHYDROXYVITAMIN D3 ACTIVATES OSTEOCALCIN GENE TRANSCRIPTION THROUGH THE VITAMIN D RECEPTOR 304
100. PARATHYROID HORMONE GENE EXPRESSION: IDENTIFICATION OF AN UPSTREAM ELEMENT INVOLVED IN DOWN-REGULATION BY VITAMIN D3 306
101. EVIDENCE FOR A PHYSIOLOGICAL FUNCTION OF 1,25-(OH)2D3 IN TYPE II PNEUMOCYTES FROM FETAL RAT LUNG 308
102. IN VIVO INDUCTION OF RENAL ALKALINE PHOSPHATASE AND OAMMA GLUTAMYL TRANSPEPTIDASE ACTIVITIES BY 24R,25-DIHYDR0XYVITAMIN D3 IN INTACT RATS 310
103. A NOVEL VITAMIN D, ANALOGUE, 22-0XACALCITRI0L(OCT):ITS DIFFERENT BEHAVIOURS FROM CALCITRIOL IN PLASMA TRANSPORT SYSTEM 312
104. COMPARISON OF 1,25 (OH)₂D₃ LEVELS AFTER THE ADMINISTRATION OF CALCITRIOL AND ALFACALCIDOL 314
105. THE ACTIVITY OF 22-OXACALCITRIOL IN OSTEOBLAST-LKE (ROS 17/2.8) CELLS 316
106. CHOLECALCIFEROL METABOLITES INHIBIT INOSITOL POLYPHOSPHATE PRODUCTION IN CLONAL RAT PITUITARY CELLS (GH_d4C₁, CELLS) 318
107. THE EFFECT OF MC 903 AND 26,27 -HEXAFLUORO-1,25-(0H)2D3 ON VITAMIN D METABOLISM AND BONE RESORPTION 320
108. Hormone Secretion and Vitamin D
109. EARLY EFFECTS OF 1,25 DIHYDROXYVITAMIN D3 ON TRH - INDUCED TSH AND GH SECRETION IN THE RAT 325
110. RESPONSIVENESS OF RAT AND MOUSE BONE CELLS TO THE SOMATOSTATIN ANALOGUE SMS 201-995 327
111. SHORT-TERM EFFECTS OF ANDROGEN DEFICIENCY AND HORMONAL SUBSTITUTION ON SERUM CALCIUM HOMEOSTASIS, VITAMIN D, NcAMP AND SERUM OSTEOCALCIN IN AGED MALE RATS 329
112. LACK OF EFFECT OF 1,25 (OH)₂ D₃ ADMINISTRATION ON GLUCOSE TOLERANCE AND INSULIN SECRETION IN NORMAL MAN 333
113. IMPAIRED PARATHYROID HORMONE SECRETION IN PRIMARY HYPOMAGNESEMIA 335
114. MECHANISM OF ACTION OF 1,25-DIHYDROXYVITAMIN D3 ON ISLETS INSULIN SECRETION FROM VITAMIN D3 DEFICIENT RATS 337
115. Intestinal and Renal Transport of Calcium and Phosphorus
116. KETOCONAZOLE INHIBITION OF 24-HYDROXYLASE ACTIVITY ENHANCES FUNCTIONAL RESPONSE TO l,2S(OH)₂D₃ IN CACO-2 CELLS 341
117. QUANTITATIVE ANALYSIS OF CALCIUM ABSORPTION IN RATS SUBMITTED TO DIETS DIFFERING BY THE CONCENTRATION AND CHEMICAL FORM OF CALCIUM 343
118. DUODENAL TRANSPORT OF CALCIUM IN THE MARMOSET 345
119. INHIBITION OF LYSOSOMAL FUNCTIONS: EFFECT ON VITAMIN DDEPENDENT ABSORPTION OF ALUMINUM 347
120. Non-genomic Actions of Vitamin D Steroids
121. RECEPTOR MEDIATED RAPID ACTIONS OF STEROID HORMONES: cGMP ACCUMULATION AND REORGANIZATION OF RECEPTORS 351
122. VESICULAR CALCIUM TRANSPORT ACROSS THE INTESTINE AND ITS INITIATION BY 1,25-DIHYDROXYVITAMIN D3 DURING TRANSCALTACHIA 360
123. NON-GENE-MEDIATED EFFECTS OF CALCITRIOL: A POSSIBLE INVOLVEMENT OF MEMBRANE RECEPTOR 368
124. EFFECT OF DIETARY CALCIUM AND PHOSPHORUS DEFICIENCIES ON THE FLUIDITY OF PLASMA MEMBRANES OF CHICK DUODENAL ENTEROCYTES 376
125. DIRECT EFFECTS OF 1,25-(OH)₂D₃ AND 24,25-(OH)₂D₃ ON CHONDROCYTE MEMBRANE FLUIDITY 378
126. BRUSH BORDER MEMBRANE PROTEINS WITH INCREASED NUMBER OF HS" GROUPS AFTER TREATMENT WITH l,25(OH)₂D₃ AND OTHER HORMONES 380
127. NON GENOMIC EFFECTS OF CALCITRIOL ON PHOSPHOLIPID METABOLISM IN PORCINE PARATHYROID CELLS 382
128. INVOLVEMENT OF PROTEIN KINASE C IN 1,25(DH)₂-VITAMIN D₃ DEPENDENT MUSCLE Ca²⁺ UPTAKE 384
129. A STIMULATORY EFFECT OF I,25(OH)2D3 AND I (OH)D3 IN BLOOD SERUM ON THE Ca²⁺ UPTAKE BY ENTEROCYTE BRUSH BORDER 386
130. Cell Differentiation and Vitamin D
131. VIT D-INDUCED HL60 DIFFERENTIATION; PLASMALEMMA Ca-FUMP mRNA AND PKC ACTIVITY 391
132. RESPONSE TO l,25(OH)2 VITAMIN D₃ OF OSTEOBLASTIC CELLS ISOLATED FROM OVARIECTOMIZED RATS 393
133. PTH RELATED PEPTIDE (PTHrp) IS A POTENT DIFFERENTIATION FACTOR WHICH INDUCES THE MATURATION OF PERIPHERAL BLOOD MONOCYTES TO FORM MULTINUCLEATED GIANT CELLS IN CULTURE 395
134. VITAMIN D3 AND 1,25(OH)₂ VITAMIN D₃ INDUCED EXPRESSION OF CALBINDIN AND CALBINDIN mRNA IN INDIVIDUAL VILLUS-ATTACHED CHICKEN ENTEROCYTES 397
135. EFFECT OF 1,25-(OH)₂D₃ ON THE PROLIFERATION / MORPHOLOGICAL DIFFERENTIATION AND EPIDERMAL GROWTH FACTOR RECEPTORS (EGF-R) IN THE BREAST TUMOR BT-20 CELL LINE 399
136. CALCITRIOL ATTENUATES IODIDE UPTAKE IN A RAT THYROID CELL LINE 401
137. CALCITRIOL ATTENUATES cAMP PRODUCTION IN A RAT THYROID CELL LINE 403
138. 1,25-DIHYDROXYCHOLECALCIFEROL ENHANCES LATE FUSION AND DECREASES HLA-DR EXPRESSION IN CULTURED HUMAN CORD BLOOD, BUT NOT IN ADULT MONOCYTES 405
139. KINETICS OF IN VITRO MINERALIZATION BY AN OSTEOGENIC CLONAL CELL LINE DERIVED FROM MOUSE TERATOCARCINOMA 407
140. ROLE OF SECOND MESSENGERS IN THE STIMULATION OF MUSCLE CELL PROLIFERATION BY 1,25-DIHYDROXY-VITAMIN D₃ 409
141. DIFFERENTIATION-RELATED EFFECTS OF 1,25-DIHYDROXYCHOLECALCIFEROL ON INTESTINAL EPITHELIAL CELLS: AUXILIARY ROLE OF TRIIODOTHYRONINE 411
142. REGULATION OF PROLIFERATION AND ALKALINE PHOSPHATASE ACTIVITY IN THE HUMAN COLON ADENOCARCINOMA-DERIVED CELL LINE CACO-2 BY CALCIUM AND VITAMIN D 413
143. Skin and Vitamin D
144. EFFICACY AND TOLERABILITY OF CALCIPOTRIOL IN PSORIASIS 417
145. EPIDERMAL CYTOKERATIN AND IMMUNOCYTE RESPONSES DURING TREATMENT OF PSORIASIS WITH CALCIPOTRIOL 424
146. 1,25(0H),D3 ENHANCES ANTI-PROLIFERATIVE EFFECT AND TRANSCRIPTION OF TGF-β ON HUMAN KERATINOCYTES IN CULTURE 426
147. CYCLOSPORIN A DOES NOT INCREASE SERUM la,25(OH>₂D₃ IN PSORIASIS PATIENTS AND INHIBITS EXTRA-RENAL 1 a-HYDROXYLASE ACTIVITY 428
148. TREATMENT OF CHRONIC PLAQUE PSORIASIS BY la,25-DIHYDROXYVITAHIN D₃ OINTMENT 430
149. RICKETS IN RIYADH, A PUBLIC HEALTH PROBLEM 432
150. PHOTOPRODUCTION OF VITAMIN D3 IN CENTRAL AND SOUTHERN AREAS OF ARGENTINA 433
151. IOC,25-DIHYDROXYVITAMIN D₃: A NOVEL AGENT FOR WOUND HEALING 435
152. CHANGES IN EPIDERMAL KERATIN LEVELS DURING TREATMENT OF PSORIASIS WITH CALCIPOTRIOL (MC903) 437
153. IDENTIFICATION AND CHARACTERIZATION OF 1,25(OH)2D, TARGET CELLS AMONG SUBPOPULATIONS OF THE BASAL LAYER IN EPIDERMIS 439
154. PHARMACOLOGICAL ACTION OF l,24R(OH)₂D₃ IN EPIDERMAL CELL 441
155. EFFECT OF CALCIPOTRIOL ON PSORIASIS: A DOUBLE BLIND PLACEBO CONTROLLED STUDY OF CALCIPOTROL ( MC903) AND CALCIUM METABOLISM 443
156. MODULATION OF 1,25-DIHYDROXYVITAMIN D3 RECEPTOR (VDR) EXPRESSION IN HACAT KERATINOCYTES 445
157. Cancer and Vitamin D
158. A NOVEL VITAMIN D3 ANALOGUE, 22-0XA-1 a, 25-DIHYDROXYVITAMIN D3, INHIBITS THE GROWTH OF HUMAN BREAST CANCER WITHOUT CAUSING HYPERCALCEMIA 449
159. PERTURBATION OF VITAMIN D METABOLISM IN LYMPHOMA 451
160. 1,25(OH)2D3 INHIBITS CRYPT CELL PROLIFERATION INHUMAN COLORECTAL MUCOSA 453
161. EFFECT OF 1,25-DIHYDROXYVITAMIN D3 ( l , 2 5 - ( O H ) 2D3 ON 7.12 DIMETHYLBENZANTHRACENE (DMBA) INDUCED MAMMARY TUMORS IN SPRAGUEDAWLEY FEMALE RATS 455
162. ELEVATION IN CIRCULATING 1,25-DIHYDROXIVITAMIN D IN A PATIENT WITH T-CELL LYMPHOMA ASSOCIATED HYPERCALCEMIA 457
163. ANALYSIS OF 1,25 DIHYDROXYVITAMIN D₃ mRNA IN HUMAN BREAST CANCER TISSUES BY POLYMERASE CHAIN REACTION AND IN RAT MAMMARY TUMOURS BY NORTHERN BLOTS 459
164. TOPICAL VITAMIN D ANALOGUES IN ADVANCED BREAST CANCER 461
165. INTERACTION OF l,25(OH)2D3 AND TRANSFORMING GROWTH FACTOR IN HUMAN OSTEOSARCOMA CELLS 463
166. NOVEL VITAMIN D ANALOGUES SUPPRESS TUMOUR GROWTH IN VIVO 465
167. Immunology and Vitamin D
168. THE IMMUNOMODULATING PROPERTIES OF VITAMIN D: RECEPTORS AND MECHANISMS OF BIOLOGIC ACTION 469
169. NEW VITAMIN D₃ ANALOGUES WITH POTENT EFFECTS ON CELL GROWTH REGULATION AND IMMUNE RESPONSES: STRUCTURE-ACTIVITY STUDIES 478
170. REGULATION OF INTERLEUKIN-6 AND INTERLEUKIN-1β BY l,25(OH)₂D₃ IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) AND MONOCYTES: RELATIONSHIP TO LYMPHOCYTE PROLIFERATION AND POTENT MODULATION OF THE HORMONAL EFFECTS BY PHORBOL ESTERS 486
171. 1,25-DIHYDROXYVITAMIN D₃ ENHANCES THE EXOCYTOSIS OF CYTOTOXIC GRANULES FROM LYMPHOKINE-ACTIVATED KILLER CELLS 488
172. REGULATION OF INTERLEUKIN-ip GENE EXPRESSION BY 1,25- DIHYDROXYVITAMIN-D3 490
173. INHIBITION BY 1,25-DIHYDROXYVITAMIN D₃ OF INTERLEUKIN-2- STIMULATED HUMAN NATURAL KILLER CELLS 492
174. EFFECTS OF 22-0XA-1a,25-DIHYDROXYVITAMIN D₃ ON PRODUCTION OF TUMOR NECROSIS FACTOR-a AND INTERLEUKIN-13 BY HUMAN PERIPHERAL MONONUCLEAR CELLS 494
175. IN VITRO EFFECT OF 1,25-DIHYDROXYVITAMIN D3 (l,25-OH2D3) ON NK CELL CYTOTOXYCITY 496
176. ROLE OF 1,25-DIHYDROXYVITAMIN D₃ (l,25(OH)₂D₃) AS MODULATOR OF IMMUNOINFLAMMATORY REACTIONS 498
177. LOCAL INFLAMMATION INCREASES THE INHIBITORY EFFECT OF 1,25- DIHYDROXYVITAMIN D3 ON LYMPHOCYTE ACTIVATION 500
178. INTERACTION BETWEEN 1,25-DIHYDROXYVITAMIN D3 AND 17B-ESTRADI0L IN THE MODULATION OF LYMPHOCYTE ACTIVATION IN POSTMENOPAUSAL WOMEN 502
179. LOCAL RELEASE OF 1,25-VITAMIN D3 BY PROXIMAL TUBULAR CELLS DOWN-REGULATE ALLOGENIC LYMPHOCYTE RESPONSES 504
180. A COMPARISON OF THE EFFECTS OF 1,25 DIHYDROXYVITAMIN D3 (l,25(OH)2D3) AND THE SEX HORMONES ON IMMUNE FUNCTION 506
181. 1.25(OH)₂D₃ INCREASES NATURAL KILLER (NK)CELL ACTIVITY 512
182. ENHANCEMENT OF SKIN ALLOGRAFT SURVIVAL IN MICE BY ASSOCIATION OF 1-alpha HYDROXYVITAMIN D₃ TO INFRATHERAPEUTIC DOSES OF CYCLOSPORIN A 514
183. KH 1060 - A NEW VITAMIN D ANALOGUE WITH POTENT EFFECTS ON CELL-MEDIATED IMMUNE RESPONSES IN VITRO 516
184. IMMUNOSUPPRESSIVE EFFECTS OF 1,25-DIH YDROXYVITAMIN D3 ANALOGUE (CALCIPOTRIOL) ON EPIDERMAL CELLS 518
185. 1,25(OH)₂D₃ RECEPTOR EXPRESSION AND l,25(OH)2D3 ACTION ON MESANGIAL CELL PROLIFERATION 520
186. 1,25-DIHYDROXYVITAMIN D3 ENHANCES THE GENERATION OF NON-SPECIFIC SUPPRESSOR CELLS WHILE INHIBITING THE INDUCTION OF CYTOTOXIC CELLS IN A HUMAN MLR 522
187. Bone and Vitamin D
188. THE ROLE OF VITAMIN D IN BONE RESORPTION 527
189. 24R, 25(OH)₂D₃ INHIBITS FUSION OF COMMITTED PRECURSORS FOR HUMAN OSTEOCLAST-LIKE CELLS 535
190. 1,25R,26 (OH)3-22 ENE D3, A NOVEL THERAPEUTIC AGENT FOR REVERSAL OF BONE LOSS ASSOCIATED WITH CESSATION OF OVARIAN FUNCTION 537
191. REMINERALIZATION OF INCISOR DENTIN AND PLASMA CALCIUM IN RATS GIVEN VITAMIN D-DEFICIENT DIETS 539
192. INFLUENCE OF THYROID HORMONE ON OSTEOCALCIN SYNTHESIS BY HUMAN OSTEOBLAST-LIKE CELLS 541
193. l,25(OH)2D3 INDUCES HETEROGENEOUS DIFFERENTIATION OF HUMAN OSTEOBLAST CULTURES: EFFECT ON ALKALINE PHOSPHATASE AND OSTEOCALCIN EXPRESSION 543
194. EFFECT OF PARATHYROID HORMONE, 1,25 DIHYDROXYVITAMIN D3, TRANSFORMING GROWTH FACTOR BETA AND INSULIN-LIKE GROWTH FACTOR 1 ON PHOSPHATE HANDLING IN TWO OSTEOBLAST-LIKE CELL LINES 545
195. EFFECT OF 1,25(0H)zD3, 24,25(0H)₂D₃ AND 25(0H)D3 ON BONE ALKALINE PHOSPHATASE, A MARKER OF OSTEOBLAST FUNCTION 547
196. STRUCTURAL MODIFICATION INCREASES THE POTENCY OF 25(OH)D3 ON HUMAN BONE-DERIVED OSTEOBLAST-LIKE CELLS 549
197. INTERACTIONS BETWEEN SEX STEROIDS AND VITAMIN D METABOLITES IN THE REGULATION OF CREATINE KINASE ACTIVITY OF BONE 551
198. MODULATION OF 1,25(OH) ₂D₃ INDUCED OSTEOCALCIN SYNTHESIS IN HUMAN OSTEOSARCOMA CELLS BY TGF-β AND IGF-I 553
199. 25-OH-VITAMIN D AND CELIAC DISEASE ACTIVITY 555
200. BONE MINERAL DENSITY IN TREATED OSTEOMALACIA 557
201. EVIDENCE OF A ROLE FOR VITAMIN D AND TRANSFORMING GROWTH FACTOR P IN OSTEOINDUCTION 559
202. TOTAL BODY ABSORPTIOMETRY IN THE DIAGNOSIS AND FOLLOW-UP OF OSTEOMALACIAS. EFFECTS OF l,25(OH)2D3 TREATMENT 561
203. VITAMIN D RECEPTOR EXPRESSION AND 1.25-DIHYDROXYVITAMIN D₃ RESPONSIVENESS IN OSTEOBLAST-LIKE CELLS IN VITRO 563
204. 23(S),25(R)-1,25-DIHYDROXYVITAMIN D3-26,23-LACTONE IN BONE FORMATION 565
205. Calbindins: Structure-Function and Regulation
206. CALBINDIN-D9K (CaBP9K) GENE TISSUE-SPECIFIC REGULATION BY 1,25(OH)₂D₃ AND 17-β ESTRADIOL 575
207. VITAMIN D-DEPENDENT CALCIUM BINDING PROTEIN, CALBINDIN-D: REGULATION AND FUNCTIONAL CONSIDERATIONS 584
208. TISSUE-SPECIFIC SETS OF DNAsel HYPERSENSITIVE SITES ARE ASSOCIATED WITH EXPRESSION OF THE CALBINDIN D-9K (CaBP9k) GENE 591
209. CALBINDIN-D 9K (CaBP9K) GENE REGULATION IN THE RAT UTERUS DURING THE ESTROUS CYCLE 593
210. LOCALIZATION OF CALBINDIN-D28K AND PARVALBUMIN IN EXCITATORY AND INHIBITORY NEURONS OF THE PULVINAR-LATERALIS POSTERIOR COMPLEX 595
211. DIFFERENTIAL REGULATION BY 1,25-DIHYDROXYVITAMIN D3 OF CALBINDIN-D_9k AND CALBINDIN-D_28k GENE EXPRESSION IN HOUSE KIDNEY 597
212. IMMUNOCYTODETECTION OF CALBINDIN-D9K AND CALBINDIN-D_28K IN SKELETAL MUSCLES OF VITAMIN D-REPLETED AND VITAMIN-D-DEPRIVED RATS 599
213. IMMUNOREACTIVE l,25(OH)₂D₃, RECEPTORS AND CALBINDINS D-9k AND D-28k IN THE RAT EPIPHYSEAL CARTILAGE 601
214. EXPRESSION OF CALBEMDIN D-28K BY RAT NEURONS AND ASTROCYTES IN CULTURE 603
215. TRANSGENIC MICE AS POSSIBLE TOOLS FOR ELUCIDATING THE FUNCTION OF BRAIN CALBINDIN-D_28K 605
216. AUXILIARY VITAMIN D-INDEPENDENT FACTORS REGULATE CONSTITUTIVE NEURONAL CALBINDIN-D28K GENE EXPRESSION 607
217. SEARCH FOR A FUNCTION OF CALBINDINS 609
218. INTESTINAL CALBINDIN-D_9K AND CALCIUM ABSORPTION IN THE PIG 611
219. EFFECTS OF GH TREATMENT ON CIRCULATING LEVELS OF CALCITRIOL AND BONE CHARACTERISTICS IN YOUNG PIGS 613
220. CALBIND1N-D28K-LIKE IMMUNOREACTIVITY IN SPINAL AND DORSAL VAGAL COMPLEX PROJECTIONS IN RAT 615
221. SUBCELLULAR CO-LOCALIZATION OF CALBINDINS IN RAT AMELOBLASTS 617
222. NORTHERN AND PCR ANALYSIS OF CaBP-D28 EXPRESSION 619
223. VITAMIN D-CONTROLLED CO-EXPRESSION OF CALBINDIN GENES IN TEETH 621
224. IMMUNOHISTOCHEMICAL DEMONSTRATION OF CALBINDIN-D 28k IN GRANULE CELLS AND SYNAPTIC TERMINALS OF THE ELECTROSENSORY LOBE OF MORMYRID ELECTRIC FISH 623
225. HUMAN CALBINDIN-D9K: THE COMPLETE DERIVED AMINOACID SEQUENCE 625
226. EFFECTS OF VITAMIN D₃ DEFICIENCY AND REPLETION WITH 1,25 DIHYDROXYVITAMIN D₃ ON CALBINDIN D28K IN THE RAT ENDOCRINE PANCREAS 627
227. 1.25(OH)₂ D ALTERS THE EXPRESSION OF CALBINDIN-D_9k IN THE FEMALE MOUSE REPRODUCTIVE TRACT 629
228. IN-VITRO DEVELOPMENT OF RAT CEREBELLAR NEURONS OF EARLY EMBRYONIC ORIGIN. AN ANATOMICAL AND ELECTROPHYSIOLOGICAL STUDY 631
229. Assays for Vitamin D Steroids
230. ASSAYS FOR VITAMIN D AND ITS METABOLITES: DO WE NEED TO IMPROVE ACCURACY AND PRECISION? 635
231. NEW HIGH SENSITIVE, SPECIFIC, AND ACCURATE METHOD FOR ASSAYING VITAMIN D METABOLITES (THE FIRST PRACTICAL FLUOROMETRIC ASSAY METHOD FOR VITAMIN D) 644
232. 1,25(0H)₂ VITAMIN D RADIORECEPTOR ASSAY USING BOVINE MAMMARY GLAND RECEPTOR AND NON-HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC PURIFICATION 646
233. THERMOSPRAY MASS SPECTROMETRY OF 1,25-DIHYDROXYVITAMIN D₃ METABOLITES 648
234. USE OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY FOR THE MEASUREMENT OF DIHYDROTACHYSTEROL₂ AND TWO OF ITS METABOLITES IN HUMAN PLASMA 650
235. QUANTIFICATION AND KINETICS OF 25-HYDROXYVITAMIN D₃ USING STABLE ISOTOPE DILUTION AND THERMOSPRAY LIQUID CHROMATOGRAPHY/MASS SEPCTROMETRY (LC/MS) 652
236. A NOVEL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY FOR 1o HIDROXTVITAMIN D AND 1,25-DIHIDROHVITAMIN D IN HUMAN PLASMA USING A HIGH-SENSITIVE COULOMETRIC ELECTROCHEMICAL DETECTOR 654
237. MEASUREMENT OF CALCITRIOL LEVELS IN RATS 656
238. SIMULTANEOUS DETERMINATION OF 1,25-DIHYDROXYVITAMIN D₂ AND D₃ IN PLASMA USING CALF THYMUS RECEPTOR 658
239. ORAL CALCIDIOL INCREASES SERUM CALCITRIOL: ASSAY ARTIFACTS WITH LOW PRESSURE PURIFICATION 660
240. MICROMETHOD FOR THE DETERMINATION OF 25-HYDROXYVITAMIN D 662
241. DEVELOPMENT OF ENZYME IMMUNOASSAY FOR PLASMA 25-HYDROXYVITAMIN D₃: PRODUCTION AND APPLICATION OF [C-3]- and [C-11a]-ANTISERA 664
242. Other Basic Science Topics
243. NONCALCEMIC VITAMIN D ANALOGS AND MECHANISMS FOR THEIR SELECTIVITY 669
244. SITE-SPECIFIC CLEAVAGES OF PHOTOAFFINITY LABELLED HUMAN VITAMIN D-BINDING PROTEIN: IDENTIFICATION OF A 11.5 KDA PEPTIDE CONTAINING THE PUTATIVE 25-HYDROXYVITAMIN D₃ -BINDING DOMAIN 671
245. PHYLOGENY OF RECEPTORS FOR 1,25-DIHYDROXYVITAMIN D3 (1,25-D₃, soltriol) IN VERTEBRATES AND INVERTEBRATES 673
246. INTERACTIONS OF CALCITRIOL AND SEX STEROIDS IN RAT MYOMETRIAL CELLS 675
247. NONCLASSICAL HORMONAL ACTIONS OF CALCITRIOL (ROCALTROL ROCHE) 677
248. AN EVOLUTIONARY ASPECT IN VERTEBRATES FROM THE VIEWPOINT OF VITAMIN D METABOLISM 679
249. VITAMIN D3 IN CARTILAGINOUS FISH: IDENTIFICATION, QUANTIFICATION AND METABOLISM OF VITAMIN D₃ 681
250. THE CONFORMATIONAL CONTROL IN PROVITAMIN D PHOTOCHEMISTRY 683
251. VITAMIN D NUCLEAR BINDING TO NEURONS OF THE ALZHEIMER PATHOCLITIC REGION OF NUCLEUS BASALIS OF MEYNERT, CENTRAL AMYGDALOID GROUP AND NUCLEUS OF DIAGONAL BAND OF BROCA 685
252. VITAMIN D-SOLTRIOL: THE STEROID HORMONE OF SEASONAL REGULATION OF GROWTH AND REPRODUCTION, STRESS AND IMMUNE RESPONSE, NEURAL, ENDOCRINE-AUTONOMIC, NEUROMUSCULAR AND CARDIOVASCULAR FUNCTIONS 687
253. CLONING AND SEQUENCING A VITAMIN D INDUCIBLE PROTEIN FROM THE INTESTINE WITH AN EXTENSIVE LEUCINE ZIPPER MOTIF AND HOMOLOGY TO TROPOMYOSIN\MYOSIN 689
254. REGULATION OF CALMODULIN BINDING TO THE ATP EXTRACTABLE 110 kD PROTEIN FROM CHICKEN DUODENAL BRUSH BORDER 691
255. EFFECT OF 1,25 DIHYDROXYCHOLECALCIFEROL ON EGG PRODUCTION AND EGG SHELL QUALITY IN LAYING HENS 693
256. ACUTE EFFECT OF VITAMIN C ON VITAMIN D METABOLITES, PARATHORMONE AND Ca-Ph METABOLISM 695
257. EXPRESSION OF THE RAT VITAMIN D-BINDING PROTEIN GENE 697
258. Nutrition and Vitamin D
259. IS THERE A RECOMMENDED DIETARY ALLOWANCE FOR VITAMIN D ? 701
260. THE SEMANTICS OF VITAMIN D-DEFICIENCY 708
261. VITAMIN D DEFICIENCY RICKETS IN ITALY 710
262. RELATIONSHIP BETWEEN VITAMIN D SYSTEM AND ANTIEPILEPTIC DRUGS 712
263. THE FREQUENCY OF INSUFFICIENCY AND LOW LEVELS OF 25-HYDROXYVITAMIN D IN SAUDI POPULATION 714
264. MINERAL METABOLISM OP CHILDREN IN A VITAMIN D DEFICIENT AREA OF ARGENTINA 716
265. MAGNESIUM DEPLETION INDUCES HYPERCALCEMIA IN D3 REPLETE AND UP REGULATION OF THE INTESTINAL l,25(OH)2D3 RECEPTOR IN DDEPLETED RATS 718
266. CHOLECALCIFEROL SUPPLEMENTATION IMPROVES THE EFFICIENCY OF HINDGUT MICROBIAL FERMENTATION IN THE NAKED MOLE RAT, HETEROCEPHALUS GLABER 720
267. VITAMIN D INDEPENDENT MINERAL METABOLISM IN A SUBTERRANEAN MAMMAL, THE NAKED MOLE-RAT HETEROCEPHALUS GLABER 722
268. INFLUENCE OF CASEIN-DERIVED PHOSPHOPEPTIDES (CPP) ON THE BIOAVAILABILITY OF CALCIUM (CA) IN VITAMIN D-DEFICIENT (D-) MINIATURE PIGS 724
269. ESTROGEN STIMULATES INTESTINAL CALCIUM ABSORPTION IN AGED RATS 726
270. INTERACTION OP THE VITAMIN D HORMONE WITH OTHER VITAMINS 728
271. ANTIRACHITIC ACTIVITY OF TOPICAL I,25(OH)2D3 IN CHICKS 731
272. ANTIRACHITIC ACTIVITY OF VITAMIN D ETHERS AND ITS DEPENDENCE ON THE WAY OF ADMINISTRATION 733
273. DYNAMICS OP CALCIUM AND PHOSPHORUS CONTENT IN NAILS OF RACHITIC CHILDREN 735
274. Neonatology, Pregnancy, and Vitamin D
275. VITAMIN D IN INFANCY: 25-HYDROXYVITAMIN D, AN IMPORTANT BJOACTIVE PRINCIPLE IN VIVO IN INFANCY? 739
276. PERINATAL VITAMIN D METABOLISM 745
277. EFFICIENCY AND COMPLICATIONS OF CALCIUM AND PHOSPHORUS SUPPLEMENTATION IN VERY LOW BIRTH WEIGHT INFANTS 749
278. INFLUENCE OF VITAMIN D ON THE FETAL DEVELOPMENT OF THE RAT 751
279. EARLY WEANING INCREASES SERUM 1,25-DIHYDROXYVITAMIN D₃ LEVELS 753
280. Gerontology and Vitamin D
281. VITAMIN D NUTRITION IN THE ELDERLY; PROBLEMS AND RECOMMENDATIONS 757
282. COMPARATIVE EFFECTS OF EQUAL DOSES OF VITAMIN D2 AND VITAMIN D3 FOR THE CORRECTION OF VITAMIN D DEFICIENCY IN THE ELDERLY 765
283. 1,2 5 (OH )₂ D SERUM LEVEL AS A DETERMINANT OF 1,25(OH)2 D RESPONSE TO VITAMIN D THERAPY IN VITAMIN D-DEFICIENT ELDERLY SUBJECTS 767
284. AGE-RELATED PHARMACOKINETICS OF INTESTINAL CALCIUM ABSORPTION 771
285. 1.25 DIHYDROXYVITAMIN D IN ADULTS AND IN ELDERLY PATIENTS WITH OR WITHOUT HIP FRACTURES : INFLUENCE OF AGING 773
286. THE RELATIONSHIP BETWEEN VITAMIN D, CALCIUM ABSORPTION AND BONE MASS IN ELDERLY WOMEN 775
287. Osteoporosis and Vitamin D
288. EVALUATION OF CALCITRIOL IN THE TREATMENT OF POSTMENOPAUSAL OSTEOPOROSIS 779
289. CALCITRICIL TREATMENT OF OSTEOPOROSIS 787
290. ROLE OF CALCITRIOL IN THE PATHOPHYSIOLOGY AND TREATMENT OF INVOLUTIONAL OSTEOPOROSIS 798
291. 1-ALPHA-HYDROXYLATED METABOLITES OF VITAMIN D: THEIR PATHOPHYSIOLOGICAL AND THERAPEUTICAL ROLE IN POST-MENOPAUSAL OSTEOPOROSIS 807
292. ROLE OF CALCITRIOL IN PREVENTION OF OSTEOPOROSIS 816
293. LONG TERM TREATMENT OF POSTMENOPAUSAL OSTEOPOROSIS WITH 1,25 DIHYDROXYVITAMIN D₃ 823
294. CIRCULATING LEVELS OF 1,25-DIHYDROXYVITAMIN D DO NOT REFLECT TARGET-ORGAN LEVELS IN FEMALES 832
295. PREVENTION OF HIP FRACTURES IN THE ELDERLY BY VITAMIN D SUPPLEMENTATION 834
296. EFFECTS OF HIGH-DOSE 26,27-HEXAFLUORO-1 a ,25- DIHYDROXYCHOLECALCIFEROL(F6-1,25(OH) 2D3) ADMINISTRATION ON CORTICAL BONE REMODELING IN OVARIECTOMIZED DOGS 836
297. OSTEOPOROSIS 838
298. 1,25(0H)2D3 RECEPTOR LEVELS IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH POSTMENOPAUSAL OSTEOPOROSIS 840
299. VITAMIN D DEFICIENCY AND OSTEOPOROSIS 842
300. SECONDARY OSTEOPOROSIS DUE TO IRRADIATION 844
301. EFFECTS OF 17B-ESTRADIOL AND MEDROXYPROGESTERONE ON THE SERUM CONCENTRATION OF 1,25-DIHYDROXYVITAMIN D IN PERI-MENOPAUSAL WOMEN 845
302. Renal Osteodystrophy and Vitamin D
303. CALCITRIOL IN EARLY AND PRE-DIALYSIS RENAL FAILURE: WHAT ARE THE RISKS AND BENEFITS? 849
304. VITAMIN D - THE ENDOCRINE SYSTEM AND SECONDARY HYPERPARATHYROIDISM 857
305. 24,25-DIHYDROXYVITAMIN D3 TREATMENT INHIBITS PARATHYROID HORMONE STIMULATED ADENYLATE CYCLASE ACTIVITY IN BONE BIOPSIES OF UREMIC PATIENTS 863
306. DO CHANGES IN PHOSPHATE BALANCE MODULATE l,25(OH)₂D₃ LEVELS IN HEALTHY CONTROLLS AND IN PATIENTS WITH EARLY RENAL FAILURE ? 865
307. EVIDENCE FOR DISTURBED REGULATION OF 1,25-DIHYDROXYVITAMIN D₃ IN EARLY CHRONIC RENAL FAILURE 867
308. COMPARISON OF INTRAVENOUS ALFACIDOL IN ASSOCIATION WITH Mg(OH)2 AS SOLE PHOSPHATE BINDER TO THE ASSOCIATION ORAL CaCO3+Mg(OH(2 IN THE PREVENTION OF HYPERPARATHYROIDISM IN DIALYSIS PATIENTS 869
309. REVERSAL OF SEVERE HYPERPARATHYROIDISM IN HEMODIALYZED PATIENTS BY INTRAVENOUS ALFACALCIDOL AND CaCO3 AS SOLE PHOSPHATE BINDER ASSOCIATED WITH A LOW DIALYSATE CALCIUM 871
310. PROGRESSION OF SECONDARY HYPERPARATHYROIDISM DESPITE ORAL CALCITRIOL THERAPY IN PEDIATRIC PATIENTS ON PERITONEAL DIALYSIS (CAPD/CCPD) 873
311. EFFICACY OF INTRAVENOUS (IV.) CALQTRIOL (C) IN SHIFTING THE IONIZED CALCIUM (Ca++)/PTH CURVE AND REDUCING THE SET POINT OF CALCIUM IN DIALYSIS PATIENTS WITH REFRACTORY SECONDARY HYPERPARATHYROIDISM (HPT) 875
312. EFFECTS OF 1,25(OH)₂D₃ ON COMPENSATORY RENAL GROWTH IN THE GROWING RAT 877
313. LOW DOSES OF ORAL CALCITRIOL RETARD PROGRESSION OF NATURALLY OCCURRING RENAL DISEASE OF THE DOG 879
314. ERYTHROPOIETIN DOSE REQUIREMENT IN PATIENTS TREATED WITH ORAL CALCITRIOL OR PARATHYROIDECTOMY 881
315. Other Clinical Topics
316. OSTEOCALCIN LEVELS AT ONSET OF TYPE I DIABETES IN CHILDREN AND ADOLESCENTS 885
317. BONE METABOLISM IN SCREENED CONGENITAL HYPOTHYROID INFANTS 887
318. PREVALENCE OF OSTEOPENIA IN GLUTEN ENTEROPATHY 889
319. VITAMIN D ₃ DEFICIENCY AND CARDIAC SLOW CALCIUM CHANNELS 891
320. COMPARISON AMONG COMMERCIAL KIT METHODS AND HPLCRADIORECEPTOR ASSAY FOR 1,25-DIHYDROXYVITAMIN D. 893
321. KETOCONAZOLE ADMINISTRATION DECREASES 1,25-DIHYDROXYVITAMIN D AND IONIZED CALCIUM CONCENTRATIONS IN TUBERCULOSIS-ASSOCIATED HYPERCALCEMIA 895
322. INFANTILE HYPERCALCEMIA WITH SUBCUTANEOUS FAT NECROSIS (SFN) IS DUE TO INCREASED 1,25-DIHYDROXYVITAMIN D PRODUCTION 897
323. LOW SERUM CALCITRIOL CONCENTRATIONS AND REDUCED BONE FORMATION IN PATIENTS WITH SEVERE BURNS 899
324. VITAMIN D AND CELL MEDIATED FUNCTION IN INMIGRANTS OF INDIAN SUBCONTINENT EIHNIC ORIGIN TO THE UK 901
325. ABNORMAL REGULATION OF PLASMA 1,25-DIHYDROXYVITAMIN D IN GYRO (Gy, X-LINKED HYPOPHOSPHATEMIC) MICE 903
326. VITAMIN D METABOLISM AMD BONE MINERALIZATION IN CHILDREN WITH JUVENILE RHEUMATOID ARTHRITIS (JRA) 905
327. URINARY CALCIUM AND SERUM l,25(OH)2D CORRELATE IN KIDNEY STONE PATIENTS 907
328. ATTAINED ADULT HEIGHT IN HYPOPHOSPHATEMIC VITAMIN D RESISTANT RICKETS 909
329. OSTEOCALCIN AND VITAMIN D METABOLITES IN 0- THALASSAEMIA BEFORE AND DURING ADOLESCENCE 911
330. CALCIUM BALANCE AND SERUM VITAMIN D METABOLITES AFTER ORAL SINGLE DOSE OF 25 (OH) D IN PATIENTS WITH TUBERCULOSIS 913
331. EFFECTS OF A HUMAN BED REST MODEL FOR SPACEFLIGHT ON SERUM 1,25- DIHYDROXYVITAMIN D 915
332. COS-1 CELLS TRANSFECTED WITH pSVL PLASMID CONTAINING KIDNEY MITOCHONDRIAL CYTOCHROME P-450_1A( cDNA EXPRESS 25-OH-D₃-HYDROXYLASE ACTIVITIES 917
333. PATHOGENESIS OF CANCER-ASSOCIATED HYPERCALCEMIA: CHARACTERIZATION OF POLYCLONAL ANTIBODIES TO PARATHYROID HORMONE-RELATED PROTEIN IN CHICKENS 919
334. 1 a HYDROXYCHOLECALCIFEROL IN TREATMENT OF JUVENILE 921
335. SERUM 25-HYDROXYVITAMIN D CONCENTRATION IN SUDDEN INFANT DEATH SYNDROME 922
336. INCREASED INCIDENCE OF TB IN U.K. IMMIGRANT ASIANS AT GREATEST RISK OF VITAMIN D DEFICIENCY 924
337. A NEW KINDRED WITH HEREDITARY HYPOPHOSPHATEMIC RICKETS WITH HYPERCALCIURIA (HHRH). IMPLICATIONS FOR CORRECT DIAGNOSIS AND TREATMENT 926
338. SERUM CONCENTRATIONS OF CALCIOTROPIC HORMONES AND BIOCHEMICAL INDICES OF BONE TURNOVER IN SYSTEMIC LUPUS ERYTHEMATOSUS 928
339. CYCLOSPORIN-A THERAPY IN RENAL TRANSPLANTED CHILDREN AND MINERAL METABOLISM 930
340. RECURRENT IDIOPATHIC CALCIUM UROLITHIASIS (RCU) - BLOOD LEVELS OF TWO VITAMIN D METABOLITES IN MALES 932
341. TREATMENT OF SECONDARY HYPERPARATHYROIDISM BY INTRAVENOUS CALCITRIOL 934
342. VITAMIN D:CUTANEOUS PRODUCTION AND THERAPEUTIC EFFICACY IN PSORIASIS 940
343. EFFECT OF 1,25 DIHYDROXIVITAMIN D AND 24,25 DIHYDROXIVITAMIN D ON OSTEOCALCIN SYNTHESIS IN CULTURED FETAL RAT CALVARIAS 949
344. DIAGNOSTICAL APPLICATION OF IMMUNOCYTOCHEMICAL STAINING ON CALBINI>IN-D(28K) IN HUMAN KIDNEY DISEASE 951
345. POST-TRANSCRIPTION AL REGULATION OF HUMAN T LYMPHOCYTE ACTIVATION: BY 1,25-DIHYDROXYVITAMIND3^*+# 953
346. AUTHOR INDEX 961
347. KEY WORD INDEX 971
348. CELL LINE INDEX 992
349. VITAMIN D WORKSHOP ZOO 993
350. SUMMARY OF THE DATES AND LOCATIONS OF THE EIGHT VITAMIN D WORKSHOPS 994
351. VITAMIN D WORKSHOP LOGO 995
352. Backmatter 997