Chapter 11 Cupriavidus necator – a broadly applicable aerobic hydrogen-oxidizing bacterium
-
Hannah Wohlers
Abstract
Cupriavidus necator (previously Ralstonia eutropha) is a highly flexible chemolithoautotrophic bacterium capable of oxidizing molecular hydrogen to serve as the sole electron source and assimilate inorganic carbon. Furthermore, this aerobic Knallgas microorganism can also rely on carbon sources to drive its metabolism in a heterotrophic fashion. This metabolic flexibility makes C. necator a potentially invaluable tool for a plethora of applications including the production of chemicals and bioplastic precursors as well as hydrogen-driven cofactor regeneration and biocatalysis. In this chapter, the complex metabolism of C. necator and key players of autotrophic growth (hydrogenases) are introduced and summarized. Furthermore, state-of-the-art cultivation techniques and methodologies for the biotechnological utilization of C. necator are highlighted.
Abstract
Cupriavidus necator (previously Ralstonia eutropha) is a highly flexible chemolithoautotrophic bacterium capable of oxidizing molecular hydrogen to serve as the sole electron source and assimilate inorganic carbon. Furthermore, this aerobic Knallgas microorganism can also rely on carbon sources to drive its metabolism in a heterotrophic fashion. This metabolic flexibility makes C. necator a potentially invaluable tool for a plethora of applications including the production of chemicals and bioplastic precursors as well as hydrogen-driven cofactor regeneration and biocatalysis. In this chapter, the complex metabolism of C. necator and key players of autotrophic growth (hydrogenases) are introduced and summarized. Furthermore, state-of-the-art cultivation techniques and methodologies for the biotechnological utilization of C. necator are highlighted.
Chapters in this book
- Frontmatter I
- Contents V
- List of authors VII
- Chapter 1 A short recapitulation of the autotrophic metabolism 1
- Chapter 2 Metabolic engineering of microbes 19
- Chapter 3 Protein engineering 47
- Chapter 4 Gas fermentation 85
- Chapter 5 Introduction to autotrophic cultivation of microalgae in photobioreactors 113
- Chapter 6 Synthetic biology of cyanobacteria 131
- Chapter 7 Algal biotechnology 173
- Chapter 8 Biocatalytic applications of autotrophic organisms 207
- Chapter 9 Photocatalysis to promote cell-free biocatalytic reactions 247
- Chapter 10 Electroautotrophs: feeding microbes with current for CO2 fixation 277
- Chapter 11 Cupriavidus necator – a broadly applicable aerobic hydrogen-oxidizing bacterium 297
- Chapter 12 Poly(3-hydroxybutyrate) as renewable resource 319
- Chapter 13 Applications of mixed microbial cultures in industrial biotechnology 353
- Chapter 14 Economic framework of autotrophic processes 385
- Index 397
Chapters in this book
- Frontmatter I
- Contents V
- List of authors VII
- Chapter 1 A short recapitulation of the autotrophic metabolism 1
- Chapter 2 Metabolic engineering of microbes 19
- Chapter 3 Protein engineering 47
- Chapter 4 Gas fermentation 85
- Chapter 5 Introduction to autotrophic cultivation of microalgae in photobioreactors 113
- Chapter 6 Synthetic biology of cyanobacteria 131
- Chapter 7 Algal biotechnology 173
- Chapter 8 Biocatalytic applications of autotrophic organisms 207
- Chapter 9 Photocatalysis to promote cell-free biocatalytic reactions 247
- Chapter 10 Electroautotrophs: feeding microbes with current for CO2 fixation 277
- Chapter 11 Cupriavidus necator – a broadly applicable aerobic hydrogen-oxidizing bacterium 297
- Chapter 12 Poly(3-hydroxybutyrate) as renewable resource 319
- Chapter 13 Applications of mixed microbial cultures in industrial biotechnology 353
- Chapter 14 Economic framework of autotrophic processes 385
- Index 397
