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Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma

  • Karin Gewert , Peter C. Gregory , Gunilla Olivecrona , Charlotte Erlanson-Albertsson and Stefan G. Pierzynowski
Published/Copyright: October 19, 2005
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 43 Issue 11

Abstract

The aim of this study was to investigate the specificity of the 3H-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the 3H-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the 3H-triolein assay designed for pancreatic lipase, but the activity did not exceed 1% of the activity of this enzyme measured in an assay specific for lipoprotein lipase (samples containing physiological levels of lipoprotein lipase did not show any activity in the assay). Hepatic lipase was not measurable under the conditions of the 3H-triolein assay. In conclusion, the 3H-triolein assay showed pronounced specificity for pancreatic lipase compared with lipoprotein lipase or hepatic lipase.

Keywords: hepatic lipase; lipoprotein lipase; pancreatic lipase
Corresponding author: Dr. Stefan G. Pierzynowski, Department of Cell and Organism Biology, Animal Physiology, Lund University, Helgonav. 3b, SE-223 62 Lund, Sweden Phone: +46-46-2224381, Fax: +46-46-2224539,

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Received: 2005-3-7
Accepted: 2005-8-21
Published Online: 2005-10-19
Published in Print: 2005-11-1

©2005 by Walter de Gruyter Berlin New York

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  4. Failure of the PAXgene™ Blood RNA System to maintain mRNA stability in whole blood
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  7. Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma
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  14. Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
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https://doi.org/10.1515/CCLM.2005.209
Keywords for this article
hepatic lipase; lipoprotein lipase; pancreatic lipase

Articles in the same Issue

  1. Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region
  2. Isotretinoin therapy induces DNA oxidative damage
  3. Structural evaluation of plasma α2-macroglobulin in acute pancreatitis
  4. Failure of the PAXgene™ Blood RNA System to maintain mRNA stability in whole blood
  5. Reticulocyte hemoglobin measurement – comparison of two methods in the diagnosis of iron-restricted erythropoiesis
  6. Physical analysis of ejaculate to evaluate the secretory activity of the seminal vesicles and prostate
  7. Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma
  8. Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice
  9. Plasma levels of 7-hydroxymetabolites of dehydroepiandrosterone in healthy Central European aging men
  10. Prevalence of abnormal thyroid stimulating hormone and thyroid peroxidase antibody-positive results in a population of pregnant women in the Samara region of the Russian Federation
  11. Impact of standardized calibration on the inter-assay variation of 14 automated assays for the measurement of creatinine in human serum
  12. Efficacy of three ELISA measurements of anti-cyclic citrullinated peptide antibodies in the early diagnosis of rheumatoid arthritis
  13. Mean and variance quality control for multiple correlated levels of replicated control samples
  14. Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
  15. Analytical evaluation of the Dade Behring Dimension RxL automated N-Terminal proBNP (NT-proBNP) method and comparison with the Roche Elecsys 2010
  16. Unusually high alkaline phosphatase due to intestinal isoenzyme in a healthy adult
  17. Deficient α-galactosidase A activity in plasma but no Fabry disease – a pitfall in diagnosis
  18. Reportable interval of patient examination results and ISO 15189
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