Letter to the Editor: “Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase”
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Burcu Baba
und
Ayşegül Akbay
To the Editor,
Turkish Journal of Biochemistry
Dear Editor,
The article written by Qiu et al. on “Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase” is very interesting [1]. We are writing to address a well-recognized challenge in recombinant protein production: the insolubility of bacterially expressed eukaryotic proteins. This issue often arises due to the differences in post-translational modifications, chaperone systems, and cellular environments between prokaryotic and eukaryotic cells. Many eukaryotic proteins, when expressed in Escherichia coli, tend to aggregate into inclusion bodies, rendering them inactive and difficult to purify [2], 3].
To overcome this challenge, several strategies have been explored. One approach is the use of fusion tags such as maltose-binding protein (MBP) or small ubiquitin-like modifier (SUMO), which can enhance solubility and facilitate proper folding. Another widely employed strategy is co-expression with molecular chaperones or foldases that assist in protein folding. Additionally, optimizing expression conditions – such as reducing the growth temperature, using weaker promoters, or supplementing the culture medium with specific additives like osmolytes – can significantly improve solubility [2], 3].
In cases where solubility remains an issue, refolding inclusion body proteins using stepwise dialysis or chromatographic techniques may be necessary. Alternatively, shifting to eukaryotic expression systems, such as yeast, insect, or mammalian cells, can provide a more suitable environment for proper folding and functionality [3], 4].
Given the increasing reliance on bacterial systems for cost-effective protein production, further research into novel solubilization strategies is crucial. We encourage continued discussion and innovation in this field to facilitate the production of functional eukaryotic proteins for biochemical and biomedical research.
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Research ethics: Not applicable.
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Informed consent: Not applicable.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The authors declare to have no competing interests.
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Research funding: None declared.
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Data availability: Not applicable.
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Consent for publication: Not applicable.
References
1. Qiu, M, Dai, X, Hu, J, Zhang, J, Liu, Q, Luan, J, et al.. Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase. Turk J Biochem 2025;50:116–25. https://doi.org/10.1515/tjb-2024-0191.Suche in Google Scholar
2. Bhatwa, A, Wang, W, Hassan, YI, Abraham, N, Li, XZ, Zhou, T. Challenges associated with the formation of recombinant protein inclusion bodies in Escherichia coli and strategies to address them for industrial applications. Front Bioeng Biotechnol 2021;9:630551. https://doi.org/10.3389/fbioe.2021.630551.Suche in Google Scholar PubMed PubMed Central
3. Eskandari, A, Nezhad, NG, Leow, TC, Rahman, MBA, Oslan, SN. Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli. Arch Microbiol 2024;206:152. https://doi.org/10.1007/s00203-024-03871-2.Suche in Google Scholar PubMed
4. Nabiel, A, Yosua, Y, Sriwidodo, S, Maksum, IP. Overview of refolding methods on misfolded recombinant proteins from Escherichia coli inclusion bodies. J Appl Biol Biotechnol 2023;11:47–52. https://doi.org/10.7324/JABB.2023.112204.Suche in Google Scholar
© 2025 the author(s), published by De Gruyter, Berlin/Boston
This work is licensed under the Creative Commons Attribution 4.0 International License.
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- Letter to the Editor: “Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase”
Artikel in diesem Heft
- Frontmatter
- Review Article
- Unveiling the hidden clinical and economic impact of preanalytical errors
- Research Articles
- To explore the role of hsa_circ_0053004/hsa-miR-646/CBX2 in diabetic retinopathy based on bioinformatics analysis and experimental verification
- Study on the LINC00578/miR-495-3p/RNF8 axis regulating breast cancer progression
- Comparison of two different anti-mullerian hormone measurement methods and evaluation of anti-mullerian hormone in polycystic ovary syndrome
- The evaluation of the relationship between anti angiotensin type I antibodies in hypertensive patients undergoing kidney transplantation
- Evaluation of neopterin, oxidative stress, and immune system in silicosis
- Assessment of lipocalin-1, resistin, cathepsin-D, neurokinin A, agmatine, NGF, and BDNF serum levels in children with Autism Spectrum Disorder
- Regulatory nexus in inflammation, tissue repair and immune modulation in Crimean-Congo hemorrhagic fever: PTX3, FGF2 and TNFAIP6
- Pasteur effect in leukocyte energy metabolism of patients with mild, moderate, and severe COVID-19
- Thiol-disulfide homeostasis and ischemia-modified albumin in patients with sepsis
- Myotonic dystrophy type 1 and oxidative imbalance: evaluation of ischemia-modified albumin and oxidant stress
- Antioxidant and alpha-glucosidase inhibitory activities of flavonoids isolated from fermented leaves of Camellia chrysantha (Hu) Tuyama
- Examination of the apelin signaling pathway in acetaminophen-induced hepatotoxicity in rats
- Integrating network pharmacology, in silico molecular docking and experimental validation to explain the anticancer, apoptotic, and anti-metastatic effects of cosmosiin natural product against human lung carcinoma
- Validation of Protein A chromatography: orthogonal method with size exclusion chromatography validation for mAb titer analysis
- The evaluation of the efficiency of Atellica UAS800 in detecting pathogens (rod, cocci) causing urinary tract infection
- Case Report
- Exploring inherited vitamin B responsive disorders in the Moroccan population: cutting-edge diagnosis via GC-MS profiling
- Letter to the Editor
- Letter to the Editor: “Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase”
