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miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia
-
Frank Noack
,
Julika Ribbat-Idel
Veröffentlicht/Copyright:
10. Februar 2011
Abstract
Aims: Micro RNAs (miRNAs) are small, single-strand RNAs, playing an important role in post-transcriptional gene regulation. The placenta is considered to play a key role in pathogenesis of preeclampsia. The purpose of this study was to demonstrate deregulation of miRNAs in placentas with preeclampsia using formalin-fixed and paraffin-embedded (FFPE) tissues.
Methods: Expression levels of 162 miRNAs were measured in FFPE placental tissues (5 with severe preeclampsia, 5 from a control group) using a quantitative qPCR based technique.
Results: Six miRNAs were more than 2-fold over-expressed in severe preeclampsia: let-7b, miRNA-302*, miRNA-104, miRNA-128a, miRNA-182* and miRNA-133b. Gene ontology analyses were performed using the algorithms “TargetScanS”, “microRNA”, and “PicTar”.
Conclusions: Two of the up-regulated miRNAs (miRNA-182* and miRNA-133b) are putative regulators of the transcript variants 1 and 2 of the BCL2-like gene, which controls apoptosis. miRNA-182* is also a probable angiogenesis regulator via angiogenin and VEGF-B. Apoptosis and angiogenesis are major mechanisms presumed to be involved in the pathogenesis of preeclampsia. Moreover, usability of qPCR technique based miRNA profiling for FFPE tissues was proofed. Hence FFPE tissue is the most widely used material for retrospective clinical studies, this method has a great property for future investigations in placenta research.
Keywords: Expression profiling; formalin-fixed and para-ffin-embedded (FFPE); miRNA; placenta; preeclampsia
Received: 2010-6-22
Revised: 2010-10-22
Accepted: 2010-11-3
Published Online: 2011-02-10
Published Online: 2011-02-10
Published in Print: 2011-05-01
©2011 by Walter de Gruyter Berlin New York
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- Calendar
- Congress Calendar
Schlagwörter für diesen Artikel
Expression profiling;
formalin-fixed and para-ffin-embedded (FFPE);
miRNA;
placenta;
preeclampsia
Artikel in diesem Heft
- Publisher’s Note
- Publisher’s Note
- EDITORIAL
- Problems in prevention of preterm birth – regrettable contradictions
- REVIEW ARTICLES
- Listeriosis in human pregnancy: a systematic review
- Prevention of prematurity by single embryo transfer
- ORIGINAL ARTICLES - OBSTETRICS
- Effect of prior cesarean delivery on neonatal outcomes
- Amniotic lamellar body counts can predict the occurrence of respiratory distress syndrome as well as transient tachypnea of the newborn (TTN)
- Spectrum of cardiovascular findings during pregnancy and parturition at a tertiary referral center
- Risk groups and maternal-neonatal complications of preeclampsia – Current results from the national German Perinatal Quality Registry
- miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia
- Genetic polymorphisms of killer cell immunoglobulin-like receptor 3DL2 in preeclampsia
- Addressing concerns about cisplatin application during pregnancy
- Retinol-binding protein-4 is decreased in patients with preeclampsia in comparison with normal pregnant women
- Somatic classification of neonates based on birth weight, length, and head circumference: quantification of the effects of maternal BMI and smoking
- Prediction of successful labor induction by evaluation of maternal symptoms at an early stage of the misoprostol induction protocol
- ORIGINAL ARTICLES - FETUS
- Increased nuchal translucency is associated with large for gestational age neonates in singleton pregnancies
- ORIGINAL ARTICLES - NEWBORN
- Predicting mortality in infants with persistent pulmonary hypertension of the newborn with the Score for Neonatal Acute Physiology-Version II (SNAP-II) in Thai neonates
- Neonatal outcome of preterm discordant twins
- Effects of delayed umbilical cord clamping on peripheral blood hematopoietic stem cells in premature neonates
- In utero exposure to Ureaplasma spp. is associated with increased rate of bronchopulmonary dysplasia and intraventricular hemorrhage in preterm infants
- Does prenatal antibiotic therapy compromise the diagnosis of early-onset infection and management of the neonate?
- SNAPPE-II application in newborns with very low birth weight: evaluation of adverse outcomes in severe placental dysfunction
- SHORT COMMUNICATION
- Lamellar bodies: platelet channel particles as predictors of respiratory distress syndrome (RDS) and of transient tachypnea of the newborn
- Intrahepatic and adrenal hemorrhage as a rare cause of neonatal anemia
- Subcutaneous fat distribution in small for gestational age newborns
- Calendar
- Congress Calendar
