Comparison of five automated hematology analyzers in a university hospital setting: Abbott Cell-Dyn Sapphire, Beckman Coulter DxH 800, Siemens Advia 2120i, Sysmex XE-5000, and Sysmex XN-2000
-
Mathias Bruegel
,
Dorothea Nagel
Abstract
Background: Various types of automated hematology analyzers are used in clinical laboratories. Here, we performed a side-by-side comparison of five current top of the range routine hematology analyzers in the setting of a university hospital central laboratory.
Methods: Complete blood counts (CBC), differentials, reticulocyte and nucleated red blood cell (NRBC) counts of 349 patient samples, randomly taken out of routine diagnostics, were analyzed with Cell-Dyn Sapphire (Abbott), DxH 800 (Beckman Coulter), Advia 2120i (Siemens), XE-5000 and XN-2000 (Sysmex). Inter-instrument comparison of CBCs including reticulocyte and NRBC counts and investigation of flagging quality in relation to microscopy were performed with the complete set of samples. Inter-instrument comparison of five-part differential was performed using samples without atypical cells in blood smear (n=292). Automated five-part differentials and NRBCs were additionally compared with microscopy.
Results: The five analyzers showed a good concordance for basic blood count parameters. Correlations between instruments were less well for reticulocyte counts, NRBCs, and differentials. The poorest concordance for NRBCs with microscopy was observed for Advia 2120i (Kendall’s τb=0.37). The highest flagging sensitivity for blasts was observed for XN-2000 (97% compared to 65%–76% for other analyzers), whereas overall specificity was comparable between different instruments.
Conclusions: To the best of our knowledge, this is the most comprehensive side-by-side comparison of five current top of the range routine hematology analyzers. Variable analyzer quality and parameter specific limitations must be considered in defining laboratory algorithms in clinical practice.
Acknowledgments
We thank Abbott, Beckman Coulter, Siemens and Sysmex for their support of the study by providing their instruments for evaluation free of charge.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Financial support: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplemental Material
The online version of this article (DOI: 10.1515/cclm-2014-0495) offers supplementary material, available to authorized users.
©2015 by De Gruyter
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Artikel in diesem Heft
- Frontmatter
- Editorial
- Personalized medicine: moving from simple theory to daily practice
- Evaluating and using innovative technologies: a lesson from Theranos?
- Review
- Emerging biomarkers in the detection and prognosis of prostate cancer
- Mini Review
- Could molecular assessment of calcium metabolism be a useful tool to early screen patients at risk for pre-eclampsia complicated pregnancy? Proposal and rationale
- Opinion Papers
- Is laboratory medicine ready for the era of personalized medicine? A survey addressed to laboratory directors of hospitals/academic schools of medicine in Europe
- Theranos phenomenon: promises and fallacies
- Clinical laboratories: production industry or medical services?
- Genetics and Molecular Diagnostics
- Role of JAK2 V617F mutation and aberrant expression of microRNA-143 in myeloproliferative neoplasms
- Direct identification of Gram-positive bacteria and resistance determinants from blood cultures using a microarray-based nucleic acid assay: in-depth analysis of microarray data for undetermined results
- General Clinical Chemistry and Laboratory Medicine
- Comparison between bottom-up and top-down approaches in the estimation of measurement uncertainty
- Role of vitamin D and sFlt-1/PlGF ratio in the development of early- and late-onset preeclampsia
- Adenosine deaminase, dipeptidyl peptidase-IV activities and lipid peroxidation are increased in the saliva of obese young adult
- PON-1 and ferroxidase activities in older patients with mild cognitive impairment, late onset Alzheimer’s disease or vascular dementia
- Comparison of five automated hematology analyzers in a university hospital setting: Abbott Cell-Dyn Sapphire, Beckman Coulter DxH 800, Siemens Advia 2120i, Sysmex XE-5000, and Sysmex XN-2000
- Dacryocytes are a common morphologic feature of autoimmune and microangiopathic haemolytic anaemia
- Performance characteristics of a new automated method for measurement of anti-cyclic citrullinated peptide
- The clinical performance of a chemiluminescent immunoassay in detecting anti-cardiolipin and anti-β2 glycoprotein I antibodies. A comparison with a homemade ELISA method
- Reference Values and Biological Variations
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- Development of the first urinary reproductive hormone ranges referenced to independently determined ovulation day
- Cancer Diagnostics
- Urinary miR-183 and miR-205 do not surpass PCA3 in urine as predictive markers for prostate biopsy outcome despite their highly dysregulated expression in prostate cancer tissue
- Cardiovascular Diseases
- Elevated circulating levels of lipoprotein-associated phospholipase A2 in obese children
- Letters to the Editors
- Reporting of hemolysis index (HI) with laboratory results should be obligatory in newborns and infants
- Unmeasurably high chloride: a surrogate marker of thiocyanate poisoning identification
- Association between red cell distribution width and myocardial infarction in rheumatoid arthritis
- Plasmatic and urinary glycosaminoglycan profile in a patient affected by multiple sulfatase deficiency
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