Startseite Lebenswissenschaften The alleviating effects of salicylic acid application against aluminium toxicity in barley (Hordeum vulgare) roots
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

The alleviating effects of salicylic acid application against aluminium toxicity in barley (Hordeum vulgare) roots

  • Gizem Yalcin und Filiz Vardar EMAIL logo
Veröffentlicht/Copyright: 12. Januar 2017
Veröffentlichen auch Sie bei De Gruyter Brill
Biologia
Aus der Zeitschrift Biologia Band 71 Heft 12

Abstract

Aluminium (Al) toxicity is one of the major growth limiting factors that affects large agricultural areas resulting in reduced crop production in acid soils. The present study aims to investigate alleviating effects of salicylic acid (SA) on Al toxicity in barley (Hordeum vulgare L.) roots. The roots were exposed to 20 µM AlCl3 with or without SA (5 and 10 µM) for 72 h. In an alternative group, roots were pre-treated with 5 µM and 10 µM SA for 24 h, and then they were exposed to 20 µM AlCl3. To evaluate the ameliorating effects of SA on Al toxicity some cellular stress responses were investigated including root elongation, Al uptake, loss of plasma membrane integrity, mitotic abnormalities, superoxide dismutase activity, peroxidase activity, total protein content and DNA fragmentation. The obtained results suggested that both pre-treatment and co-treatment of 10 µM SA could alleviate Al-induced toxicity in barley roots in relation to inhibition of Al uptake and activation of the antioxidant enzyme system.

Acknowledgements

This work was supported by the Research Foundation of Marmara University (BAPKO) under Grant (no. FEN-CYLP-141014-0349).

References

Aytürk Ö. & Vardar F. 2014. Aluminum-induced caspase-like activities in some Gramineae species. Adv. Food Sci. 37: 71–75Suche in Google Scholar

Alvarez M.E. 2000. Salicylic acid in the machinery of hypersensitive cell death and disease resistance. Plant Mol. Biol.44: 429–442.10.1007/978-94-010-0934-8_14Suche in Google Scholar

Ananieva E.A., Alexieva V.S. & Popova L.P. 2002. Treatment with salicylic acid decreases the effects of paraquat on photosynthesis. J. Plant Physiol. 159: 685–693.10.1078/0176-1617-0706Suche in Google Scholar

Birecka H., Briber K.A. & Catalfamo J.L. 1973. Comparative studies on tobacco pit and sweet potato root isoperoxidases in relation to injury, indolacetic acid and ethylene effects. Plant Physiol.52: 43–49.10.1104/pp.52.1.43Suche in Google Scholar

Borsani O., Valpuesta V. & Botella M.A. 2001. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol. 126: 1024–1030.10.1104/pp.126.3.1024Suche in Google Scholar

Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.10.1016/0003-2697(76)90527-3Suche in Google Scholar

Cakmak I. & Marschner H.1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol.98:1222–1227.10.1104/pp.98.4.1222Suche in Google Scholar PubMed PubMed Central

Campos J.M.S. & Vicini L.F. 2003. Cytotoxicity of aluminum on meristematic cells of Zea mays and Allium cepa. Caryologia 56: 65–73.10.1080/00087114.2003.10589309Suche in Google Scholar

Čiamporová M. 2002. Morphological and structural responses of plant roots to aluminum at organ, tissue and cellular levels. Biol. Plant. 45: 161–171.10.1023/A:1015159601881Suche in Google Scholar

Dat J.F., Lopez-Delgado H., Foyer C.H. & Scott I.M. 1998. Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiol. 116: 1351–1357.10.1104/pp.116.4.1351Suche in Google Scholar PubMed PubMed Central

Delhaize E.R.P. 2004. Engineering high-level aluminum tolerance in barley with the ALMT1 gene. PNAS 101:15249–15254.10.1073/pnas.0406258101Suche in Google Scholar PubMed PubMed Central

Drazic G.& Mihailovic N. 2005. Modification of cadmium toxicity in soybean seedlings by salicylic acid. Plant Sci. 168: 511–517.10.1016/j.plantsci.2004.09.019Suche in Google Scholar

Fiskesjö G. 1988. The Allium test as an alternative in environmental studies: The relative toxicity of metal ions. Mutat. Res. 197: 243–260.10.1016/0027-5107(88)90096-6Suche in Google Scholar

Foy C.1992. Soil chemical factors limiting plant root growth. Adv. Soil Sci. 19: 97-149.10.1007/978-1-4612-2894-3_5Suche in Google Scholar

Guo B., Liang Y. & Zhu Y. 2009. Does salicyclic acid regulate antioxidant defense system, cell death, cadmium uptake and partitioning to acquire cadmium tolerance in rice? J. Plant Physiol. 166: 20–31.10.1016/j.jplph.2008.01.002Suche in Google Scholar PubMed

Hameed A., Malik S.A., Iqbal N., Arshad R. & Farooq S. 2004. A rapid (100 min) method for isolating high yield and quality DNA from leaves, roots and coleoptile of wheat (Triticum aestivum L.) suitable for apoptotic and other molecular studies. Int. J. Agric. Biol. 2: 383–387.Suche in Google Scholar

Huang W.J., Oo T.L., He H.Y., Wang A.Q., Zhan J., Li C.Z., Wei S.Q. & He L.F. 2014. Aluminum induced rapidly mitochondria dependent programmed cell death in Al sensitive peanut root tips. Bot. Stud. 55: 67–78.10.1186/s40529-014-0067-1Suche in Google Scholar PubMed PubMed Central

Hue N.V., Craddock G.R. & Adams F.1986. Effect of organic acids on aluminum toxicity in subsoils. Soil Sci. Soc. Amer. J. 50: 28–34.10.2136/sssaj1986.03615995005000010006xSuche in Google Scholar

Janda T., Szalai G., Tari I. & Páldi E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta 208: 175–180.10.1007/s004250050547Suche in Google Scholar

Jones D.L. & Kochian L.V. 1995. Aluminum inhibition of the inositol 1-4-5-trisphosphate signal transduction pathway in wheat roots: A role in aluminum toxicity? Plant Cell 7:1913–1922.10.2307/3870198Suche in Google Scholar

Larkindale J. & Knight M.R. 2002. Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol. 128:682–695.10.1104/pp.010320Suche in Google Scholar PubMed PubMed Central

Li Z. & Xing D. 2010. Mitochondrial pathway leading to programmed cell death induced by aluminum phytotoxicity in Arabidopsis. Plant Signal. Behav. 5: 1660–1662.10.4161/psb.5.12.14014Suche in Google Scholar PubMed PubMed Central

Loake G. & Grant M. 2007. Salicylic acid in plant defence – the players and protagonists. Curr. Opin. Plant Biol.10: 466–472.10.1016/j.pbi.2007.08.008Suche in Google Scholar

Ma J.F., Chen Z.C. & Shen R.F. 2014. Molecular mechanism of Al tolerance in gramineous plants. Plant Soil. 381: 1–12.10.1007/s11104-014-2073-1Suche in Google Scholar

Mahendranath M., Santosh C., Mohan M., Ramesh L. & Radhaiah A. 2012. Protective effect of salicylic acid on aluminium induced stress in Sorghum bicolor varieties. Ind. J. Plant Sci. 2: 99–104.Suche in Google Scholar

Matsumoto H. 2000. Cell biology of aluminum toxicity and tolerance in higher plants. Int. Rev. Cytol. 200: 1–46.10.1016/S0074-7696(00)00001-2Suche in Google Scholar

Metwally A., Finkemeier I., Georgi M. & Dietz K.J. 2003. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol. 132: 272–281.10.1104/pp.102.018457Suche in Google Scholar

Mishra A. & Choudhuri M.A. 1999. Monitoring of phytotoxicity of lead and mercury from germination and early seedling growth indices in two rice cultivars water, air, and soil pollution. 114: 339–346.Suche in Google Scholar

Muñoz-Sánchez A., Altúar-Molina A.R. & Hernández-Sotomayor S.M.T. 2013. Phospholipase signaling is modified differentially by phytoregulators in Capsicum chinense cells. Plant Signal. Behav. 79: 1103–110510.4161/psb.21220Suche in Google Scholar

Ownby J.D. 1993. Mechanism of reaction of hematoxylin with aluminium treated wheat roots. Physiol. Plant 87: 371–381.10.1111/j.1399-3054.1993.tb01744.xSuche in Google Scholar

Pál M., Szalai G., Horvath E., Janda T. & Paldi E. 2002. Effect of salicylic acid during heavy metal stres. Proc. 7th Hungarian Congress on Plant Physiology. Acta Biolog. Szeged. 46:119–120.Suche in Google Scholar

Pandey P., Srivastava R.K. & Dubey R.S. 2013. Salycilic acid alleviates aluminum toxicity in rice seedlings better than magnesium and calcium by reducing aluminum uptake, suppressing oxidative damage and increasing antioxidative defense. Ecotoxicology 22: 656–670.10.1007/s10646-013-1058-9Suche in Google Scholar

Petrov V., Hille J., Mueller-Roeber B. & Gechev T.S. 2015. ROS mediated abiotic stress-induced programmed cell death in plants. Front. Plant Sci. 6: 69.10.3389/fpls.2015.00069Suche in Google Scholar

Roy A.K., Sharma A. & Talukder G. 1989. A time course study on effects of aluminium on mitotic cell division in Allium sativum. Mutat. Res. 227: 221–226.10.1016/0165-7992(89)90100-0Suche in Google Scholar

Schildknecht P.H.P.A. & De CamposVidal B. 2002. A role for the cell wall in Al+3 resistance and toxicity: Crystallinity and availability of negative charges. Int. Arch. Biosci. 2000:1087–1095.Suche in Google Scholar

Senaratna T., Touchell D., Bunn E. & Dixon K. 2000. Acetyl salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul.30:157–161.10.1023/A:1006386800974Suche in Google Scholar

Shakirova F., & Bezrukova M.1997. Induction of wheat resistance against environmental salinization by salicylic acid. Biol. Bull.24:109–112.Suche in Google Scholar

Sharma Y.K., Leon J., Raskin I. & Davis K.R. 1996. Ozone-induced responses in Arabidopsis thaliana: The role of salicylic acid in the accumulation of defense-related transcripts and induced resistance. Proc. Natl. Acad. Sci. USA. 93: 5099–5104.10.1073/pnas.93.10.5099Suche in Google Scholar

Song H., Xu X., Wang H. & Tao Y. 2011. Protein carbonylation in barley seedling roots caused by aluminum and proton toxicity is suppressed. Russ. J. Plant Physiol.58: 653–659.10.1134/S1021443711040169Suche in Google Scholar

Srivastava S. & Dubey R.S. 2011.Manganese-excess induces oxidative stress, lowers the pool of antioxidants and elevates activities of key antioxidative enzymes in rice seedlings. Plant Growth Regul.64:1–16.10.1007/s10725-010-9526-1Suche in Google Scholar

Srivastava M.K. & Dwivedi U.N. 1998. Salicylic acid modulates glutathione metabolism in pea seedlings. J. Plant Physiol. 153: 409–414.10.1016/S0176-1617(98)80168-5Suche in Google Scholar

Strobel N.E. & Kuc J.A. 1995. Chemical and biological inducers of systemic resistance to pathogens protect cucumber and tobacco plants from damage caused by paraquat and cupric chloride. Phytopathology 85: 1306–1310.10.1094/Phyto-85-1306Suche in Google Scholar

Surapu V., Ediga A. & Meriga B. 2014. Salicylic acid alleviates aluminum toxicity in tomato seedlings (Lycopersicum esculentum Mill.) through activation of antioxidant defense system and proline biosynthesis. Adv. Biosci. Biotechnol. 5: 777–789.10.4236/abb.2014.59091Suche in Google Scholar

Uysal D., (Ozdener Y., Aydin B. & Demir E. 2009. Determination of ameliorating effect of salicylic acid on toxicity of aluminum in wheat roots. Fresen. Environ. Bull.18: 32–39.Suche in Google Scholar

Vardar F., Arican E. & Gözükirmizi N. 2006. Effects of aluminum on in vitro root growth and seed germination of tobacco (Nicotiana tabacum L.). Adv. Food Sci. 28: 85–88.Suche in Google Scholar

Vardar F., Akgül N., Aytürk Ö. & Aydin Y. 2015. Assessment of aluminum induced genotoxicity with comet assay in wheat, rye and triticale roots. Fresen. Environ. Bull.37: 3352–3358.Suche in Google Scholar

Vardar F., Çabuk E., Aytürk Ö. & Aydin Y. 2016. Determination of aluminum induced programmed cell death characterized by DNA fragmentation in Gramineae species. Caryologia 69: 111–115.10.1080/00087114.2015.1109954Suche in Google Scholar

Vardar F., İsmailoğlu İ., İnan D. & Ünal M. 2011. Determination of stress responses induced by aluminum in maize (Zea mays). Acta Biol. Hung. 62: 156–170.10.1556/ABiol.62.2011.2.6Suche in Google Scholar PubMed

Vardar F. & Ünal M. 2007. Aluminum toxicity and resistance in higher plants. Adv. Mol. Biol. 1: 1–12.Suche in Google Scholar

Wang Y.S., Wang J., Yang Z.M., Wang Q.Y., Lü B., Li S.Q., Lu Y.P., Wang S.H. & Sun X. 2004. Salicylic acid modulates aluminum-induced oxidative stress in roots of Cassia tora. Acta Bot. Sin. 46: 819–828.Suche in Google Scholar

Wang L.J. & Li S.H. 2006. Salicylic acid-induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant systems in young grape plants. Plant Sci. 170: 685–694.10.1016/j.plantsci.2005.09.005Suche in Google Scholar

War A.R., Paulraj G., War M.Y. & Ignacimuthu S. 2011. Role of salicylic acid in induction of plant defense system in chickpea (Cicer arietinum L.). Plant Signal. Behavior. 6: 1787–1792.10.4161/psb.6.11.17685Suche in Google Scholar PubMed PubMed Central

Yamamato Y., Kobayashi Y. & Matsumoto H. 2001. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea root. Plant Physiol. 125: 199–208.10.1104/pp.125.1.199Suche in Google Scholar PubMed PubMed Central

Yang Z.M., Wang J. & Wang S.H. 2003. Salicylic acid-induced aluminium tolerance by modulation of citrate efflux from roots of Cassia tora L. Planta 217: 74.10.1007/s00425-003-0980-0Suche in Google Scholar PubMed

Zhang L., Xu Q., Xing D., Gao C. & Xiong H. 2009. Real time detection of caspase-3 like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure. Plant Physiol.150: 1773–1783.10.1104/pp.108.125625Suche in Google Scholar PubMed PubMed Central

Zhou Z.S., Guo K., Elbaz A.A. & Yang Z.M. 2009. Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ. Exp. Bot. 65: 27–34.10.1016/j.envexpbot.2008.06.001Suche in Google Scholar

Received: 2016-7-20
Accepted: 2016-9-15
Published Online: 2017-1-12
Published in Print: 2016-12-1

© 2016 Institute of Botany, Slovak Academy of Sciences

Heruntergeladen am 9.12.2025 von https://www.degruyterbrill.com/document/doi/10.1515/biolog-2016-0166/pdf
Button zum nach oben scrollen