Analytical evaluation of the Dade Behring Dimension RxL automated N-Terminal proBNP (NT-proBNP) method and comparison with the Roche Elecsys 2010
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Francesca Di Serio
, Vincenzo Ruggieri , Lucia Varraso , Rosalisa De Sario , Angela Mastrorilli und Nicola Pansini
Abstract
Methods to quantify B-type natriuretic peptide (BNP) and N-terminal-propeptide (NT-proBNP) in plasma or serum samples are well established. We assessed the analytical performance of the Dimension RxL NT-proBNP method (Dade-Behring). Evaluation of different sample types was carried out. Controls and heparin plasma pools were used to determine the detection limit, precision, and linearity. Sample stability and the effect of interfering substances on the NT-proBNP concentrations were evaluated. Agreement between Dimension RxL and Elecsys 2010 (Roche Diagnostics) NT-proBNP methods was assessed. The influence of age and sex on NT-proBNP concentrations was evaluated in healthy subjects. Heparin plasma should be the matrix of choice. The detection limit was 2.0ng/L. The total imprecision was 2.6–3.6% for concentrations from 231 to 9471ng/L; mean NT-proBNP concentrations of 21 and 15ng/L were associated with coefficients of variation of 9.9% and 14.7%, respectively. The method was linear up to 32,650ng/L. There was no effect of temperature, freeze-thaw cycles and interfering substances. A bias was detected when Dimension RxL and Elecsys 2010 NT-proBNP methods were compared. Age and sex were significantly and independently related to NT-proBNP concentrations. The Dimension RxL NT-proBNP method, like the Elecsys 2010, is suitable for routine use in the diagnosis of heart failure.
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Artikel in diesem Heft
- Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region
- Isotretinoin therapy induces DNA oxidative damage
- Structural evaluation of plasma α2-macroglobulin in acute pancreatitis
- Failure of the PAXgene™ Blood RNA System to maintain mRNA stability in whole blood
- Reticulocyte hemoglobin measurement – comparison of two methods in the diagnosis of iron-restricted erythropoiesis
- Physical analysis of ejaculate to evaluate the secretory activity of the seminal vesicles and prostate
- Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma
- Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice
- Plasma levels of 7-hydroxymetabolites of dehydroepiandrosterone in healthy Central European aging men
- Prevalence of abnormal thyroid stimulating hormone and thyroid peroxidase antibody-positive results in a population of pregnant women in the Samara region of the Russian Federation
- Impact of standardized calibration on the inter-assay variation of 14 automated assays for the measurement of creatinine in human serum
- Efficacy of three ELISA measurements of anti-cyclic citrullinated peptide antibodies in the early diagnosis of rheumatoid arthritis
- Mean and variance quality control for multiple correlated levels of replicated control samples
- Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
- Analytical evaluation of the Dade Behring Dimension RxL automated N-Terminal proBNP (NT-proBNP) method and comparison with the Roche Elecsys 2010
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- Reportable interval of patient examination results and ISO 15189