Isotretinoin therapy induces DNA oxidative damage
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Sophia Georgala
Abstract
Background: Isotretinoin (Iso) is currently indicated for the treatment of cystic acne (CA) and is related to marked teratogenicity.
Aim: The aim of the study was to evaluate the relationship between total antioxidant status (TAS) and a serum marker of DNA oxidative damage, 8-hydroxy-2-desoxyguanosine (8-OHdG), in patients on Iso treatment.
Patients and methods: Patients with CA (n=18) were evaluated before and 45days after Iso (0.5mg/kg per day) treatment and non-diseased controls (n=22) were tested only once. Plasma TAS levels and 8-OHdG were measured spectrophotometrically and with an immunoassay, respectively. Liver biochemical parameters and muscle enzymes were measured on a blood chemistry analyzer.
Results: TAS levels were significantly (p<0.0001) lower in patients before treatment (921±124μmol/L) compared with those after treatment (1335±93μmol/L) and in controls (1536±126μmol/L). In contrast, 8-OHdG serum levels were two-fold higher in patients after treatment (0.21±0.03ng/mL) than before treatment (0.11±0.02ng/mL) and three-fold higher than in controls (0.07±0.01ng/mL; p<0.0001). Negative correlations were found between TAS and 8-OHdG (r=−0.754, p<0.0001) in patients before therapy and positive correlations were found between creatine kinase (CK) and 8-OHdG (r=0.488, p<0.001) and liver enzymes after Iso treatment.
Conclusions: High serum levels of 8-OHdG in patients on Iso therapy may be due to a direct effect of Iso on liver, muscle and skin epidermal cells. Regular evaluation of 8-OHdG in sera of patients, especially of women of reproductive age, on Iso treatment could be a sensitive follow-up biomarker of DNA oxidation.
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©2005 by Walter de Gruyter Berlin New York
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Artikel in diesem Heft
- Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region
- Isotretinoin therapy induces DNA oxidative damage
- Structural evaluation of plasma α2-macroglobulin in acute pancreatitis
- Failure of the PAXgene™ Blood RNA System to maintain mRNA stability in whole blood
- Reticulocyte hemoglobin measurement – comparison of two methods in the diagnosis of iron-restricted erythropoiesis
- Physical analysis of ejaculate to evaluate the secretory activity of the seminal vesicles and prostate
- Specificity of the 3H-triolein assay for pancreatic lipase in blood plasma
- Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice
- Plasma levels of 7-hydroxymetabolites of dehydroepiandrosterone in healthy Central European aging men
- Prevalence of abnormal thyroid stimulating hormone and thyroid peroxidase antibody-positive results in a population of pregnant women in the Samara region of the Russian Federation
- Impact of standardized calibration on the inter-assay variation of 14 automated assays for the measurement of creatinine in human serum
- Efficacy of three ELISA measurements of anti-cyclic citrullinated peptide antibodies in the early diagnosis of rheumatoid arthritis
- Mean and variance quality control for multiple correlated levels of replicated control samples
- Clinical evaluation of serodiagnosis of active tuberculosis by multiple-antigen ELISA using lipids from Mycobacterium bovis BCG Tokyo 172
- Analytical evaluation of the Dade Behring Dimension RxL automated N-Terminal proBNP (NT-proBNP) method and comparison with the Roche Elecsys 2010
- Unusually high alkaline phosphatase due to intestinal isoenzyme in a healthy adult
- Deficient α-galactosidase A activity in plasma but no Fabry disease – a pitfall in diagnosis
- Reportable interval of patient examination results and ISO 15189
